Abstract：［Abstract］Objective： To investigate the influence of secreted protein acidic and rich in cysteineslike 1（SPARCL1） gene on the proliferation and apoptosis of human hepatocellular carcinoma SMMC7721 cell. Methods： SMMC7721 cells were divided into five groups, including blank control group, pIRES2ZsGreen vector group, pIRES2ZsGreenSPARCL1 group, negative siRNA group and SPARCL1 siRNA group. Western blotting was used to investigate SPARCL1 expression, while fluorescence microscope were used to verify the transfection efficiency of SPARCL1. MTT assay was used to detect cell proliferation ability; and flow cytometry was used to measure apoptosis rate. Results： The transfection efficiency of SPARCL1 was high through the fluorescence microscope observation and western blotting. SPARCL1 expression, the cell inhibition ratio, and the apoptosis ratio in pIRES2 ZsGreenSPARCL1 group was significantly higher than other four groups(P<0.05), while SPARCL1 siRNA group was lower than other four groups(P<0.05). Conclusion： SPARCL1 overexpression elevated the proliferation inhibition ratio and apoptosis ratio of human hepatocellular carcinoma SMMC7721 cell； on the contrary， SPARCL1 silencing lowered.
韩玮1， 徐旋旋2， 何大伟2， 曹方1， 丁厚中1. SPARCL1基因对肝癌SMMC-7721细胞增殖与凋亡的影响[J]. 江苏大学学报:医学版, 2016, 26(04): 292-295,301.
HAN Wei1, XU Xuan-xuan2, HE Da-wei2, CAO Fang1, DING Hou-zhong1. Influence of SPARCL1 gene on the proliferation and apoptosis of human hepatocellular carcinoma SMMC-7721 cell. Journal of Jiangsu University(Medicine Edition), 2016, 26(04): 292-295,301.
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2016, Vol. 26 
