目的: 探讨胎膜组织中核因子E2相关因子2(nuclear factor erythroid 2-related factor 2, Nrf2)的表达与足月胎膜早破发生发展的关系。方法: 选取30例健康孕妇(对照组)和30例胎膜早破孕妇(胎膜早破组)，采用荧光定量PCR和蛋白质印迹分别检测两组胎膜组织中Nrf2 mRNA及其蛋白的表达；免疫组织化学检测胎膜组织中Nrf2定位及表达。结果： 与对照组比较，胎膜早破组Nrf2 mRNA及蛋白表达明显降低(P<0.01和P<0.05)；Nrf2在胎膜组织各层结构的细胞核和细胞质中均可见，Nrf2在羊膜上皮层表达最高；与对照组比较，胎膜早破组Nrf2在羊膜上皮层、绒毛滋养细胞层和蜕膜层中表达明显下降(P<0.05或P<0.01)。结论： 胎膜组织中Nrf2低表达可能与胎膜早破的发生发展有关。
Abstract
Objective: To investigate the relationship between the expression of nuclear erythroid 2related factor 2 (Nrf2) in fetal membranes and the occurrence and development of term prelabor rupture of membrane. Methods: A total of 30 healthy pregnant women as the control group and 30 patients with prelabor rupture of membranes were selected. The expression of Nrf2 mRNA and protein in fetal membranes were detected by qRTPCR and Western blotting. The location and expression of Nrf2 in fetal membranes were detected by immunohistochemistry. Results: Compared with the control group, the expression of Nrf2 mRNA and protein were significantly down-regulated in the prelabor rupture of membranes group(P<0.01 and P<0.05). Immunohistochemistry illustrated that Nrf2 was expressed in the nucleus and cytoplasm of all membrane structures. Compared with the control group, the expression of Nrf2 protein was markedly decreased in the amnion epithelial layer, chorionic trophoblast layer and decidua layer of the prelabor rupture of membranes group(P<0.05 or P<0.01). The expression of Nrf2 was highest in the amnion epithelial layer. Conclusion: The lower expression of Nrf2 in fetal membranes may promote the occurrence and development of prelabor rupture of membranes.
关键词
胎膜早破 /
核因子E2相关因子2 /
抗炎 /
诊断 /
预测价值
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参考文献
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脚注
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基金
镇江市社会发展基金资助项目(SH2017013，SH2017020，SH2016028)；镇江市科技卫生重点研发项目(SHW2016001)；2017年镇江市社会发展指导性项目(FZ2017037)；江苏大学附属医院博士科研启动基金资助项目(jdfyRC2016005)
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