Abstract： A near-infrared region (NIR) ratiometric fluorescent probe Cy-P based on hemicyanine was rationally designed, prepared and utilized for the detection of exogenous and endogenous peroxynitrite（ONOO-） in living cells. The research results show that Cy-P itself displays NIR emission owing to the large π-conjugated system. With the addition of the strong oxidizer ONOO-, the π-conjugated system of Cy-P is destroyed, and a new substance with blue fluorescence is formed, leading to 268 nm blueshift of fluorescent emission. The linear detection range of the probe Cy-P to ONOO- is 0-15 μmol/L, and the detection limit is 13 nmol/L. The probe exhibits superior selectivity for ONOO- over various potentially interfering biological analytes. Due to the low cytotoxicity, the prepared probe is suitable for imaging detection of exogenous and endogenous ONOO- in living cells.
[J]. 江苏大学学报（自然科学版）, 2024, 45(1): 112-118.
HAN Zhixiang, DAI Xiaoting, XIONG Jie. A near-infrared region ratiometric fluorescent probe for peroxynitrite detection[J]. Journal of Jiangsu University(Natural Science Eidtion)
, 2024, 45(1): 112-118.
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