Expression of nuclear factor erythroid 2-related factor 2 in term prelabor rupture of membranes and its clinical significance
LIU Jia-jia1, YU Jun1, XIE Bing2, FANG Jie1, LUAN Xiao-jin1, YAN Yi-dan1
(1. Department of Obstetrics and Gynecology, Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001; 2. Department of Obstetrics and Gynecology, the Fourth People′s Hospital Affiliated to Jiangsu University, Zhenjiang Jiangsu 212001, China)
Abstract：Objective: To investigate the relationship between the expression of nuclear erythroid 2related factor 2 (Nrf2) in fetal membranes and the occurrence and development of term prelabor rupture of membrane. Methods: A total of 30 healthy pregnant women as the control group and 30 patients with prelabor rupture of membranes were selected. The expression of Nrf2 mRNA and protein in fetal membranes were detected by qRTPCR and Western blotting. The location and expression of Nrf2 in fetal membranes were detected by immunohistochemistry. Results: Compared with the control group, the expression of Nrf2 mRNA and protein were significantly down-regulated in the prelabor rupture of membranes group(P<0.01 and P<0.05). Immunohistochemistry illustrated that Nrf2 was expressed in the nucleus and cytoplasm of all membrane structures. Compared with the control group, the expression of Nrf2 protein was markedly decreased in the amnion epithelial layer, chorionic trophoblast layer and decidua layer of the prelabor rupture of membranes group(P<0.05 or P<0.01). The expression of Nrf2 was highest in the amnion epithelial layer. Conclusion: The lower expression of Nrf2 in fetal membranes may promote the occurrence and development of prelabor rupture of membranes.
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2019, Vol. 29 
