Abstract：Objective: To investigate the effect of hepatocyte growth factor(HGF) on doxorubicin(DOX)induced H9C2 cells apoptosis. Methods: The third generation of mesenchymal stem cells(MSCs) were divided into three groups:cultured alone,HGF-siRNA-MSCs and NC-siRNA-MSCs.The concentration of HGF and transforming growth factor β1(TGF-β1) were measured with both ELISA and Western-blot kit.H9C2 cells were exposed to 1.0 μmol/L doxorubicin for 4 hours.Then they were divided into four groups: cultured alone,co-cultured with MSCs,co-cultured with HGF-siRNA-MSCs and co-cultured with NC-siRNA-MSCs.After 24 hours，the apoptosis rate was determined by flow cytometry (FCM). Results: Compared with other groups，the concentration of TGF-β1 in MSCs were significantly increased in the HGF-siRNA-MSCs[(519.23±24.34）pg/mL］ than cultured alone\[（459.65±11.78）pg/mL] and NC-siRNA-MSCs [（459.33±11.78）pg/mL,P<0.05］.The apoptosis rate of H9C2 cells was increased significantly in co-cultured with HGF-siRNA-MSCs（18.54±0.64)% than co-cultured with MSCs(6.65±0.49)% and NC-siRNA-MSCs ［(9.70 ± 1.62)%,P<0.05］. Conclusion: HGF prevented DOX-induced H9C2 cellsapoptosis by inhibition of TGF-β1.
