● Article
JING Yang1, ZHENG Guo-xi2, ZHU Kang2, LIU Hui1, ZHANG Wen1
2015, 25(05): 369-372.
Objective: To observe the effect of rAAVNT4ADNF9 in treating ototoxicity associated with aminoglycoside antibiotics. Methods: The guinea pigs were used,and its auvicle reflex and auditory brainstem response(ABR) were normal.The ototoxic animal model of guinea pigs were made by the method of hypodemic injection of Kanamycin(400 mg/kg).In animals under general anesthesia,the guinea pigs were cut off the skin of ringt ear sulcus posterior and opened acoustic capsule,then injected the rAAVNT4ADNF9 10 μL from fossula rotunda.After 14 days,the right acoustic capsule were dislodged. The cochlear were stained with AgNO3.The cochlear hair cells were observed by contrast phase microscope. Results: Taking the group for rAAVNT4ADNF9 as example, the threshold ABR decreased remarkably after treatment(P＜0.05). Through the phase contrast microscope the cochlear basilar membrane was complete and the outer hair cells had dot deletion. The thresholds of ABR had no notable differences for the other three groups before and after treatment. Taking comparison between the artificial endolymph control group and empty vector group, through the phase contrast microscope after treatment, hair cells showed a patchy loss in the former group and there was no such phenomenon in the latter group. The average number of hair cells observed in the group of ADNF was significantly higher than that of the artificial endolymph control group and empty vector group(P<0.05). Conclusion: rAAVNT4ADNF9 is able to be transfected into cochlear,and to express secretory NT4ADNF9 peptide,which preventing hair cells from impairment.
