Abstract：The 7-(diethylamino)-N′-(2,4-dinitrophenyl)-2-oxo-2H-chromene-3-carbohydrazide-based fluorescent probe 1 was reasonably designed, synthesized and employed for the detection of hypochlorous acid (HOCl) in aqueous solution and living cells. The results show that the probe 1 exhibits almost none fluorescence due to the strong quenching effect of dinitrophenyl group and the unique rotation of N—N single bond in acetohydrazide group. With the addition of HOCl, the significant fluorescence enhancement is obtained with notable fluorescence color changing from dark to bright blue. In the presence of 100 μmol?L-1 HOCl, the fluorescence intensity of probe 1 is enhanced 134 times. The relationship between fluorescence intensity of probe 1 at 463 nm and HOCl concentration shows linear enhancement from 0.1 μmol?L-1 to 40.0 μmol?L-1, and the detection limit can reach 0.052 μmol?L-1. Probe 1 shows specific recognition to HOCl with fast response. The probe 1 can be successfully applied to visualize HOCl in living cells with the aid of fluorescence imaging.
韩志湘, 蔡春祥, 张功博, 姜梦云, 李辉. 一种新型高选择性次氯酸荧光探针的研制及其应用[J]. 江苏大学学报（自然科学版）, 2018, 39(5): 586-590.
HAN Zhi-Xiang, CAI Chun-Xiang, ZHANG Gong-Bo, JIANG Meng-Yun, LI Hui. Development and application of a novel fluorescent probe for hypochlorous acid with high selectivity[J]. Journal of Jiangsu University(Natural Science Eidtion)
, 2018, 39(5): 586-590.
2018, Vol. 39 
