Abstract：Objective: To investigate the influence of Fumonisin B1(FB1) on the proliferation of HepG2 cells and its possible mechanism.Methods: HepG2 cells were cultured 48 h in prepared DMEM with different concentrations of 0.5, 1.0,10, 50 μmol/L FB1, the morphology of each group were observed under inverted microscope. Cell proliferation was evaluated by Cell Counting Kit 8(CCK-8)assay. The mRNA expression levels of transforming growth factor-β1（TGF-β1), Drosophila mothers against decapentaplegic 4(Smad4) and plasminogen activator inhibitor-1(PAI-1) were detected by quantitative real-timePCR. Results: Microscope showed that cell division was increased in parallel with the increase of FB1 concentration. CCK-8 assay revealed that cell proliferation was remarkably increased after treatment of FB1. In the 0.5 and 1.0 μmol/L groups, mRNA expression of Smad4 and PAI-1 in HepG2 cells showed no significant difference compared with control group, while in the 10 and 50 μmol/L groups were increased dramatically. Conclusion: FB1 could promote cell proliferation effectively, and the elevated level of TGF-β1, Smad4 and PAI-1 in TGF-β pathway may be one of mechanisms for promoting cell proliferation in HepG2 cells.
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2014, Vol. 24 
