Role of endoplasmic reticulum stress in activation-induced cell death of T cells
YAN Cheng 1,2, JIN Hui-min 1 ,2, YAN Nan-nan 1,2, XU Jie 1,2, XIAO Teng-fei 1,2, ZHOU Li-ping 3, SHAO Qi-xiang 1,2, XIA Sheng 1,2
（1. Department of Immunology, 2. Institute of Clinic Laboratory Diagnosis, 3. Department of Molecular Biology, School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013, China)
Abstract：Objective: To explore the role of endoplasmic reticulum（ER） stress in activation-induced cell death(AICD) of T cells. Methods: The model of AICD was set up by using EL-4 cells(mice T lymphoma cell) and the T cell of adult C57BL/6 mice， the cell apoptosis of AICD was measured by flow cytometry. The effect of CD3 antibody concentrations on EL-4 cell AICD was assessed by flow cytometry. Flow cytometry was used to observe and analyze the apoptotic cells of agonist group which added with ER stress inducer dithiothreitol and inhibition group which added with ER stress inhibitor 4-phenylbutyric acid in T cell AICD of adult C57BL/6 mice. Results: The cell apoptosis was increased when AICD formed in EL-4 cell and T cell in C57BL/6 mice.With the increase of CD3 antibody concentration, the occurrence rate of EL-4 cell AICD increased. Dithiothreitol induced the occurrence of T cell AICD in mice, while 4-phenylbutyric acid reduced the occurrence of T cell AICD in mice. Conclusion: The induction of ER stress could promote T cell AICD, while inhibition of ER stress decrease T cell AICD. ［Key words］endoplasmic reticulum stress; T cell; activationinduced cell death
2017, Vol. 27 
