Abstract:Objective: To investigate the effect of small RNA SbfR on the motility and invasion of Salmonella enterica serovar Typhi and its potential mechanism. Methods: The effect of SbfR on the bacterial phenotype was observed using motility assay on swimming agar plates and the invasion assay of HeLa cells. The muted strains were as follows the empty vector strain (WT+pBAD/MycHisA), the SbfR deletion mutant (△SbfR+pBAD/MycHisA) and the SbfR complementary strain (△SbfR+ pBAD/MycHisASbfR). In addition, gene microarray and qRTPCR were used to analyze the regulation of SbfR on the expression of flagella and invasion related genes. Results: Compared to the control strain, the SbfR deletion mutant showed significantly weaker motility and invasion(P<0.01), while the SbfR complementary strain did not cause any change. Furthermore, expressional levels of flagella and invasion related genes in the SbfR deletion mutant were lower than that in the complementary SbfR strain(P<0.05 or P<0.01). Conclusion: SbfR upregulates the transcriptional levels of flagella and invasion related genes, and promotes the motility and invasion of S. Typhi.
收稿日期: 2017-11-16
通讯作者:
黄新祥(通讯作者),教授,博士生导师,Email:huxinx@ujs.edu.cn
作者简介: 孙嘉遥(1991—),女,江苏连云港人,初级检验技师,硕士研究生
引用本文:
孙嘉遥, 陆仁飞, 赵昕, 黄新祥. 小RNA SbfR增强伤寒沙门菌动力和侵袭力[J]. 江苏大学学报:医学版, 2018, 28(01): 47-50.
SUN Jia-yao, LU Ren-fei, ZHAO Xin, HUANG Xin-xiang. Small RNA SbfR promotes motility and invasion of Salmonella enterica serovar Typhi. Journal of Jiangsu University(Medicine Edition), 2018, 28(01): 47-50.
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