基于Fe-CDs的肿瘤细胞荧光成像和
过氧化氢的比色测定

doi:10.3969/j.issn.1671-7775.2024.06.015

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摘要以柠檬酸铁和聚乙烯亚胺（polyethyleneimine，PEI）为初始原料，采用微波水热法，快速合成了具有良好光致发光性能的铁掺杂碳点（Fe doped carbon dots，FeCDs）.研究表明：在较宽的pH范围（pH=3~9）、较高的离子强度和较长的储存时间下，FeCDs表现出优异的稳定性，易穿透细胞膜，并在细胞内聚集，可作为肺癌A549细胞生物成像的有效探针；FeCDs具有优异的类过氧化物酶（peroxidaselike，POD）活性，能够高效催化过氧化氢（H2O2）对3,3′,5,5′-四甲基联苯胺（TMB）的氧化，并生成蓝色氧化产物，基于此建立了H2O2比色测定方法；在最佳反应条件下，H2O2浓度为0~100.00 μmol/L时，H2O2与反应体系的吸光度呈现良好的线性关系，H2O2检出限低至0.13 μmol/L，说明该比色测定方法对H2O2的检测表现出较高的灵敏度和选择性；将该比色测定方法用于A549细胞内H2O2的测定，加样回收率高达98.4%~102.8%，验证了该方法的可靠性.

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	李潇然
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	李峰
	庞中好
	徐秀泉

关键词 ：铁掺杂碳点, 荧光探针, 过氧化氢, 过氧化物纳米酶, 比色测定

Abstract：The novel Fe doped carbon dots (FeCDs) with good photoluminescent properties were rapidly synthesized by microwaveassisted method with ferric citrate and polyethyleneimine as raw materials. The results show that the FeCDs have good stability under the conditions of broad pH range from 3 to 9, high ion strengths and long storage time. The prepared FeCDs can easily penetrate and accumulate into the cell, which can be used as effective probe for biological imaging of A549 cells. The FeCDs display distinct peroxidaselike (POD) activities, which is evidenced by the catalyzed oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxidized products in the presence of H2O2. A colorimetric method for the determination of H2O2 is established based on the results. Under optimal reaction conditions, the colorimetric method for H2O2 detection is constructed with good linear relationship from 0 to100.00 μmol/L and detection limit down to 0.13 μmol/L. The highly selective and ultrasensitive method is successfully applied to detect H2O2 inside A549 tumor cells with good recoveries of 98.4%-102.8%, which can verify the proposed method.

Key words： Fe doped carbon dot fluorescent probe hydrogen peroxide peroxidase mimetic nanozyme colorimetric detection

作者简介: 李潇然（1997—），男，江苏淮安人，硕士，住院医师（J18852866858@163.com）,主要从事心胸外科肿瘤的诊断与治疗研究. 徐秀泉（1978—），男，山东滨州人，博士，副教授（通信作者，xxq781026@ujs.edu.cn），主要从事纳米材料合成与传感研究.

引用本文:

李潇然，吴春燕，李峰，庞中好，徐秀泉. 基于Fe-CDs的肿瘤细胞荧光成像和过氧化氢的比色测定[J]. 江苏大学学报（自然科学版）, 2024, 45(6): 732-738. LI Xiaoran, WU Chunyan, LI Feng, PANG Zhonghao, XU Xiuquan. Fluorescence bioimaging and colorimetric detection of H2O2 in tumor cells based on Fe-CDs nanozymes [J]. Journal of Jiangsu University(Natural Science Eidtion) , 2024, 45(6): 732-738.

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http://zzs.ujs.edu.cn/xbzkb/CN/10.3969/j.issn.1671-7775.2024.06.015 或 http://zzs.ujs.edu.cn/xbzkb/CN/Y2024/V45/I6/732

［1］	JIANG X Y, WANG H J, YUAN R, et al. A novel functional 3D porous conductive polymer hydrogels for sensitive electrochemiluminescence in situ detection of H2O2 released from live cells[J]. Analytical Chemistry, DOI: 10.1021/acs.analchem.8b0116.
［2］	范阿强,王晓谦,杨万里,等. 铜依赖性细胞死亡在消化系统肿瘤中的研究进展[J]. 江苏大学学报（医学版）, 2023,33(4):352-358.
	FANG A Q, WANG X Q, YANG W L, et al. Research progress of cuproptosis in digestive system tumors[J]. Journal of Jiangsu University (Medicine Edition), 2023,33(4):352-358.(in Chinese)
［3］	GAO Y, KONG D R, ZHANG Z Y, et al. Spinel CoMn2O4 hollow nanospheres for very wide linear and sensitive detection of hydrogen peroxide[J]. Journal of Alloys and Compounds, DOI:10.1016/j.jallcom.2021.163158.
［4］	张欣,高艳芳. 基于二硫化钼纳米酶的比色法检测过氧化氢[J]. 分析化学, 2022,50(10):1491-1501.
	ZHANG X, GAO Y F. Molybdenum disulfide nanozymes for colorimetric determination of hydrogen peroxide[J]. Chinese Journal of Analytical Chemistry, 2022,50(10):1491-1501.(in Chinese)
［5］	LYU Z Y, ZHOU J C, DING S C, et al. Recent advances in singleatom nanozymes for colorimetric biosensing[J]. Trends in Analytical Chemistry, DOI:10.1016/j.trac.2023.117280.
［6］	HE D M, YAN M M, SUN P J, et al. Recent progress in carbondotsbased nanozymes for chemosensing and biomedical applications[J]. Chinese Chemical Letters, 2021,32:2994-3006.
［7］	WARBURG O. On the origin of cancer cells [J]. Science, 1956,123:309-314.
［8］	GUI U, KANWAL S, TABASSUM S, et al. Microwaveassisted synthesis of carbon dots as reductant and stabilizer for silver nanoparticles with enhancedperoxidase like activity for colorimetric determination of hydrogen peroxide and glucose[J]. Microchimica Acta, DOI:10.1007/s006040194098x.
［9］	LIU H H, LI Y J, YUAN M W, et al. Solid and macroporous Fe3C/NC nanofibers with enhanced electromagnetic wave absorbability[J]. Scientific Reports, DOI:10.1038/s4159801835078z.
［10］	WANG X L, LU Y G, HUA K W, et al. Iodinedoped carbon dots with inherent peroxidase catalytic activity for photocatalytic antibacterial and wound disinfection[J]. Analytical and Bioanalytical Chemistry, 2021,413(5):1373-1382.
［11］	XU H, YE Q, ZHANG J, et al. Oxygen functionalized g-C3N4 strengthen Fe (III)/H2O2 system by accelerating Fe (III)/Fe (II) cycles under natural solar light: a mutualpromoting configuration[J]. Science of the Total Environment, DOI:10.1016/j.scitotenv.2021.146280.
［12］	CUI F C, LI L L, WANG D F, et al. Fe/Ndoped carbon dotsbased nanozyme with super peroxidase activity, high biocompatibility and antibiofilm ability for food preservation[J]. Chemical Engineering Journal, DOI:10.1016/j.cej.2023.145291.
［13］	ZHANG J Y, LIN Y, WU S H, et al. Selfphotooxidation for extending visible light absorption of carbon dots and oxidaselike activity[J]. Carbon, 2021,182:537-544.
［14］	LIU W D, CHU L, ZHANG C H, et al. Heminassisted synthesis of peroxidaselike FeNC nanozymes for detection of ascorbic acidgenerating bioenzymes[J]. Chemical Engineering Journal, DOI:10.1016/j.cej.2021.128876.
［15］	TAN H L, MA C J, GAO L, et al. Metalorganic frameworkderived copper nanoparticle@carbon nanocomposites as peroxidase mimics for colorimetric sensing of ascorbic acid[J]. Chemistry: a European Journal, DOI:10.1002/chem.201404960.
［16］	JIN H, YE D X, SHEN L H, et al. Perspective for single atom nanozymes based sensors: advanced materials, sensing mechanism, selectivity regulation, and applications[J]. Analytical Chemistry, 2022,94:1499-1509.