Abstract：Objective: To explore the influence of flagella rotor protein gene fliG on gene expression regulation of Salmonella enterica serovar Typhi under antiz66 antiserum stress. Methods: The fliG deleted mutant of S. enterica serovar Typhi was prepared by homologous recombination mediated by suicide plasmid; S. enterica serovar Typhi genomic microarray was used to investigate the difference of gene expression between the wild type and fliG mutant; qRTPCR was done to prove the results of microarray. Results: The fliG deleted mutant of S. enterica serovar Typhi was constructed successfully. Gene expression profiles analysis revealed that expression of 21 genes were increased and expression of 7 genes were decreased in the fliG mutant under anti-z66 antiserum stress. Conclusion: The flagella rotor protein gene fliG probably played a role to regulate some gene expression in response to anti-z66 antiserum stress.
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2012, Vol. 22 
