Journal of Jiangsu University(Medicine Edition)

[an error occurred while processing this directive]

Journal of Jiangsu University(Medicine Edition)

Home | Instruction for Authors | About Journal | Subscriptions | Advertisement | Contacts Us | 中文

　

Office Online

　

　

　

　

　

　

Editor-in-chief

　

Journal Online

　

　

Forthcoming Articles

　

　

　

　

　

Download Articles

　

　

Quick Search

Advanced Search

Journal of Jiangsu University(Medicine Edition)

	2021 Vol.31 Issue.05 Published 2021-09-30

	0


		2021 Vol. 31 (05): 0- [Abstract] ( 36 ) [HTML 1KB] [ PDF 319KB] ( 431 )

	369	Near-infrared light-responsive composite nanoparticles mediated photothermal ablation and free radical generation for combined inhibition of breast cancer cells
	Near-infrared light-responsive composite nanoparticles mediated photothermal ablation and free radical generation for combined inhibition of breast cancer cells[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(05): 369-373>')" href="#">	ZHANG Jingjing, XU Lixia, LIU Suwan, LIU Jinjing, DU Fengyi, ZHANG Lirong
		Objective: To develop novel polydopamine composite nanoparticles and explore its combined inhibition of breast cancer cells. Methods: Novel polydopamine nanoparticles -AIPH@PB NPs） were prepared by one-step oxidation polymerization using serum albumin（BSA）, 2,2-azobis［2（2imidazoline2yl）-propane- dihydrochloride （AIPH） and dopamine （DA） as precursors. The physical and chemical properties were characterized by Malvern particle size analyzer, Xray photoelectron spectroscopy（XPS） and Fourier infrared spectroscopy. Ultravioletvisible spectroscopy was used to characterize its ability to generate alkyl radicals. The photothermal conversion efficiency was characterized by near infrared thermal imager. The 4T1 breast cancer cells were used to verify the effect of tumor suppression in vitro. Results: AIPH@PB NPs with good biocompatibility and a particle size of 165 nm were successfully prepared. Under the irradiation of 808 nm nearinfrared laser, AIPH@PB NPs could convert light energy into heat energy （photothermal conversion efficiency could be as high as 31%）. Meanwhile, local high temperature further induced the decomposition of AIPH to produce a large number of alkyl radicals. The cell experiment results showed that photothermal ablation and the generation of alkyl radicals mediated by AIPH@PB NPs significantly reduced the activity of 4T1 cells. Conclusion: AIPH@PB NPs was successfully prepared and may， with strong photothermal conversion efficiency and highefficiency alkyl radical generation capacity, be used for combined inhibition of breast cancer cells.
		2021 Vol. 31 (05): 369-373 [Abstract] ( 34 ) [HTML 1KB] [ PDF 2486KB] ( 540 )

	374	#br# Effect of acetyl-CoA synthase 2 interference on the proliferation and apoptosis of human triple-negative breast cancer MDA-MB-468 cell lines
	#br# Effect of acetyl-CoA synthase 2 interference on the proliferation and apoptosis of human triple-negative breast cancer MDA-MB-468 cell lines [J]. Journal of Jiangsu University(Medicine Edition), 2021,31(05): 374-379>')" href="#">	FU Cong1， ZHOU Yuepeng2， YIN Chaoyun3， LING Rui1， PU Xi1， TANG Xiang1， CHEN Deyu1
		Objective: To investigate the effect of acetyl-CoA synthase 2-（ACSS2） on proliferation and apoptosis in triple-negative breast cancer MDAMB468 cell and its potential mechanism. Methods:The mRNA and protein expression of ACSS2 in normal breast epithelial cells MCF-10A and human triplenegative breast cancer MDA-MB-468 cell was detected by quantitative real-time PCR（qRT-PCR） and Western blotting， respectively. SiRNAs specific for ACSS2（siRNA-ACSS2） or a scrambled sequence（siRNA-NC） were designed and synthesized as positive and negative controls for transfection, respectively. SiRNAACSS2 or siRNA-NC was transiently transfected into triplenegative breast cancer MDA-MB-468 cells. The interference efficiency of ACSS2 were assessed by qRT-PCR and Western blotting. The proliferation capacity of MDA-MB-468 before and after conducting the targeted interference with ACSS2 was examined by CCK-8 assay. The apoptosis rate of MDA-MB-468 cells before and after interference was determined, using flow cytometry. Additionally, PI3K/AKT signaling pathway and the apoptosis-related proteins such as cleaved-caspase-3, Bcl-2, Bax and proliferation marker ki-67 were detected by Western blotting. Results: Compared with normal breast epithelial cells MCF-10A, MDA-MB-468 cells were showed with higher levels of the mRNA and protein expression of ACSS2（P<0.01）. Compared with MDA-MB-468 cells without the interference, targeted interference of ACSS2 strongly inhibited its proliferation and remarkably induced the apoptosis in MDA-MB-468 cells（all P<0.01）. The protein expression levels of p-PI3K, p-Akt, Ki-67 and Bcl2 were significantly decreased （all P<0.01），meanwhile，the protein expression levels of cleaved-caspase-3 and Bax were marked increased （all P<0.01）.Conclusion: Interference with acetyl-CoA synthase 2 dramatically inhibit the proliferation of triple-negative breast cancer MDAMB468 cells and significantly promote the apoptosis of breast cancer cells, which may be through PI3K/AKT signaling pathway.
		Guide:
		2021 Vol. 31 (05): 374-379 [Abstract] ( 62 ) [HTML 1KB] [ PDF 1123KB] ( 524 )

	380	miR-376c-3p regulates the proliferation and migration of breast cancer cells via targeting glutamate receptor interacting protein 1
	miR-376c-3p regulates the proliferation and migration of breast cancer cells via targeting glutamate receptor interacting protein 1[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(05): 380-385>')" href="#">	WANG Chao, GAO Yuting, LIU Ziyao, LI Tao, WANG Jingzhe, LIU Lu, XIE Yan, SUN Xiaochun
		Objective: To investigate the expression level, biological function and molecular regulation mechanism of miR-376c3p in breast cancer cells.Methods: The expression level of miR-376c-3p in breast epithelial cell MCF-10A and breast cancer cells was detected by qRT-PCR, and then breast cancer cells MCF7 and MD-MB-231 were transfected with miR-376c-3p mimic.The proliferation was detected by colony formation assay.The migration and invasion were detected by Transwell assay and wound healing assay.Starbase database was used to predict the downstream target gene of miR-376c-3p, and the targeting relationship was verified by Western blotting.Results: Compared with human breast epithelial cell MCF-10A, the expression of miR-376C-3p was significantly down-regulated in breast cancer cells MCF-7 and MDA-MB-231（P<0.01）.Colony formation assay showed that overexpression of miR-376c-3p could inhibit the proliferation of breast cancer cells（all P<0.05）.Transwell assay and wound healing assay showed that the upregulation of miR-376c-3p inhibited the migration of breast cancer cells. Bioinformatics results showed that miR-376c-3p could bind to glutamate receptor interacting protein 1（GRIP1）, and Western blotting assay showed that miR-376c-3p could inhibit the expression of GRIP1 and vimentin, and increase E-cadherin expression in breast cancer cells.Conclusion: The expression of miR-376c-3p was down-regulated in breast cancer cells；overexpression of miR-376c-3p significantly inhibited the proliferation and migration ability of breast cancer cells in vitro, and miR376c3p may regulate the epithelialmesenchymal transition process of breast cancer by targeting GRIP1.
		2021 Vol. 31 (05): 380-385 [Abstract] ( 40 ) [HTML 1KB] [ PDF 2868KB] ( 573 )

	386


		2021 Vol. 31 (05): 386-390 [Abstract] ( 30 ) [HTML 1KB] [ PDF 761KB] ( 708 )

	391	The differential expression and bioinformatics analysis of lncRNA44372 in ovarian cancer
	The differential expression and bioinformatics analysis of lncRNA44372 in ovarian cancer[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(05): 391-395>')" href="#">	DING Jing1, YANG Xinxin1, ZOU Xueqin1, GAO Shujun1, LIU Xue1, PAN Lingli1, ZHANG Qiudan1,ZHAO Yangjing1, LIANG Xiuting2, WANG Hui1, ZHU Yanling2, SHAO Qixiang1
		Objective: To explore the expression of lncRNA44372 and its potential mechanism in the development of ovarian cancer. Methods: The gene chip data GSE119054 of ovarian cancer samples and their control samples was extracted and downloaded from the NCBI GEO database, and the differential expressions of lncRNA in ovarian cancer were analyzed by using the CRN online tool. Annolnc was used to pick out the coexpression genes related to lncRNA44372. Gene Ontology and signal pathway enrichment analysis were performed to draw the outline of the possible biological function of lncRNA44372 in ovarian cancer by the online software David. The divinable ceRNA network of lncRNA44372 was constructed by the starbase, an online analysis tool. The expression of lncRNA44372 was detected by qRT-PCR in ovarian cancer cell lines. Results: In the GSE119054 data set, there were 6 upregulated lncRNA and 4 downregulated lncRNA in ovarian cancer. The related coexpressed genes of lncRNA44372 were enriched in some biological processes, such as cell apoptosis, cell cycle regulation, protein threonine/tyrosine phosphatase activity. The related co-expressed genes of lncRNA44372 were mostly involved in signaling pathways about cell apoptosis and tumor pathogenesis and progression. LncRNA44372 may act as a microRNA sponge to bind with miR190 and miR193 and regulate the expression of ULK2 and LONP2 genes in ovarian cancer. qRT-PCR showed that the expression of lncRNA44372 in ovarian cancer cells was lower than that in normal ovarian cells（P<0.05）. Conclusion: LncRNA44372 was lowly expressed in ovarian cancer and it may play a role in the development and progression of ovarian cancer by influencing cell apoptosis, cell cycle and so on.
		2021 Vol. 31 (05): 391-395 [Abstract] ( 40 ) [HTML 1KB] [ PDF 1176KB] ( 533 )

	396	Effect of self-made Tiaoqi Decoction on cisplatin induced renal injury in rats and its mechanism
	Effect of self-made Tiaoqi Decoction on cisplatin induced renal injury in rats and its mechanism[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(05): 396-400>')" href="#">	LI Xiaolan1， WANG Shaojun1， MA Yuefei1， WANG Linlin2
		Objective: To investigate effect of selfmade Tiaoqi Decoction on cisplatin induced renal injury in rats and its potential mechanism. Methods: Ninety male SpragueDawley rats were randomly divided into normal control group, model group, low, medium and high dose groups of Tiaoqi Decoction, and positive control group （n=15 for each group）. Cisplatin （2.5 mg/kg） was injected via the rat′s tail vein to induce kidney injury. The low, medium and high dose groups of Tiaoqi Decoction were administrated to rats at the doses of 2, 4, and 8 mL/kg by gavage. The positive control group was intraperitoneally injected with amifostine at a dose of 1 mg/kg, and the control group and model group rats were administrated with the normal saline （2 mL/kg） by gavage once a day for 60 consecutive days. The contents of serum urea nitrogen and creatinine were determined by colorimetry. The contents of tumor necrosis factorα （TNFα）, IL-6 and IL-1β of the kidney tissue, and the contents of TNF-α, IL-17 and matrix metalloproteinase-9 （MMP-9） secreted by spleen lymphocytes were determined by ELISA. The activity of superoxide dismutase （SOD） and the level of malondialdehyde were determined by colorimetric method and colorimetric method, respectively. Pathological changes of rat kidney tissue were examined by HE staining. The protein levels of Tolllike receptor 4 （TLR4） and NFκB in rat kidney tissue were tested by Western blotting. Results: Compared with the model group, urea nitrogen and creatinine levels of the serum, TNF-α, IL-6, IL-1β and MDA levels, TLR4 and NFκB protein levels of the kidney tissue, TNF-α, IL-17, MMP-9 levels of the spleen were significantly decreased in the low, medium and high dose groups of Tiaoqi Decoction （P<0.05）, but SOD activity was significantly increased（P<0.05）. The pathological changes of the kidney tissue was significantly attenuated. With the dose of Tiaoqi Decoction increasing, dosedependent changes of the indicators above were observed. Conclusion: Selfmade Tiaoqi Decoction could significantly attenuate renal injury induced by cisplatin in rats by inhibiting TLR4/NFκB pathway.
		2021 Vol. 31 (05): 396-400 [Abstract] ( 43 ) [HTML 1KB] [ PDF 10933KB] ( 369 )

	401	Detection of human parechovirus in neonates and analysis of genetic characteristics
	Detection of human parechovirus in neonates and analysis of genetic characteristics[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(05): 401-405，411>')" href="#">	LI Hong， TANG Wei， LV Jinquan
		Objective: To estimate the prevalence of human parechovirus （HPeV） in newborns via detection of HPeV in blood, fecal and nasopharyngel secretions. Methods: A total of 285 samples were collected， including 95 of blood， feces and nasopharyngeal secretions samples， respectively. Five libraries were constructed from 45 neonatal fecal samples and highthroughput sequencing was performed to detect HPeV by viral metagenomics. The whole genome was obtained by mapping with Geneious software， and the genetic characteristics of the strain were analyzed.The primers of nest polymerase chain reaction（PCR）were designed according to the capsid protein sequences of HPeV. A total of 285 samples were screened for HPeV by nestPCR. Results: A total of 248 534， 277 432， 398 756， 475 408 and 130 634 raw reads were generated for the 5 pools， and raw reads have been uploaded to NCBI′s Sequence Read Archive （SRA） database.A complete genome of HPeV was obtained from library newbornfeces41， and it was identified as HPeV1.Recombinant analysis showed that the strain was produced by HPeV-1 and HPeV-5 recombination， named newborn_zjlh. Six fecal samples were positive for HPeV screening; blood and nasopharyngeal secretions samples were negative. Conclusion: HPeV can be detected and maybe prevalent in newborns and mainly infects the intestinal tract.
		2021 Vol. 31 (05): 401-405，411 [Abstract] ( 75 ) [HTML 1KB] [ PDF 6242KB] ( 448 )

	406	Effects of bone marrow mesenchymal stem cells infusion on CXCR4 expression in B cells of MRL/lpr lupus mice
	Effects of bone marrow mesenchymal stem cells infusion on CXCR4 expression in B cells of MRL/lpr lupus mice[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(05): 406-411>')" href="#">	MA Cui， TANG Yu， CHENG Mengwei， SHI Wei， ZHENG Wenjuan，QIU Yingying， RUI Jinbing， WANG Yanru
		Objective： To study the effects of bone marrow mesenchymal stem cells （BMMSCs） infusion on plasma C3 and IgG concentrations， and CXCR4 expression of B cells and plasma cells in MRL/lpr lupus mice， and to explore the mechanism of BMMSCs in regulating disease activity of systemic lupus erythematosus. Methods： BMMSCs were isolated from C57BL/6 mice and cultured， and then injected into MRL/lpr lupus mice by tail transfusion. All mice were divided into C57BL/6 group （C57BL/6 mice were not injected ）， MRL/lpr group （MRL/lpr mice were not injected with BMMSCs）， BMMSCs infusion group （MRL/lpr mice were injected with BMMSCs at 12 weeks）， and PBS control group （MRL/ lpr mice were injected with PBS at 12 weeks）.At the end of the 20th week， peripheral blood， bone marrow and spleen of mice were taken for flow cytometry analysis to detect CXCR4 expression in B and plasma cells， and then the plasma of the 4 groups were taken for ELISA to detect IgG and C3 level. The kidney of mice was stained with hematoxylineosin. Results： ① Compared with the C57BL/6 group， the plasma C3 level were decreased and IgG level were significantly increased in the MRL/lpr group（all P<0.01）. Compared with C57BL/6 group， the expression of CXCR4 on B cell surface in MRL/lpr group was significantly decreased in peripheral blood， and was significantly increased in bone marrow and spleen（all P<0.01）. The proportion of CXCR4 on plasma cell surface in bone marrow（P<0.05） and spleen（P<0.01） of MRL/lpr group was significantly higher than that of C57BL/6 mice.② Compared with PBS or MRL/lpr group， the level of C3 in BMMSCs group was significantly increased， while the level of IgG was significantly decreased（all P<0.01）. Compared with the PBS group， the expression of CXCR4 on B cell surface of BMMSCs group was significantly increased in peripheral blood（P<0.01）， and significantly decreased in bone marrow（P<0.01） and spleen（P<0.05）. Compared with the MRL/lpr group， the expression of CXCR4 on B cell surface of bone marrow and spleen was significantly decreased （all P<0.01）.③ Compared with PBS group or MRL/lpr group， the expression of CXCR4 on plasma cell surface in bone marrow and spleen of BMMSCs group was significantly decreased（all P<0.05）.④ Renal HE staining suggested attenuation of renal inflammation in MRL/lpr mice after infusion of BMMSCs. Conclusion： BMMSCs infusion can regulate the expression of CXCR4 on the surface of B and plasma cells and improve the disease activity index of MRL/lpr mice.
		2021 Vol. 31 (05): 406-411 [Abstract] ( 40 ) [HTML 1KB] [ PDF 1715KB] ( 448 )

	412	Human esophageal cancer cells derived exosomes enhance the angiogenesis of human umbilical vein endothelial cells
	Human esophageal cancer cells derived exosomes enhance the angiogenesis of human umbilical vein endothelial cells[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(05): 412-416，420>')" href="#">	SHI Jinsheng, WANG Maosong, LI Benzhong, ZHANG Xiumei
		Objective: To explore the promotion effects of exosome from human esophageal cancer cells EC109 （EC109-ex） on the tube formation of human umbilical vein endothelial cells （HUVECs）. Methods: EC109ex was isolated from the supernatant of cultured EC109. EC109ex was characterized in terms of surface markers CD9 and CD63 by Western blotting and morphology by transmission electron microscopes （TEM）. HUVECs were treated with EC109ex （5 μg/mL and 10 μg/mL） or PBS （control group）, respectively. Then invasion and tube formation of HUVECs were examined using Transwell and Matrigel assay. Expression of vascular specific marker CD31, CD34 and VEGF were examined by Western blotting．Results: EC109ex located in the cytoplasm of HUVECs. Compared with control group，EC109-ex increased the migration efficiency (P<0.01) and number of tube formation of HUVECs （P<0.01）．Furthermore, EC109ex treatment increased expression of vascular specic markers of CD31, CD34 and VEGF in HUVECs（P<0.01）. Conclusion: Esophageal cancer cells could promote tumor angiogenesis through exosome mediated paracrine action.
		2021 Vol. 31 (05): 412-416，420 [Abstract] ( 45 ) [HTML 1KB] [ PDF 11623KB] ( 433 )

	417	Application of thoracoscopic paravertebral block in analgesia after thoracoscopic lobectomy
	Application of thoracoscopic paravertebral block in analgesia after thoracoscopic lobectomy[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(05): 417-420>')" href="#">	KE Pinhui, LIN Liangqing, WU Qinghua, YU Yaohua
		Objective: To evaluate the analgesic effects of thoracoscopic paravertebral block ,which used videoassisted thoracic for analgesia after thoracoscopic lobectomy. Methods: A total of 80 patients, who were treated by thoracoscopic lobectomy in Putian First Hospital from May to December 2019, and as American Society of anesthesiologists （ASA） class Ⅱ-Ⅲ were enrolled in the study. They were divided into control group and paravertebral block （PVB） group randomly and equally, 40 patients, using a random number table. Patients in control group were given patientcontrolled intravenous analgesia （PCIA） after operation. Patients of PVB group anesthetics were injected into the thoracic 4-5 and 8-9 intercostal at the end of surgery combined with PCIA after operation. Postoperative visual analogue scale （VAS） score, the times pressing analgesia pump button, the satisfactory of analgesia, the needs for rescue analgesia were recorded. In addition, the occurence of nausea, vomiting and dizziness and delirium were recorded after surgery. Results: Compared with control group, the VAS score of PVB group were significantly reduced, the times pressing analgesia pump button were decreased, the needs for rescue analgesia were also dropped, the satisfactory of analgesia were increased. Moreover, the incidence of postoperative nausea, vomiting and dizziness in PVB group were significantly lower than that in control group. Conclusion: Thoracoscopic paravertebral block, using videoassisted thoracic combined with PCIA after surgery can effectively alleviate the postoperative pain of thoracic surgey, reduce the reuse of analegsics and the incidence of postoperative adverse reactions.
		2021 Vol. 31 (05): 417-420 [Abstract] ( 44 ) [HTML 1KB] [ PDF 744KB] ( 505 )

	421	The correlation between serum lipoprotein（a） and risk of Alzheimer′s disease
		JIANG Fuping1， CHEN Nihong2， ZHU Lin2
		Objective： To investigate the correlation between serum lipoprotein（a）［Lp（a）］and Alzheimer′s disease（AD）.Methods： A retrospective casecontrol study was conducted on 103 AD patients>60 years old who were admitted to the Department of Neurology of Nanjing First Hospital from January 2015 to December 2018， and another 103 age and gendermatched healthy elderly subjects during the same period were selected as controls. Univariate and multivariate binary Logistic regression analysis and ROC curve were used to explore the correlation between serum Lp（a）level and the risk of AD. Furthermore， the effect of serum Lp（a） level on the risk of AD in different age groups was further investigated. Results： After the quinquepartite method of serum Lp（a） level， the first group（Q1）was used as a reference. With the increase of serum Lp（a） level， the risk of AD gradually decreased. The relative risks of groups Q2 to Q5 were 0.600， 0.471， 0.406 and 0.332（P for trend=0.011）， respectively. After adjusting for hypertension， diabetes， cardiovascular disease， serum creatinine， triglyceride， total cholesterol， highdensity lipoprotein cholesterol（HDLC）， glycosylated hemoglobin（HbA1c）， and lipoprotein associated phospholipase A2（LpPLA2）， Q1 was also used as a reference for estimating the relative risk of AD. The relative risks from Q2 to Q5 were 0.475， 0.343， 0.302 and 0.367（P for trend=0.020）， respectively. ROC curve analysis showed that the increase of serum Lp（a）level could reduce the risk of AD to a certain extent（AUC=0.601）， and it was not a good predictor. Taking lgLp（a） as the continuous variable， the increase of serum Lp（a） level was not correlated with the risk of AD in patients<75 years old（OR=1.082， P=0.769）， while the increase of serum Lp（a）level in patients≥75 years old was significantly correlated with the risk reduction of AD（OR=0.718， P=0.006）. Conclusion： There is a significant correlation between the increase of serum Lp（a）level in patients≥75 years old and the decrease of the risk of AD.
		2021 Vol. 31 (05): 421-425 [Abstract] ( 40 ) [HTML 1KB] [ PDF 992KB] ( 467 )

	426	Anti-convulsant mechanisms of Bombyx Batryticatus based on molecular docking and network pharmacology
	Anti-convulsant mechanisms of Bombyx Batryticatus based on molecular docking and network pharmacology[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(05): 426-430，437>')" href="#">	WANG Qiaoyu1, LIU Ying2, SHEN Jintian1, WANG Kaiyuan1,MEI Rong1, TANG Jian3, ZHANG Linsong2, WEN Chongwei1
		Objective: To explore the possible molecular mechanism of the anti-convulsant effect of Bombyx Batryticatus by network pharmacological analysis and molecular docking technology. Methods: Based on literature mining and database search, the chemical components of Bombyx Batryticatus were obtained, Bombyx Batryticatus targets were predicted using the Swiss Target Prediction database. DisGeNET database was used to collect convulsionrelated genes. The relationship network of Drug-Compounds-Target was constructed through Cytoscape software, and used STRING database to construct a protein interaction network. DAVID database was used for GO and KEGG analysis of the targets, and the components and targets were molecularly docked through Auto dock. Results: Through literature mining and database searching, a total of 18 potential active components and 513 effective targets of Bombyx Batryticatus were retrieved, and 22 targets of that intersect with convulsive diseases, these targets played a role in biological processes such as the regulation of cell processes, cell transport activity and receptor activity on the cell membrane, and is mainly involved in neuroactive ligandreceptor interaction, retrograde endocannabinoid signaling, cholinergic synapse and other 9 signal pathways, molecular docking technology verified that the components of Bombyx Batryticatus had good binding activity with potential targets. Conclusion: The “Drug-Active CompoundsTarget” network of Bombyx Batryticatus was constructed and may provide a theoretical reference for the systematic elucidation of the molecular mechanism of Bombyx Batryticatus anticonvulsant effect, as well as new ideas and methods for the development of new drugs.
		2021 Vol. 31 (05): 426-430，437 [Abstract] ( 55 ) [HTML 1KB] [ PDF 6429KB] ( 529 )

	431	Inhibition of Hepatitis B virus in liver cancer cells by preliminary peptide extracts of scorpion and centipede
	Inhibition of Hepatitis B virus in liver cancer cells by preliminary peptide extracts of scorpion and centipede[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(05): 431-437>')" href="#">	MA Qing1， YANG Yang2, MA Tao3, XU Weidong1, ZHAO Ming1
		Objective: To explore the effects of scorpion peptide preliminary extract （peptide extract from scorpion tail, PEST） and the centipede preliminary peptide extract （peptide extract from centipede head, PECH） on Hepatitis B virus synthesis and potential mechanism in liver cancer cells. Methods: Ultrafiltration was used to extract PEST and PECH. HepAD38 and HepG2 cells were selected and divided into control group, cells were cultured with high sugar medium containing with 10% fetal bovine serum; PEST group, cells were treated with different concentrations （0.125, 0.250, 0.500 and 1.000 mg/mL） of PEST; PECH group, cells were treated with different concentrations （0.125, 0.250, 0.500，1.000 mg/mL） of PECH; positive control group, cells were treated with lamivudine or entecavir or tetracycline; MTT assay was used to detect cell viability. The qRTPCR was used to detect Hepatitis B virus DNA content in the supernatant of HepAD38 cells. In addition, HepAD38 cells were divided into PEST group （cells were treated with 1 mg/mL PEST）, PECH group （cells were treated with 1 mg/mL PECH）, control group, positive control group and negative group; HBsAg and HBeAg content were detected by ELISA; HBV X，S，preC，P mRNA relative expression level were detected by qRTPCR; Hepatitis B virus core protein synthesis was detected by Western blotting. Results: The cell viability of HepG2 and HepAD38 cells treated with PEST or PECH were both above 70%；compared with the control group, PEST（0.250, 0.500 and 1.000 mg/mL） or PECH （0.125, 0.500 and 1.000 mg/mL） group showed significantly decreased Hepatitis B virus DNA copy number （all P<0.05）; compared with the control group, PEST or PECH group showed significantly lower content of HBsAg and HBeAg and expression level of Hepatitis B virus P mRNA （all P<0.05）, PEST group had almost no effect on core protein expression, while PECH group decreased Hepatitis B virus core protein expression. Conclusion: PEST and PECH exerted antiHepatitis B virus effect on HepAD38 cell, which may be related to its inhibition on P mRNA synthesis of Hepatitis B virus.
		2021 Vol. 31 (05): 431-437 [Abstract] ( 44 ) [HTML 1KB] [ PDF 1516KB] ( 513 )

	438


		2021 Vol. 31 (05): 438-442 [Abstract] ( 38 ) [HTML 1KB] [ PDF 1491KB] ( 695 )

	443


		2021 Vol. 31 (05): 443-447 [Abstract] ( 47 ) [HTML 1KB] [ PDF 992KB] ( 623 )

	448


		2021 Vol. 31 (05): 448-454 [Abstract] ( 35 ) [HTML 1KB] [ PDF 1431KB] ( 651 )

	455


		2021 Vol. 31 (05): 455-460 [Abstract] ( 35 ) [HTML 1KB] [ PDF 775KB] ( 446 )

江苏大学学报:医学版

　

News

　

　　　　　　　　　　More

　

Links

　

　　　　　　　　　　More

Copyright © 2011 Journal of Jiangsu University(Medicine Edition)
Editorial Department of Journal of Jiangsu University E-mail：xbyx@ujs.edu.cn