Abstract：Objective: To explore the inhibition effect of human umbilical cordderived mesenchymal stem cell culture supernatant on aging of human skin fibroblasts. Methods: Primary human dermal fibroblasts were divided into four groups:normal control group,H2O2treated group, culture supernatanttreated group and H2O2+culture supernatanttreated group.To induce cell senescence，the cells in H2O2treated group and H2O2 + culture supernatanttreated group were treated with 200 μmol/L H2O2 for 4 h.The βgalactosidase activity and the nuclear volume were detected by βgalactosidase staining kit and DAPI staining respectively. The mRNA transcription levels of P53 and P21 in cells were detected with realtime fluorescent quantitative PCR(RTqPCR). The protein expression level of P53 was detected with Western blotting. Results: Compared with the normal control group, the βgalactosidase activity associated with cell senescence was significantly enhanced after being treated with H2O2, and the percentage of βgalactosidase positive cells was significantly increased(P<0.05);the nuclear volume was significantly bigger(P<0.05); the transcription levels of P53 and P21 mRNA were significantly increased (P<0.05), and the expression of P53 protein was enhanced. After being treated with mesenchymal stem cell culture supernatant, the abovementioned agingrelated indicators were significantly ameliorated (P<0.05). Conclusion: Human umbilical cordderived mesenchymal stem cell culture supernatant inhibits H2O2induced fibroblast senescence significantly.
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2019, Vol. 29 
