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  • Journal of Jiangsu University(Medicine Edition). 2024, 34(04): 354.
    Lon蛋白酶是一种存在于细胞中的ATP依赖型蛋白酶,主要负责细胞内异常蛋白质的催化分解,以维持生物体内蛋白质的质量水平。Lon广泛参与细胞凋亡、细胞分化和DNA修复等细胞活动。近年来研究发现,哺乳动物细胞中的线粒体Lon(LONP1)异常表达与一系列疾病的发生发展相关,包括线粒体缺陷相关疾病、神经退行性疾病以及癌症等。鉴于LONP1活性的检测与调控对相关疾病的早期诊断和治疗的重要性,本文针对LONP1结构、功能、与相关疾病的关系、活性调控及检测方法作一系统综述。
  • Journal of Jiangsu University(Medicine Edition). 2024, 34(06): 485-489.
    目的: 利用网络药理学和分子对接技术分析“茵陈-玉竹”药对治疗糖尿病肾病的机制。方法: 通过中药系统药理学数据库及分析平台(TCMSP)、中医药百科全书(ETCM)、中医药分子机制生物信息学注释数据库(BATMAN-TCM)检索“茵陈-玉竹”药对的主要化学成分,通过药物靶点数据库(TTD)、人类在线孟德尔遗传(OMIM)数据库、DisGeNET、GeneCards等数据库收集糖尿病肾病的疾病靶点,进而筛选茵陈、玉竹与糖尿病肾病的交集核心靶点。利用Cytoscape 3.7.2软件构建“化合物-靶点-疾病网络”,同时基于DAVID数据库对靶点进行GO功能富集和KEGG通路富集分析。结果: 获取“茵陈-玉竹”药对主要活性成分31个,作用靶点351个,糖尿病肾病相关基因2 911个,交集基因185个,其中起关键作用的活性成分为槲皮素、滨蒿内酯、莨菪亭、D-香芹酮和异鼠李素;构建可视化PPI网络得到185个节点,851条边,平均度值9.2,获得前列腺素内过氧化物酶2(PTGS2)、核因子κB1(NFKB1)、丝裂原活化蛋白激酶1(MAPK1)、MAPK3、MAPK14等“茵陈-玉竹”药对治疗糖尿病肾病的关键靶点。GO富集分析获得1 181条相关通路,KEGG通路富集获得180条信号通路。结论: “茵陈-玉竹”药对可能通过调控磷脂酰肌醇3激酶苏氨酸蛋白激酶(PI3K-Akt)、MAPK、糖基化终末产物糖基化终产物受体(AGE-RAGE)等信号通路,抑制各种炎症反应与氧化应激,调节异常代谢,从而对糖尿病肾病产生治疗作用。
  • Journal of Jiangsu University(Medicine Edition). 2024, 34(04): 317.
    目的: 观察经尿道输尿管软镜(flexible ureterorenoscopy,F-URS)联合可弯曲负压吸引输尿管导引鞘(简称负压吸引鞘)治疗复杂性肾结石的安全性和有效性。方法: 回顾性分析2021年6月至2023年6月联勤保障部队第906医院收治的456例复杂性肾结石患者的临床资料。根据手术方法不同分为负压组(n=230)和常规组(n=226)。负压组采用经尿道FURS联合负压吸引鞘治疗,常规组采用经尿道FURS钬激光碎石术治疗。比较两组的手术时间、结石清除率、住院时间、围术期并发症等情况。结果: 456例患者均一期完成手术。负压组230例中,200例顺利置入负压吸引鞘并行F-URS钬激光碎石术,5例输尿管扭曲狭窄改行微创经皮肾镜取石术(mini-percutaneous nephrolithotomy,M-PNL),25例输尿管狭窄改行支架管置入术,待后二期FURS。常规组226例中,188例顺利行F-URS,8例输尿管扭曲狭窄改行MPNL,30例输尿管狭窄改行支架管置入术,待后二期FURS。负压组平均手术时间(71.60±15.60)min,明显少于常规组(102.23±2915)min,差异有统计学意义(P<0.01);负压组结石清除率为82.50%(165/200),明显高于常规组69.15%(130/188),差异有统计学意义(P<0.01);负压组围术期尿源性感染率为8.50%(17/200),明显低于常规组25.53%(48/188),差异有统计学意义(P<0.05);两组患者平均住院天数无统计学差异(P>0.05)。结论: 与常规经尿道FURS钬激光碎石术对比,经尿道F-URS联合负压吸引鞘治疗复杂性肾结石能缩短手术时间,提高结石清除率,减少手术并发症。
  • Journal of Jiangsu University(Medicine Edition). 2024, 34(06): 461-468.
    Objective: To explore the hub genes and potential mechanism of ferroptosis in the development of atherosclerosis (AS) in patients with type 2 diabetes mellitus (T2DM) based on bioinformatics. Methods: The datasets GSE20966 (T2DM) and GSE43292 (AS) were obtained from the GEO database. Differentially expressed genes (DEGs) were identified using the Limma R package. Heatmaps and volcano plots were drawn, and crossanalysis was performed to obtain DEGs associated with the two diseases. GO and KEGG enrichment analysis was performed to explore the biological functions of DEGs. Ferroptosis-related genes (FRGs) obtained from the FerrDb database were crosslinked, and hub genes were screened using LASSO regression and random forest analysis. ROC curves and validation sets GSE76895 (T2DM) and GSE28829 (AS) were used for verification. Finally, the gene-miRNA network was drawn. Results: A total of 606 DEGs were identified related to the T2DM and the AS datasets. Twenty potential genes were obtained by cross-analyzing with FRGs. Cyclin-depedent kinase inhibitors 1A (CDKN1A), poly ADPribose polymerase 8 (PARP8), phosphatidylethanolamine binding protein 1 (PEBP1), and progesterone receptor membrane component 1 (PGRMC1) were hub genes that affected AS in T2DM patients through ferroptosis. The area under the curve (AUC) of the ROC curves in the datasets GSE20966 and GSE43292 of the four genes was all greater than 0.7, which had diagnostic value. PEBP1 and PGRMC1 were significantly down-regulated in the validation sets GSE76895 and GSE28829. In addition, 13 miRNAs were closely associated with 4 hub genes. Conclusion: CDKN1A, PARP8, PEBP1 and PGRMC1 are involved in AS in T2DM patients through ferroptosis and may become new therapeutic targets.
    Key words]ferroptosis; type 2 diabetes mellitus; atherosclerosis; bioinformatics; machine learning
  • Journal of Jiangsu University(Medicine Edition). 2024, 34(06): 476-484.
    Objective: To explore the effect of CXCL14 on pyroptosis of adipocytes and the formation of atherosclerosis in diabetic microenvironment. Methods: ① ApoE-/- mice were intraperitoneally injected with streptozotocin to construct diabetes mellitus, and diabetic ApoE-/- mice were fed with high fat diet for 20 weeks, and the diabetic atherosclerosis model (model group) was constructed, and control mice were only fed on a high-fat diet (AS group); ② Anti-CXCL14 short peptide was injected subcutaneously on the medial hind limb of diabetic mice (anti-CXCL14 group), and control diabetic mice were injected subcutaneously with normal saline only; ③ Adeno-associated virus (AAV) was injected in situ into posterior inguinal subcutaneous adipose tissue in diabetic mice to inhibit gastrointestinal dermatin (GSDMD)mediated pyroptosis, divided into: AAV-shscramble group, AAV-shGSDMD group, anti-CXCL14+AAV-shscramble group, anti-CXCL14+AAV-shGSDMDgroup. After 20 weeks of high-fat diet of the mice, the serum of the mice was collected under anesthesia, and the blood lipid levels (total cholesterol, triglycerides, LDL-C, and HDL-C) of the mice were analyzed by biochemical detection kit. The mice were sacrificed, and the epididymal adipose tissue was scanned by electron microscopy to observe the changes of fat cells. qRT-PCR was used to analyze the changes of pyroptosisrelated factors GSDMD, aspartate proteolytic enzyme-1 (Caspase-1), NLR family pyridine domain protein 3 (NLRP3) inflammasome, interleukin-1β (IL-1β) and inflammatory factor interleukin-6 (IL-6). The mouse aorta was isolated and extracted, and the relative area of atherosclerotic plaques was observed by HE staining and oil red O staining. Results: Compared with the AS group, the hypertrophy and number of adipocytes of adipose tissue in the epididymis in the model group decreased (P<0.01), the size of aorticplaque increased, the levels of total cholesterol, triacylglycerol and LDL-C were significantly increased, and HDL-C was significantly reduced. The levels of pyroptosisrelated factors and IL-6 in adipose tissue of epididymis were significantly increased (P<0.05), indicating that diabetes promoted pyroptosis of AS plaque and adipose tissue in mice. Injection of anti-CXCL14 short peptide could reduce the size of aortic plaque, improve blood lipid levels, and inhibit adipose tissue pyroptosis, which increases the number of fat cells. After GSDMD knockdown, the number of adipocytes increased and the area of aortic plaques decreased. However, after the injection of anti-CXCL14 immune peptide, there was no significant change in atherosclerosis in the AAV-shGSDMDgroup. Conclusion: Anti-CXCL14 can attenuate adipose tissue pyroptosis and alleviate the development of diabetic atherosclerosis.
    Key words]CXCL14; pyroptosis; adipose tissue; atherosclerosis; diabetic microenvironment
  • LU Xiaoping, CAI Xu, CHEN Xinpeng, HONG Yiwei, XIAO Jianwei
    Journal of Jiangsu University(Medicine Edition). 2024, 34(05): 377-382.
     Objective: To develop an effective and straightforward tool for assessing the risk of osteoporosis (OP) development in patients with rheumatoid arthritis (RA), thus early intervention can be facilitated to enhance patient prognosis and quality of life. Methods: A retrospective study was conducted, selecting 53 RA patients and 44 RA patients with concurrent OP treated at our institution from January 2018 to June 2023. Twenty-four predictive factors were collected. Key predictors were identified using the Lasso,Boruta and SVM-REF algorithms, and a predictive model was established using multivariate Logistic regression. Further validation of the model was performed using KNN and Lightgbm algorithms. Results: Four critical predictive factors were identified: IL-4, TT4, Anti-CCP, and age. The developed clinical prediction model exhibited a C-index of 0.82 and an area under the ROC curve (AUC) of 0.821. Clinical decision curve analysis indicated the highest net clinical benefit when the threshold probability ranged from 002 to 090, demonstrating the model′s robust predictive capability. Machine learning validation revealed that the KNN and Lightgbm models had AUCs of 0.973 and 0.973, respectively, and areas under the PR curve of 0.974 and 0.969, respectively. Confusion matrix results showed the KNN prediction model had a sensitivity of 0.866, specificity of 0.962, accuracy of 0.928, and F1 score of 0.918; the Lightgbm prediction model had a sensitivity of 0.955, specificity of 0.925, accuracy of 0.938, and F1 score of 0.933. Conclusion: This study successfully constructed a clinical prediction model for RA patients at risk of developing OP, and age, Anti-CCP, and TT4 was identified as high-risk factors, while IL-4 as a protective factor.
     
  • Journal of Jiangsu University(Medicine Edition). 2024, 34(03): 271-276.
           外泌体是含有来源细胞内容物的细胞外小囊泡,通过传递蛋白、核酸及脂类等生物活性分子介导细胞间通讯。肿瘤来源的外泌体与肿瘤的耐药性密切相关,环状RNA(circular RNA,circRNA)是一种环形稳定的内源性非编码RNA。近年来,关于外泌体转移circRNA在多种肿瘤耐药发展中的作用研究取得进展。因此,本文总结了外泌体circRNA参与肿瘤发病和耐药机制的研究内容。
  • Journal of Jiangsu University(Medicine Edition). 2024, 34(06): 469-475.
    Objective: To investigate the effect of human umbilical cord mesenchymal stem cell derived small extracellular vesicles (hucMSC-sEVs) in attenuating the damage of diabetic kidney disease (DKD) in rats. Methods: HucMSCs were isolated and cultured, and hucMSC-sEVs were extracted from the culture media supernatant. The DKD rat model was established by high fat diet combined with injection of streptozocin, rats were randomly divided into control group, DKD group, and hucMSCsEVs group, with 6 rats in each group. The hucMSC-sEVs group received tail vein injection of hucMSC-sEVs 8 weeks after modeling, and kidney tissues were collected at week 24. Renal tissue pathology and fibrotic changes in rats were assessed using HE staining, PAS staining and Sirius Red staining. The expression of apoptosisrelated proteins Bcl-2 and Bax in kidney tissues was evaluated using Western bloting. Additionally, the expression levels of THBS1 and its receptors CD36 and CD47 in rat renal tissues were determined using histochemical staining, immunofluorescence staining, and Western bloting. Results: Compared with the control group, the DKD group exhibited significant pathological changes in the kidneys, such as thickening of the glomerular basement membrane and mesangial proliferation, dilation of renal tubules accompanied by vacuolar degeneration, and marked interstitial fibrosis with infiltration of inflammatory cells. The expression of the apoptosis marker Bax in kidney tissues showed a significant increase (P<0.05), while the expression of the anti-apoptotic marker Bcl-2 exhibited a significant decrease (P<0.01). The expression levels of THBS1 and its receptors CD36 and CD47 were also significantly elevated (P<0.001). In contrast, compared with the DKD group, the hucMSC-sEVs group demonstrated marked alleviation of renal tissue pathological damage and fibrosis. The expression of Bax significantly decreased (P<0.01), while the expression of Bcl-2 significantly increased (P<0.01). Additionally, the expression levels of THBS1 and its receptors CD36 and CD47 in the kidneys were significantly reduced (P<0.001). Conclusion: HucMSC-sEVs can significantly reduce the renal tissue injury in DKD, which may be related to the targeted inhibition of THBS1 expression.
    Key words]human umbilical cord mesenchymal stem cells; small extracellular vesicles; diabetic kidney disease; THBS1
  • Journal of Jiangsu University(Medicine Edition). 2024, 34(04): 297.
    脓毒症是可导致机体严重器官功能障碍、危及生命的炎性综合反应,一般是由病原微生物侵入机体引发,近年来发生率和病死率居高不下,是重症监护病房患者死亡的重要原因。细胞焦亡是新发现的一种伴随炎症因子释放的细胞程序性死亡,可一定程度上抵御微生物的感染,但过度激活会诱导和加重脓毒症。目前有研究证明,细胞焦亡的许多相关分子都参与了脓毒症的发病进程,并在其中发挥重要作用。对脓毒症中细胞焦亡的研究不仅可深入阐明细胞焦亡的病理作用和机制,也有助于对脓毒症的发生发展和转归预判,尤其为发现治疗脓毒症的靶点拓展新思路。
  • Journal of Jiangsu University(Medicine Edition). 2025, 35(01): 86-92.
    心血管疾病是目前临床上常见和致死致残率较高的疾病之一,而内皮细胞稳态在心血管健康的维持中发挥着不可忽视的作用。作为机体非循环细胞类型中数量最多的一类细胞,内皮细胞能够通过多种方式独立地影响周围组织。本文介绍了内皮细胞的功能、常见损伤机制及其与心血管其他组成细胞之间的相互作用,并进一步探讨内皮细胞在心血管疾病发生发展中的作用及基于内皮细胞的心血管疾病诊疗新进展,以期为通过干预内皮细胞改善心血管疾病预后提供新的视角。

  • Journal of Jiangsu University(Medicine Edition). 2024, 34(03): 203-210.
    随着人们生活质量的提高和饮食习惯的改变,高尿酸血症的发病率逐年增加,并且趋于年轻化,成为主要的代谢性疾病。目前临床上用于治疗高尿酸血症的药物种类有限,不良反应较多,难以达到当前高尿酸血症的治疗需求,亟待开发新型降尿酸药物满足临床迫切需要。近年来国内外展开了一系列新型降尿酸药物研发,其中包括化学药物和中药等。随着研究深入,以尿酸转运蛋白1(urate transporter 1,URAT1)为抑制靶点的新型降尿酸药物研发取得了一定成果并陆续应用于临床。本文就URAT1为抑制靶点的药物研究进展作一综述,以期为新型降尿酸药物的研究及临床运用提供参考。
  • Journal of Jiangsu University(Medicine Edition). 2024, 34(06): 490-496.
           指南对中医临床开展标准化诊疗具有里程碑的意义,糖尿病肾病作为糖尿病的常见并发症,当前缺少与之相关的全面、规范且标准化的中医诊疗指南。本研究通过对既往发布的糖尿病肾病中医诊疗指南进行梳理,分析指南内容并提出建议,以期今后制定糖尿病肾病的中医诊疗指南能进一步凸显中医特色、提升方法学质量,为临床实践提供更多参考依据。
  • Journal of Jiangsu University(Medicine Edition). 2024, 34(06): 542-549.
    Objective: To establish an albendazole-loaded self-microemulsifying drug delivery system (ABZ-SMEDDS) to improve the solubility and oral bioavailability of the water poorly soluble drug. Methods: The composition of the self-microemulsion prescription was optimized by the establishment of pseudo-triphasic diagrams, then the particle size, drug loading, encapsulation rate, and micromorphology were determined. The in vitro drug release and cytotoxicity and cellular uptake were investigated on a Caco-2 cell model, and finally, the oral bioavailability of ABZ-SMEDDS in rats was investigated. Results: The optimal prescription composition of ABZ-SMEDDS is clove oil, polyoxyethylene hydrogenated castor oil RH40 and polyethylene glycol 400 in a mass ratio of 0.20∶0.64∶0.16. The particle size of ABZ-SMEDDS was (52.14±1.82)nm with a polydispersity index of 0.084±0.006. The drug loading and encapsulation efficiency were (36.60±1.20)mg/g and (98.12±2.2)%, respectively. The results of in vitro release and cell experiments showed that the dissolution rate of ABZ-SMEDDS in different dissolution media were greatly improved compared with ABZ, and it can promote the transmembrane absorption of drugs. Pharmacokinetic results in vivo showed that the relative oral bioavailability of ABZ-SMEDDS in rats was increased to 151.95% compared with the free drug. Conclusion: SMEDDS could be a potential carrier for the enhancement of drug dissolution and oral bioavailability of poorly water soluble drugs.
    [Key words]albendazole; selfmicroemulsion; dissolution in vitro; cellular uptake; oral bioavailability
  • Journal of Jiangsu University(Medicine Edition). 2024, 34(03): 190-196.
    Objective: To explore the correlation between triglyceride glucose (TyG) index and hyperuricemia (HUA) in patients with systemic lupus erythematosus (SLE). Methods: A retrospective cross-sectional study was conducted on 557 hospitalized SLE patients who were initially treated at Nanjing Drum Tower Hospital. Based on the presence or absence of hyperuricemia,they were divided into the HUA group and the SLE group, and a comparative analysis was performed on clinical data, laboratory indicators, and medication usage between the two groups. Logistic regression, restricted cubic spline, and subgroup analysis were used to evaluate the association between TyG index and hyperuricemia. Additionally, the values of various insulin resistance (IR) metabolic indices in predicting the risk of hyperuricemia in SLE patients were also compared using the area under the receiver operating characteristic curve (ROC). Results: In the HUA group, body mass index, systolic blood pressure, diastolic blood pressure, TyG index, 24 hour urine protein, creatinine, the prevalence of combined hyperlipidemia, and the use of tacrolimus were significantly higher compared to the SLE group. After controlling for confounding factors, binary logistic regression analysis revealed that the risk of developing hyperuricemia in the T3 (≥8.82) group, based on TyG index tertile grouping, was 2.900 times higher than that in the T1 (<8.31) group (95%CI: 1.565-5.371, P<0.05). There was a positive linear correlation between TyG index and the risk of hyperuricemia in SLE patients (P for trend<0.05, P for nonlinearity>0.05). Subgroup analysis further suggested gender and age differences in the association between TyG index and hyperuricemia. ROC curve analysis showed that the area under the curve for predicting the occurrence of hyperuricemia in SLE patients using TyG index,TG/HDL-C ratio, METS-IR, and TyG-BMI were 0.651, 0.656, 0.629, and 0.612, respectively. TyG index and TG/HDL-C had higher predictive values for SLE patients with hyperuricemia. Conclusion: Elevated TyG index is associated with an increased risk of hyperuricemia in SLE patients. As a low-cost and readily available indicator, TyG index may be used to predict the risk of hyperuricemia in patients with SLE.
    Key words]triglyceride glucose index; insulin resistance; hyperuricemia; systemic lupus erythematosus
  • CHENG Wenxiao, WANG Deqiang, TANG Yu, MAO Chaoming
    Journal of Jiangsu University(Medicine Edition). 2025, 35(01): 39-45.
    Objective: To prepare a photothermal drug carrier capable of responding to the tumor environment, enabling controlled drug release within tumor tissues for precise targeting and destruction of tumor cells in vivo. Methods: Chemotherapeutic drug doxorubicin (DOX) was loaded into the surface of graphene oxide (GO) carriers to produce GO-DOX nanoparticles, and the change of DOX fluorescence signal was monitored. First, GO-DOX was respectively incubated with mouse breast cancer 4T1 cells and human gastric epithelial GES-1 cells, and the fluorescence intensity of the two cell types was observed at different time points by confocal microscope. Then, the tumor xenograft model of 4T1 mouse was established and the changes of tumor volume of mice were observed. Last, tumor tissues were collected to prepare pathological sections, and the inhibitory effect of GO-DOX nanoparticles combined with near infrared light on xenografts in mice was evaluated. Results: GO-DOX nanoparticles not only had the environmental responsiveness to control drug release, but also were used to fluorescently image tumor cells. The combination of GO-DOX nanoparticles and near-infrared light effectively inhibited tumor growth and reduced the toxic and side effects of DOX on human body. Conclusion: Environment-responsive GO-DOX nanoparticles were successfully prepared, and can be used to effectively inhibite the growth of tumor and realize real-time imaging of tumor cells.

  • Journal of Jiangsu University(Medicine Edition). 2025, 35(01): 1-4.
    江苏大学学报(医学版)2025年第1期目次
  • Journal of Jiangsu University(Medicine Edition). 2024, 34(03): 185-189,196.
    Objective: To investigate the effect of mesenchymal stem cells (MSC) transplantation on pristane induced pulmonary alveolar hemorrhage in lupus mice and explore the possible mechanism. Methods: Twenty female C57BL/6 (B6) mice at 10 weeks of age were injected with 0.5 mL pristane intraperitoneally and 3 control B6 mice were injected with the same volume of PBS. After one week, mice injected with pristane were randomly divided into two groups and intravenously injected with 1×106 human umbilical cord-derived MSCs or PBS, respectively. After another week of observation, the survival rate and body weight of the two groups were recorded and all mice were sacrificed. The appearance of the lungs was observed and hematoxylin and eosin (HE) staining was performed to assess the severity of pulmonary hemorrhage. Lung single-cell suspensions were prepared by enzymatic digestion. CD4+ and CD8+ T lymphocytes, CD19+ B lymphocytes, CD11b+Gr1+ neutrophils, F4/80+ macrophages and CD206+ macrophages in lungs were detected by flow cytometry. Results: The survival rate of mice in the MSC group was higher than that of the PBS group, but the difference was not statistically significant (P=0.07), and the average body weight in MSC mice was significantly increased (P<0.01) than that in PBS group. The incidences of complete diffuse alveolar hemorrhage and partial diffuse alveolar hemorrhage in the MSC group were lower than that in the PBS group. Compared with the PBS mice, the total number of lung cells in the MSC group was significantly reduced (P<0.01) than that in the PBS group. The number of lung CD4+ T cells in the MSC group showed a decreased tendency, while the numbers of CD8+ T cells and CD19+ B cells were significantly decreased (P<0.05) in the MSC group than that in the PBS group. The absolute numbers of CD11b+Gr1+ neutrophils and F4/80+ macrophages in lungs of MSC treated mice were also markedly reduced (P<0.01 and P<0.05). In addition, the percentage of CD206+ anti-inflammatory macrophages in lungs of pulmonary hemorrhage mice was significantly reduced (P<0.01), while the percentage of CD206+ anti-inflammatory macrophages was significantly increased by MSC transplantation (P<0.01). Conclusion: MSC transplantation can significantly reduce the incidence of pulmonary hemorrhage in lupus-prone mice, which may attributed to the regulation of lung immune cells and the induction of CD206 anti-inflammatory macrophages by MSCs.
    Key words]mesenchymal stem cell; systemic lupus erythematosus; alveolar hemorrhage; macrophage; CD206
  • KANG Yinlin, LI Yi, TANG Yunlin, et al.
    Journal of Jiangsu University(Medicine Edition). 2024, 34(04): 338.
    Objective: To investigate the clinical efficacy of metformin combined with orlistat in obese patients with polycystic ovary syndrome (PCOS) and insulin resistance. Methods: A total of 150 obese PCOS patients with insulin resistance were randomly divided into control group and research group, with 75 cases in each group. The control group was given basic treatment such as metformin and ethinylestradiol cycloproterone, and the research group was treated with basic treatment combined with orlistat. After 3 months of treatment, the differences in anthropometric indicators, glycolipid metabolism indicators and sex hormone levels between the two groups were compared. Results: After 3 months of treatment, compared with the control group, the body weight, body mass index (BMI), waist-to-hip ratio (WHR), body fat percentage, fasting plasma glucose (FPG), 2 h postprandial plasma glucose (2hPG), fasting insulin (FINS), glycosylated hemoglobin (HbA1c), and homeostasis model assessment-IR (HOMA-IR) of the research group were significantly decreased (P<0.05); and the triglyceride, total cholesterol, low density lipoprotein cholesterol in the research group were also significantly decreased (P<0.05), while high density lipoprotein cholesterol was significantly increased (P<0.05); the levels of luteinizing hormone (LH), estradiol, testosterone, prolactin and LH/follicle stimulating hormone (FSH) were greatly decreased (P<0.05), while there was no significant difference in FSH (P>0.05). Conclusion: Metformin combined with orlistat in the treatment of obese PCOS with insulin resistance could significantly improve the glucose and lipid metabolism, reduce weight and insulin resistance and improve sexual hormone disorder.
  • Journal of Jiangsu University(Medicine Edition). 2024, 34(06): 497-506.
    Objective: To explore intercellular communication-related signaling molecules in tumor microenvironment (TME) of castration-resistant prostate cancer (CRPC) and analyze their relationship with tumor occurrence and development. Methods: The main components of the TME in CRPC was analyzed using singlecell RNA sequencing (scRNA-seq) data (GSE137829) from CRPC patients found in the Gene Expression Omnibus (GEO) database. CellPhoneDB was used to examine interactions between different cells within the microenvironment, with a focus on revealing the connections between cancer-associated fibroblasts (CAFs) and malignant epithelial cells. CellChat was applied to analyze signaling molecules involved in communication between CAFs and malignant epithelial cells. Data from The Cancer Genome Atlas Prostate Adenocarcinoma (TCGA-PRAD) were integrated to identify signaling molecules closely related to the prognosis of CRPC patients. siRNA was used to knock down the expression of the signaling molecule Delta-like canonical Notch ligand 3 (DLL3) in CRPC cells, and the knockdown efficiency was validated by real-time quantitative PCR and Western blotting. The effects of DLL3 on the biological behaviors of CRPC cells, including proliferation, migration, and invasion, were investigated using CCK8 assays, EdU assays, and Transwell assays with Matrigel, respectively. Singlegene differential analysis, overrepresentation analysis (ORA), and gene set enrichment analysis (GSEA) were also performed to identify pathways enriched with DLL3-related differential expression genes. Results: The results of single-cell transcriptomic sequencing indicated that the main cellular components of the TME in CRPC include malignant epithelial cells, CAFs and immune cells. Close communication between CAFs and malignant epithelial cells was mediated by interaction-related signaling molecules, with 63 signaling molecules identified as closely related to the communication between CAFs and malignant epithelial cells. Univariate Cox regression analysis identified key signaling molecules, including tumor necrosis factorrelated ligand superfamily member 12 (TNFSF12) and DLL3, whose transcriptional expression were significantly associated with the prognosis of CRPC patients. Notably, high expression of DLL3 was significantly associated with poor prognosis in CRPC patients. In vitro experimental results demonstrated that knockdown of DLL3 significantly inhibited the growth, proliferation, migration and invasion capabilities of CRPC cells. KEGG and GSEA enrichment analyses indicated that differentially expressed genes related to DLL3 were significantly enriched in metabolic pathways. Conclusion: In the TME, CAFs and malignant epithelial cells communicate closely through intercellular signaling molecules. Among these signaling molecules, the expression of DLL3 is negatively correlated with cancer prognosis and affects the biological behavior of CRPC cells, suggesting that DLL3 may be a key signaling molecule in the malignant progression of human CRPC.
    Key words]castration-resistant prostate cancer; scRNA-seq; tumor microenvironment; cell communication; DLL3
  • Journal of Jiangsu University(Medicine Edition). 2024, 34(06): 536-541.
    Objective: To study the optimized formulation of nidanib ethanesulfonate (NE) solution for inhalation and evaluate the formulation in vitro and in vivo. Methods: The prescription of NE solution was studied by optimizing the amount of the antioxidants, osmotic pressure regulators and pH value. The dynamic particle size of NE nebulized inhalation formulations was measured. The in vivo pharmacokinetics of NE nebulized inhalation formulations was determined by high performance liquid chromatography. Lung function, hydroxyproline (HYP), tumor necrosis factor-α(TNF-α), transforming growth factor-β1 (TGF-β1) were used as indicators to evaluate the therapeutic effect in vivo. Results: Optimized prescription was obtained: NE: 10 mg, sodium bisulfite: 4 mg, propylene glycol: 50 mg, water: 2 mL. The pH of the solution was adjusted to 4.0. The average particle size of NE nebulized inhalation was about 3.3 μm. The in vivo pharmacokinetic results in SD rats showed that the elimination half-life of NE inhalation preparation was 5.27 h. The bioavailability was significantly improved, reaching 63.71%. Pulmonary function parameters (tracheal contraction parameters, 50% tidal volume expiratory flow rate, respiratory rate) and lung dry/wet weight ratio, HYP, TNF-α in mice with pulmonary fibrosis model after inhalation administration had been significantly improved. Conclusion: NE inhalation can greatly improve lung function parameters and increase its bioavailability.
    [Key words]nidanib ethanesulfonate; inhalation preparations; nebulized inhalation; pharmacokinetics; idiopathic pulmonary fibrosis
  • Journal of Jiangsu University(Medicine Edition). 2024, 34(06): 522-531.
    Objective: To construct a prognostic risk model for predicting bone metastasis in prostate cancer (PCa) based on bioinformatics approach, and to investigate the effect of risk score on prognosis and immune infiltration. Methods: Clinical information and microarray expression data of PCa patients were downloaded from the Gene Expression Omnibus (GEO), and genes related to the bone morphogenetic protein (BMP) pathway were downloaded from the Molecular Signature Database (MSigDB). The differential expression genes (DEGs) of PCa bone metastasis samples and primary PCa samples were screened by differential analysis, and the intersection with BMP pathway-related genes was taken to obtain BMP-related DEGs, and the prognostic risk model was constructed by screening risk genes through LASSO regression analysis, and independent prognostic factors of PCa were screened by multifactorial regression analysis. The risk scores of patients were calculated according to the risk model and subsequently divided into two groups of high and low risk scores by corresponding score median values, and DEGs were identified for functional enrichment analysis to compare the differences in immune infiltration between high and low risk groups. Results: After the intersection of DEGs and BMP-related gene sets in 3 055 differentially expressed genes of PCa bone metastasis samples, 13 BMP-related DEGs were obtained. Four gene were screened by LASSO regression to construct a prognostic risk model, in which secreted frizzled-related proteins 2 (SFRP2), endoglin (ENG), and follistatin-like protein 1 (FSTL1) were risk genes and chordin like 1 (CHRDL1) was a protective gene. Kaplan-Meier analysis showed that metastasis-free survival and biochemical recurrence-free survival of patients in the high-risk group were significantly lower than that of patients in the low-risk group. Multifactorial regression analysis identified risk score as an independent prognostic factor for PCa bone metastasis, and patients in the high-risk group were more likely to develop bone metastasis and biochemical recurrence, and the risk score was positively correlated with the PSA value, the Gleason score, and the T stage. The DEGs of high-risk group were mainly involved in the activation of the WNT/BMP signaling pathway, and were significantly enriched in the proliferation of epithelial cells and extracellular matrix bioprocesses. GSEA analysis showed that, the differentially expressed genes were upregulated in the gene sets of mesenchymal transition, inflammatory response, angiogenesis, and multiple tumor metastasis gene sets. Immune infiltration analysis showed that PCa bone metastasis samples and patients in the high-risk group possessed a higher degree of immune cell infiltration, cancer associated fibroblasts (CAFs), macrophages, Estimate scores, stromal scores, and immune scores than the lowrisk group, and lower tumor purity than the low-risk group. Conclusion: The prognostic risk model of BMP-related DEGs constructed based on the LASSO regression analysis can effectively predict the occurrence of PCa bone metastasis, and the high-risk score is closely related to the prognosis and immune infiltration of PCa.
    [Key words]prostate cancer; bone metastasis; BMP signaling pathway; prognostic risk model; immune invasion
  • SONG Jiajia, ZHANG Xuan, GAO Zihan, XU Wenrong, QIAN Hui, LIANG Zhaofeng
    Journal of Jiangsu University(Medicine Edition). 2025, 35(01): 52-55.
    Objective: Blueberry derived exosome-like nanoparticles (BELNs) were extracted and identified, and the intervention effect of BELNs on the malignant transformation of human normal gastric mucosal epithelial cells induced by (N-methyl-N′-nitro-N-nitrosoguallidine, MNNG) was investigated. Methods: The BELNs were extracted by ultracentrifugation, and the BELNs were identified with particle size, Zeta potential and morphology by nano-particle tracking analyzer and transmission electron microscopy. Laser confocal microscopy was used to observe whether BELNs could be endocytosed by transformed gastric mucosal epithelial cells (TGES-1). TGES-1 cells were treated with 200 and 400 μg/mL BELNs for 72 h, then Western blotting was used to analyze the expression of stemness genes, epithelialmesenchymal transition (EMT) and proliferation related proteins. Results: The results of nano-particle tracking analyzer and transmission electron microscopy showed that BELNs was matched with the characteristics of exosome-like nanoparticles. The results of laser confocal microscopy confirmed that BELNs could be endocytosed by TGES-1 cells. Western blotting showed that 400 μg/mL BELNs inhibited the expression of N-cadherin, E-cadherin, Vimentin, Nanog and proliferating cell nuclear antigen (PCNA) and enhanced expression of E-cadherin in TGES-1 cells. Conclusion: BELNs exert a significant intervention effect on human normal gastric mucosal epithelial cells induced by MNNG.

  • GU Liqin1, CHEN Xiaoyi2, ZHANG Yanju1, CHEN Xiaojun1
    Journal of Jiangsu University(Medicine Edition). 2025, 35(01): 56-61.
    Objective: To investigate the risk factors of hospital infection in stroke patients and construct a risk prediction model of nosocomial infection. Methods: A total of 300 stroke patients with nosocomial infection during hospitalization in the Department of Neurology and Neurosurgery of Affiliated Hospital of Nantong University from January 2020 to December 2023 were also selected (infection group), and another 300 stroke patients without nosocomial infection during the same period were selected (control group). The distribution of nosocomial infection and pathogenic bacteria were analyzed, and clinical characteristics of the two groups were compared. Multivariate Logistic regression was used to analyze the risk factors of nosocomial infection in stroke patients, and a nomogram model for predicting risk was established by incorporating R language, and the predictive effect of the model was evaluated. Results: The predominant nosocomial infection site of stroke patients was the respiratory system (62.67%, 188/300), the predominant pathogenic bacteria of nosocomial infection were Gram-negative bacteria (59.90%, 121/202), and the multidrug-resistant bacteria infection accouned for 34.16% (69/202). Compared with the control group, the infection group had higher proportion of cerebral hemorrhage, diabetes, hypertension, coronary heart disease, disturbance of consciousness, central venous catheters, ventilators, urinary catheters, and preventive use of antibiotics, body mass index(BMI)≥24 kg/m2 and hospitalization time≥14 d (P<0.05). Multivariate Logistic regression analysis showed that stroke type, hypertension, diabetes, BMI≥24 kg/m2, disturbance of consciousness, use of ventilator, indwelling urinary catheter, preventive use of antibiotics, and hospitalization time≥14 d were independent risk factors for nosocomial infection in stroke patients (P<0.05). A nomogram prediction model was constructed based on the regression results, and the area under ROC curve was 0.983 (95%CI: 0.975-0.991). The sensitivity and specificity was 0.940 and 0.937, respetictively. The Hosmer-Lemeshow test (χ2=5.454, P=0.708) indicated that the model had a good fit and predictive performance. Conclusion: The risk factors of nosocomial infection in stroke patients include stroke type, hypertension and diabetes, etc. The prediction model based on the risk factors could accurately predict the risk of nosocomial infection in stroke patients.

  • Journal of Jiangsu University(Medicine Edition). 2025, 35(01): 70-72.
    目的: 探讨健侧卧奔跑位联合负压吸引技术在逆行输尿管软镜取石术(RIRS)治疗输尿管上段及肾盂结石的临床效果。方法: 回顾性收集2021年7月至2023年4月在清远市人民医院接受常规截石位联合负压吸引(对照组)及健侧卧奔跑位联合负压吸引(观察组)下施行RIRS的76例输尿管上段及肾盂结石患者的临床资料。统计分析患者临床数据,包括患者术后1天的结石清除率、术后1个月结石清除率、平均手术时间及并发症发生情况。结果: 76例患者均顺利完成手术。对照组45例中,术后1天结石清除率57.8%,术后1个月结石清除率86.7%,平均手术时间为(58.53±15.27)min;术后出现较明显的肉眼血尿3例、发热2例。观察组31例中,术后1天结石清除率90.3%,术后1个月结石清除率93.5%,平均手术时间为(63.39±15.67)min;术后出现较明显的肉眼血尿1例、发热1例。两组患者发热经加强抗感染治疗后均恢复良好。结论: 采用健侧卧奔跑位比常规截石位仅在术后短期(1天)结石清除率上有优势,但在术后1个月结石清除率、平均手术时间、住院时间及术后并发症方面无明显优势。

  • Journal of Jiangsu University(Medicine Edition). 2024, 34(03): 228-233.
    Objective: To explore the regulation and mechanism of microRNA-27a-3p (miR-27a-3p) on ferroptosis of colorectal cancer cell line HT-29 and SW480. Methods: Colorectal cancer cell lines HT-29 and SW480 were treated with 0, 25 and 5 μmol/L Erastin, respectively. Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the mRNA expression of solute carrier family 7 member 11 (SLC7A11) in each group and the optimal concentration of Erastin was screened. HT-29 and SW480 cells were divided into siControl group, siSLC7A11-1 group and siSLC7A11-2 group, and transfected with control siRNA, siSLC7A11-1 and siSLC7A11-2, respectively. The cells were co-cultured with 5 μmol/L Erastin for 0, 24, 48 and 72 h to detect the cell proliferation activity and the level of malondialdehyde in the cells at different time points. HT-29 and SW480 cells were divided into miR-NC group, miR-27a-3p group and anti-miR-27a-3p group, respectively, and transfected with miR-NC, miR-27a-3 mimics and miR-27a-3p inhibitors, respectively. The mRNA expression level of SLC7A11 was detected by qRT-PCR, and the luciferase reporter gene assay was used to detect the luciferase activity of SLC7A11 3′UTR sequence (SLC7A11-WT) and mutant sequence (SLC7A11-Mut). The cells were co-cultured with 5 μmol/L Erastin for 0, 24, 48 and 72 h, and the cell proliferation activity was detected at different time points, and the intracellular malondialdehyde level was detected. Results: The mRNA expression of SLC7A11 in colorectal cancer HT-29 and SW480 cells was significantly increased with the increase of Erastin concentration (P<0.01). In colorectal cancer HT-29 and SW480 cells, compared with the siControl group, the cell proliferation activity in siSLC7A11-1 and siSLC7A11-2 groups was significantly increased at 48 h and 72 h (P<0.05), with a significant decrease in malondialdehyde levels (P<0.05). Compared with the miR-NC group, cell proliferation activity in miR-27a-3p group was significantly enhanced (P<0.05), while malondialdehyde levels and luciferase activity of SLC7A11-WT group were significantly decreased (P<0.05 or P<0.01), and luciferase activity of SLC7A11-Mut group showed no significant changes(P>0.05); and the cell viability of anti-miR-27a-3p group was significantly decreased (P<0.01), the level of malondialdehyde and the luciferase activity of SLC7A11-WT were significantly increased (P<0.01), and the luciferase activity of SLC7A11-Mut was not significantly changed (P>0.05). Conclusion: miR-27a-3p may regulate Erastin-induced ferroptosis in colorectal cancer cells by targeting the 3′UTR of SLC7A11 mRNA.
    Key words]colorectal cancer; ferroptosis; miR-27a-3p; solute carriers 7A11
  • Journal of Jiangsu University(Medicine Edition). 2024, 34(03): 248-253.
    Objective: To observe the clinical efficacy and safety of different types of needle knife combined with compound betamethasone injection under ultrasound guidance in the treatment of trigger finger. Methods: A total of 111 patients diagnosed with trigger finger in the Department of Pain Clinic Affiliated Hospital of Jiangsu University from May 2020 to May 2023 were selected as the study objects. The patients were randomly divided into three groups: group Ⅰ (n=36), group Ⅱ(n=37) and group Ⅲ (n=38). Group Ⅰ: ultrasound guidance + compound betamethasone injection alone group, group Ⅱ: ultrasound guidance + compound betamethasone injection + flat mouth needle knife group, group Ⅲ: ultrasound guidance + compound betamethasone injection + V-type needle knife group, the followup period was 6 months. The visual analogue score (VAS), modified Quinnell grade were recorded before treatment (T1), the next day after treatment (T2), 3 weeks after treatment (T3) and 6 months after treatment (T4),as well as the patient global impression of improvement (PGII) and recurrence rate at 6 months of treatment, operation time, adverse reactions were recorded. Results: ① Comparison of VAS score: At T2 time point, the score of group Ⅱ and group Ⅲ was significantly higher than that of group Ⅰ (P<0.001). The score of group Ⅱ was significantly higher than that of group Ⅲ (P<0.05). At T3 and T4 time points, the scores in group Ⅱ and group Ⅲ were significantly lower than those in group Ⅰ (P<0.05 and P<0.001). There was no significant difference between group Ⅱ and group Ⅲ (P>0.05). ② Comparison of improved Quinnell grading: At T2, T3 and T4 time points, the grade in group Ⅱ and group Ⅲ was significantly lower than that in group Ⅰ (P <0.05 and P<0.001). There was no significant difference in grade between group Ⅱ and group Ⅲ (P>0.05). ③ Comparison of PGII overall improvement scores: The scores of group Ⅱ and group Ⅲ were significantly higher than those of group Ⅰ (P<0.001). There was no significant difference between group Ⅱ and group Ⅲ (P>0.05). ④ Comparison of recurrence rate: It was significantly lower in group Ⅱ and group Ⅲ than that in group Ⅰ (P<0.05), but there was no significant difference between group Ⅱ and group Ⅲ (P>0.05). ⑤ Comparison of operating time: The time of group Ⅱ and group Ⅲ was significantly longer than that of group Ⅰ (P<0.001), and group Ⅱ was significantly longer than group Ⅲ (P<0.05). ⑥ There were no obvious adverse reactions in the three groups. Conclusion: The therapeutic effect of ultrasound-guided needle knife combined with compound betamethasone injection on trigger finger is better than that of compound betamethasone injection alone, and the release effect of V-type needle knife is better than that of flat-mouth needle knife.
    [Key words]trigger finger; ultrasonic guidance; general type needle knife; V-shaped needle knife; acupotomy
  • IANG Xiaofeng, LI Yunzhi, JIN Ming, et al.
    Journal of Jiangsu University(Medicine Edition). 2024, 34(04): 301.
    Objective: To investigate the clinical efficacy of flexible ureteroscopic lithotripsy(FURL) using flexible vacuum suction ureteral access sheath (FVSUAS) in the treatment of upper urinary tract calculi >2 cm, through the combination of crushing and suction technology, without indwelling double J tube before discharge. Methods: Clinical data of 43 patients with >2 cm upper urinary tract stones treated with FVSUAS during FURL from January 2022 to October 2023 were collected and compared with 40 randomly selected patients treated with conventional FURL during the same period. The trial group chose to undergo FURL with FVSUAS and was discharged from the hospital with removal of the ureteral stent tubes 3 days after the operation. In the control group, conventional ureteral access sheath (UAS) was used, and ureteral stent tubes were routinely placed in place postoperatively, and the ureteral stent tubes were removed under second-stage cystoscopy/ureteroscopy at 4 weeks; general data and perioperative indices of patients of the two groups were recorded, and the postoperative stonefree rate, postoperative complications, and patient satisfaction were obtained. Results: The stone-free rate on the 3rd postoperative day in the trial group was 93.02% (40/43), which was significantly higher than that in the control group of 65.00% (26/40), and the difference was statistically significant (P=0.002). When haematuria, pain, and fever between the trial group and the control group on the 3rd postoperative day was compared, the difference was not statistically significant (P>0.05); the proportion of haematuria, pain, and fever in the control group at 4 weeks after the surgery was significantly higher than that of the trial group, the difference was statistically significant (all P<0.05, the satisfaction of the trial group at 4 weeks postoperatively was significantly higher than that of the control group (P<0.01). Conclusion: FURL in combination with FVSUAS for the treatment of >2 cm upper urinary tract stones and tubeless discharge with ureteral stent tubes removed 3 days postoperatively achieves high stonefree rate and significantly reduces the incidence of fever, haematuria and pain complications, and it can be concluded that it is a safe and effective treatment modality.
  • Journal of Jiangsu University(Medicine Edition). 2024, 34(04): 364.
    宫腔粘连是指子宫内膜损伤后导致宫腔或宫颈部分或全部粘连,可产生继发不孕、闭经和周期性下腹痛等症状。宫腔粘连的临床治疗首选宫腔镜下宫腔粘连分离术联合术后辅助治疗,但术后宫腔再次粘连的发生率较高,严重影响女性的生殖潜力。目前,临床上应用于宫腔粘连术后的辅助治疗手段众多,但尚未明确其最佳诊疗方案。仿生物电刺激联合超声针灸是一种新型的物理治疗方式,具有无痛无创、安全、可操作性强等优点,目前已广泛用于治疗盆底功能障碍性疾病。近来,研究发现仿生物电刺激联合超声针灸在治疗宫腔粘连方面有显著疗效,本文对此作一综述。
  • Journal of Jiangsu University(Medicine Edition). 2024, 34(03): 216-221.
    多囊卵巢综合征(polycysticovarysyndrome,PCOS)是常见的生殖内分泌及代谢紊乱性疾病,且PCOS的发生常伴随胰岛素抵抗(insulinresistance,IR),严重危害育龄期女性的身心健康。西医治疗PCOS-IR多以促排卵和增强胰岛素敏感性为主,治疗效果明显,但易反复发作。中医认为肾虚血瘀是PCOSIR发病的基本病机,补肾活血是治疗本病的基本方法,中药、针灸及中药调周法等治疗效果良好。本文对PCOS-IR的中西医治疗进展进行归纳总结,以期为PCOSIR的临床诊疗提供借鉴。
  • Journal of Jiangsu University(Medicine Edition). 2024, 34(06): 514-521.
    Objective: To investigate the effect of bone marrow mesenchymal stem cell-derived exosomes (BMSCs-Exos) on the M1/M2 polarization of alveolar macrophages NR8383 in rats with lipopolysaccharide (LPS)-induced acute lung injury and its potential mechanism. Methods: Bone marrow mesenchymal stem cells were isolated from Sprague-Dawley (SD) rats, and BMSCs-Exos were prepared. NR8383 cells were pretreated with 0.1 or 1 mg/mL BMSCs-Exos for 1 h, followed by induction with 1 μg/mL LPS for 48 h, respectively. ELISA and Western blotting were used to detect the inflammatory factor levels in the cell supernatant and the expression of intracellular polarization marker proteins, respectively. NR8383 cells were cultured alone or co-cultured with BMSCs, treated with 20 μmol/L exosome inhibitor GW4869, and induced with 1 μg/mL LPS for 48 h. Quantitative real-time PCR (qRT-PCR) and Western blotting were used to detect the intracellular miR-212-5p expression and the relative expression of IL-4Rα and p-STAT6 proteins, respectively. The binding site of miR-212-5p and IL-4Rα mRNA were predicted by bioinformatics and verified by luciferase assay. Results: BMSCs-Exos were successfully prepared. ELISA and Western blotting results showed that 1 μg/mL LPS induced for 48 h promoted the secretion of inflammatory factors (TNF-α, IL-1β, and IL-10) and M1 polarization of NR8383 cells. Pretreatment with BMSCs-Exos significantly reduced the contents of LPS-induced inflammatory factors TNF-α and IL-1β, upregulated the content of anti-inflammatory factor IL-10, inhibited M1 polarization and promoted M2 polarization of NR8383 cells, and the effect of 1 mg/mL BMSCs-Exos was significantly stronger than that of 0.1 mg/mL BMSCs-Exos. The cell co-culture experiment results showed that 1 μg/mL LPS induced for 48 h increased miR-212-5p levels and IL-4Rα and p-STAT6 protein expression in NR8383 cells. Co-culture with BMSCs greatly inhibited the effects of LPS on the above indicators, however the effect of BMSCs could be blocked by GW4869. Luciferase assay results indicated that miR-212-5p could bind to the 3′UTR of IL-4Rα mRNA and promote its protein expression. Conclusion: BMSCs-Exos could inhibit M1 polarization and promote M2 polarization of LPS-induced rat alveolar macrophage NR8383 through the miR-212-5p/IL-4Rα/ STAT6 pathway.
    Key words]extracellular vesicles derived from bone marrow mesenchymal cells; acute lung injury; alveolar macrophages; M2 polarization; miR-212-5p