Abstract：Objective: To observe the effect of podocytes Tbxa2r gene knockdown on podocytes apoptosis induced by puromycin aminonucleoside (PAN). Methods: The interference effect was detected by RTPCR after designing the target sequence and evaluating the cell transfection efficiency. The expression of Tbxa2r protein was measured by western blotting. The apoptosis of MPC5 cells induced by PAN was tested by flow cytometry. Results: Target sequence after 24 h transfection almost had no interference effects, and after 48 h, the third siRNA (Tbxa2rmus1677) had the best interference effect; after 72 h transfection, Tbxa2r protein expression was significantly reduced. PAN stimulated for 48 h, no significant MPC5 podocyte apoptosis were observed in the control group and negative control group, while there were significant apoptosis and necrosis in the positive control group and Tbxa2r gene knockdown PAN group. Conclusion: The PAN might facilitate the apoptosis and necrosis in Tbxa2r gene knockdown podocytes.
2014, Vol. 24 
