|
|
Anti-convulsant mechanisms of Bombyx Batryticatus based on molecular docking and network pharmacology |
WANG Qiaoyu1, LIU Ying2, SHEN Jintian1, WANG Kaiyuan1,MEI Rong1, TANG Jian3, ZHANG Linsong2, WEN Chongwei1 |
(1. School of Pharmacy, Jiangsu University, Zhenjiang Jiangsu 212013; 2. Zhenjiang Food and Drug Supervision and Inspection Center, Zhenjiang Jiangsu 212050; 3. School of Chinese Medicine, Bozhou University, Bozhou Anhui 236800, China)
|
|
|
Abstract Objective: To explore the possible molecular mechanism of the anti-convulsant effect of Bombyx Batryticatus by network pharmacological analysis and molecular docking technology. Methods: Based on literature mining and database search, the chemical components of Bombyx Batryticatus were obtained, Bombyx Batryticatus targets were predicted using the Swiss Target Prediction database. DisGeNET database was used to collect convulsionrelated genes. The relationship network of Drug-Compounds-Target was constructed through Cytoscape software, and used STRING database to construct a protein interaction network. DAVID database was used for GO and KEGG analysis of the targets, and the components and targets were molecularly docked through Auto dock. Results: Through literature mining and database searching, a total of 18 potential active components and 513 effective targets of Bombyx Batryticatus were retrieved, and 22 targets of that intersect with convulsive diseases, these targets played a role in biological processes such as the regulation of cell processes, cell transport activity and receptor activity on the cell membrane, and is mainly involved in neuroactive ligandreceptor interaction, retrograde endocannabinoid signaling, cholinergic synapse and other 9 signal pathways, molecular docking technology verified that the components of Bombyx Batryticatus had good binding activity with potential targets. Conclusion: The “Drug-Active CompoundsTarget” network of Bombyx Batryticatus was constructed and may provide a theoretical reference for the systematic elucidation of the molecular mechanism of Bombyx Batryticatus anticonvulsant effect, as well as new ideas and methods for the development of new drugs.
|
Received: 12 April 2021
|
|
|
|
[1]国家药典委员会. 中华人民共和国药典一部[S]. 北京: 中国医药科技出版社, 2015: 375.
[2]颜辉, 王国基, 王俊, 等. 僵蚕成分及药理作用研究进展[J]. 中国蚕业, 2004, 25(4): 86-88.
[3]程雪娇, 胡美变, 刘玉杰, 等. 僵蚕两种入药形式下的化学成分溶出性能与抗惊厥作用比较[J]. 中国药房, 2018, 29(9): 1242-1245.
[4]姚宏伟, 何欣暇, 何巧燕, 等. 僵蚕和蜈蚣醇提物抗惊厥作用的药效学比较研究[J]. 中国药物与临床, 2006, 6(3): 221-223.
[5]任小瑞,张恩欣,曾佳昕. 半夏天南星治疗肺癌机制的网络药理学研究[J].中药新药与临床药理, 2020,31(10): 1198-1206.
[6]张青, 徐月, 彭伟, 等. 分子对接结合网络药理学研究桂枝芍药知母汤治疗类风湿关节炎的分子作用机制[J]. 中草药, 2020, 51(18): 4673-4684.
[7]苏博. 264例小儿急诊惊厥病因分析[J]. 哈尔滨医药, 2013, 33(6): 432-433.
[8]翟淑萍, 余红辉, 卜简玲, 等. 小儿热惊厥对中枢神经系统的损伤[J]. 世界临床药物, 2008, 29(4): 249-251.
[9]高航, 张华北. 中枢神经系统α7烟碱型乙酰神经胆碱受体显像剂研究进展[J]. 同位素, 2019, 32(3): 218-230.
[10]Richer G. α7 Nicotinic acetylcholine receptor sand their role in cognition[J]. Brain Res Bull, 2013, 93: 86-96.
[11]刘孟. 与尼古丁成瘾相关的信号通路与蛋白质相互作用网络分析[D].天津:天津医科大学, 2015.
[12]Zhang CP, Zhu LL, Zhao T, et al. Characteristics of neural stem cells expanded in lowered oxygen and the potential role of hypoxiainducible factor1α[J]. NeuroSignals, 2006, 15(5): 259-265.
[13]Wakai T, Narasimhan P, Sakata H, et al. Hypoxic preconditioning enhances neural stem cell transplantation therapy after intracerebral hemorrhage in mice[J]. J Cereb Blood Flow Metab, 2016, 36(12): 2134-2145.
[14]贺锐, 杨红卫. 内源性大麻素与癫痫的研究进展[J]. 广东医学, 2014, 35(12): 1961-1963.
[15]Naidoo V, Karanian DA, Vadivel SK, et al. Equipotent inhibition of fatty acid amide hydrolase and monoacylglycerol lipasedual targets of the endocannabinoid system to protect against seizure pathology[J]. Neurotherapeutics, 2012, 9(4): 801-813.
|
|
|
|