Journal of Jiangsu University(Medicine Edition)

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Journal of Jiangsu University(Medicine Edition)

	2012 Vol.22 Issue.6 Published 2012-11-30

Article

	461	Expression, purification and primary identification of PTD-mLumin-HMGB1 A fusion protein
		GAO Sheng, LIU Yue-fang, TIAN Yi-qing, JI Ying, ZENG Zi-han, YIN Si-yu, SHAO Qi-xiang
		Objective: To express and purify human HMGB1 A box fusion protein, PTD-mLumin-HMGB1 A, which contains a protein transduction domain and a newly isolated far-red fluorescent protein named mLumin, and investigate its ability to across the membrane. Methods: Expression vector of PTD-mLumin-HMGB1 Awas constructed with inserting the HMGB1 A box into pET28a -PTD-mLumin vector. The fusion protein was expressed by E.coli Rossetta (DE3) and purified by Nicolumn. Confocal microscopy was used to confirm the transmembrane ability ofPTD-mLumin-HMGB1 A. Results: The vector of pET28a-PTD-mLumin-HMGB1 A was successfully constructed, and the fusion protein expressed and was purified efficiently.PTD-mLumin-HMGB1 Afusion protein transduced into EL-4 cell efficiently. Conclusion: The PTD-mLumin-HMGB1 Aprotein was successfully constructed, which laid the foundation for further investigation of biological function of human HMGB1 A box.
		2012 Vol. 22 (6): 461-464 [Abstract] ( 2046 ) [HTML 1KB] [ PDF 991KB] ( 2318 )

	465	Optimization on the cultivation method in vitro of cerebral astrocytus from mice
		LIU Xiao-mei1, ZHAO Dan2, PANG Rong-rong2, SHAN Kai2, LI Yan2, WANG Ying-wei2
		Objective: To optimize the cultivation method of cerebral astrocytes from mice in vitro, in which develop an experimental basis for the functional study of astrocytus. Methods: Cerebral cortex was obtained from 0 to 1d C57BL/6 mice and cell suspension was prepared by digestion with trypsin digestion and mechanical dissociation. After differential attachments, primary cells were cultivated for 24 h, so the fresh culture medium instead of the old was added softly. After then, the cell flask was washed shakely before the old culture medium was replaced with the fresh medium every two or three days. After three passages, astrocytus in the culture medium were stained with anti-glial fibrillary acidic protein (GFAP) antibody using immunocytochemical or immunofluorescence staining. Results: After primary cell culture and subculture, the purified astrocytes had the typical shapes with a purity beyond 95%. Conclusion: A reproducible and simple cultivation method in vitro of astrocytus was established, in which high purity astrocytes were obtained.
		2012 Vol. 22 (6): 465-467,471 [Abstract] ( 2042 ) [HTML 1KB] [ PDF 1012KB] ( 3448 )

	468	Isolating, culturing and qualification of mouse pancreatic stellate cells
		FAN Xin1, WANG Ying2, HOU Wen-ji3, LIU Yu3, WANG Xu-qing1, SUN Kang1, ZHANG Jian-xin1
		Objective: To establish a new method to isolate and culture mouse primary pancreatic stellate cells(PSCs). Methods: Based on techniques of isolating and culturing rat PSCs, we acquired purified PSCs from pancreatic tissue fragments via isolation, culture and passage. Oil red O staining, immunofluorescence as well as western blotting were performed to identify pancreatic stellate cells. Cell growth and vitality were validated by cell counting and trypan blue staining. Results: Primary PSCs were positive for oil red O staining; cells grew rapidly and began being activated from the third day. After passage, most of cells were activated. Immunofluorescence staining showed that α-smooth muscle actin (α-SMA) was positive expressed in activated PSCs. Western blotting showed that α-SMA and desmin were expressed in activated PSCs, and that the expression of desmin in fully activated PSCs was obviously higher(P＜0.05).Conclusion: This method can be successfully used to isolate mouse PSCs. The yield, purity and activity of PSCs were higher, and this new method could thus meet the research of pancreatic stellate cells in vitro.
		2012 Vol. 22 (6): 468-471 [Abstract] ( 2330 ) [HTML 1KB] [ PDF 1101KB] ( 2909 )

	472	Effect of gene NDRG1 transfection on the expression of COX-2 and VEGF and proliferation in human cervical cancer cells
		QIAN Hua1, WANG Hua1*, YUAN Dong-lan1, CUI Yong-an2, LIN Mei2, XIAO Wei3, JIAO Xia3， YU Hong3
		Objective: To investigate the effect of N-myc downstream regulated gene 1(NDRG1) on the proliferation in human cervical cancer cell line SiHa, with a focus on the expression of cyclooxygenase-2(COX-2) and vascular endothelial growth factor(VEGF). Methods： pcDNA3.0-NDRG1 and pcDNA3.0 were transfected into SiHa cells through lipofectamine and positive clones were screened by G418. RT-PCR and Western blot were employed to identify mRNA and protein expression of NDRG1 in SiHa cells, respectively. The expression of COX-2 and VEGF in positive clones was detected by Western blot. Cell viability was assessed by MTT method. Results： Positive clone N-12 with NDRG1 overexpression was successfully established. Compared with non-transfected control group, the expression of COX-2 and VEGF was decreased significantly, and the proliferation level of N-12 was also decreased significantly. Conclusion： It is possible that NDRG1 could inhibit the development of cervical cancer by downregulating the expression of COX-2 and VEGF and the proliferation in SiHa cells.
		2012 Vol. 22 (6): 472-475 [Abstract] ( 1495 ) [HTML 1KB] [ PDF 904KB] ( 1790 )

	476	Research on transplantation of brainderived neurotrophic factor transfected bone marrow mesenchymal stem cells in therapy of rats with temporary middle cerebral artery occlusion
		HE Lan-lan1, ZHANG Zun-sheng2, LIU Yong-hai2
		Objective: To detect the expression of brain-derived neurotrophic factor(BDNF)transfected bone marrow mesenchymal stem cells(MSCs) in rat brain, which was subjected to ischemia. Methods： MSCs were transfected with BDNF gene by using lipofect technique and its gene expression was detected by immunocytochemistry. Temporary middle cerebral artery occlusion (MCAO) model was established in adult male SD rats, which were randomly divided into control, PBS transplantation,MSCs transplantation and BDNF-transfected MSCs transplantation groups,with 8 rats in each group. At 3 d after modeling, rats in PBS transplantation, MSCs transplantation and BDNF-transfected MSCs transplantation groups were received with phosphate buffered saline, BrdU(bromodeoxyuridine)-labelled MSCs and BDNFtransfected MSCs injection, respectively, whereas control group with no transplantation. Evaluation of nerve function with neurological injury severity score(NSS) was performed after transplantation. The expression of BDNF, neuron-specific enolase and glial fibrillary acidic protein(GFAP) at 30 d after modeling was determined in all groups. Results： The expression of BDNF in labelled MSCs, at 24 h after transfection, was demonstrated by immunohistochhemistry. There were no significant differences in NSS between all groups at 1 d after transplantation. However, the values of NSS in BDNF-transfected MSCs transplantation group were significantly lower than that in other groups at 7, 14, 21, 30 d after transplantation(P＜0.05). At 30 d after transplantation, the number of positive cells of BDNF in BDNF-transfected MSCs transplantation group was higher than that in MSCs transplantation group, and the number of positive cells of NSE and GFAP in BDNF-tansfected group was higher than that in the other three groups. Conclusion： There was expression of BDNF in cerebral ischemia rat, transplanted with BDNF-transfected MSCs, which reduced brain injury by incerasing the expression of NSE and GFAP secreted by nerve cells and glia cells. Transplantation of BDNF-transfected MSCs might be a novel method for treating cerebral ischemia.
		2012 Vol. 22 (6): 476-482 [Abstract] ( 1274 ) [HTML 1KB] [ PDF 4819KB] ( 2059 )

	483	Inhibitive effects of atorvastatin on homocysteineinduced endoplasmic reticulum stress in human umbilical vein endothelial cells
		JIA Fang1, SUN Jian-hui1, WU Chun-fang2, CHEN Zhen-yue2, LU Guo-ping2
		Objective: To examine the effects of atorvastatin on homocysteine(Hcy)-induced endoplasmic reticulum stress (ERS) activation and cell apoptosis in human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were incubated with various concentrations of Hcy in the presence or absence of atorvastatin. Apoptosis rate was recorded, and expression levels of ERS markers(BiP, CHOP, p-eIF2α and pPERK) were measured by real-time PCR and Western blot analysis. Results： Hcy increased the expression of ERS markers in a dose-dependent manner. Atorvastatin could suppress ERS activation and decrease the apoptosis induced by Hcy in HUVECs. Conclusion: Atorvastatin inhibited Hcy-induced ERS in HUVECs. The protective effect of atorvastatin against Hcyinduced apoptosis was partially mediated by ERS inhibition.
		2012 Vol. 22 (6): 483-486,490 [Abstract] ( 1238 ) [HTML 1KB] [ PDF 1075KB] ( 1887 )

	487	Effect of rapamycin inhibit liver fibrosis in rat with obstructive jaundice
		XU Guangcai, ZHANG Jiafeng, WANG Jian, ZHANG Peijian, JIANG Yongjun, LUO Xiaojiang
		Objective: To study the effect of rapamycin on liver fibrosis in rats with obstructive jaundice, and to explore its mechanism. Methods: The common bile duct was ligated in rats with obstructive jaundice model, 36 SD rats were randomly divided into rapamycin treatment groups (treatment group)(n=18), and the only obstructive jaundice(control group) (n=18). The endotoxin content of liver was monitored each time, while 6 rats of each group were sacrificed on postoperative day (POD)1, 3 and 5,and hepatic tissue samples were harvested to determine TNF-α protein expression levels, with observing the pathological changes of the liver tissue with light microscope.Results: Compared with the control group, the endotoxin level and TNF-α protein expression decreased significantly in treatment group(P＜0.01). The main pathomorphology changes were intrahepatic bile duct proliferation and hepatic fibrosis. In the same time point, pathological changes of the liver tissue was significantly different between the control group and the treatment group. Conclusion: The rapamycin can inhibit liver fibrosis with obstructive jaundice in rats through inhibiting the JAK/STAT signaling pathway.
		2012 Vol. 22 (6): 487-490 [Abstract] ( 1436 ) [HTML 1KB] [ PDF 1003KB] ( 1902 )

	491	Value of detecting the glycosylated hemoglobin in early myocardial injury
		LI Li1, YU Zhengqing1, HU Juan-yu2, CHEN Wei1, ZHAO Guang-chun1, ZHANG Si-jin1, QIAN Lei1
		Objective: To explore the value of detection of glycosylated hemoglobin A1c(HbA1c) for the patients with early myocardial injury and investigate the correlation between HbA1c and highsensitivity cardiac troponin T(hscTnT). Methods： HbA1c was measured in 397 subjects with early myocardial injury, according to level of HbA1c, patients were divided into HbA1c＜5.7% group, 5.7%-6.4% group and ≥6.5% group, at the same time , hscTnT were determined. Results： hscTnT level in HbA1c≥6.5% group was significantly higher than in HbA1c＜5.7% group and 5.7%-6.4% group（P＜0.05）, HbA1c level was significantly positive correlated with hscTnT（r＝0.952 9，P＜0.05）. Conclusion： Higher HbA1c is the independent risk factor of myocardial injury and HbA1c was positive correlated with hscTnT.
		2012 Vol. 22 (6): 491-493 [Abstract] ( 1233 ) [HTML 1KB] [ PDF 871KB] ( 2223 )

	494	Viral etiology of hospitalized children with acute respiratory tract infection
		JU Hui-xiang， ZHOU Zhong-wei， ZHU Rong， TANG Qin-fang
		Objective: To investigate the viral etiology of acute respiratory tract infection（ARI） of hospitalized children in Yancheng of Jiangsu province so as to provide experimental evidence for children with ARI in the area. Methods： The nasopharyngeal aspirate samples from June 2011 to July 2012 were obtained from 1 582 hospitalized children with ARI. Respiratory syncytial virus (RSV), influenza A (IVA), influenza virus B (IVB), parainfluenza virus 1-3 (PIV1-3) and adenovirus were detected by direct immunofluorescence. Results： Four hundred and twenty six case were shown to be infected by respiratory virus in 1582 pediatrics patients and the positive rate was 26.9%. RSV were most frequent（18.3%）, followed by adenovirus（2.9%). The overall detection rate of the virus showed significant differences in different age group and seasonal distribution. The highest detection rate of respiratory virus was among children aged 6 month to 1 year and the incidence was higher in winter and spring than in other seasons. Conclusion： RSV and adenovirus was the predominant pathogens of ARI of hospitalized children in Yancheng and the positive rate was significantly associated with age and seasonal distribution.
		2012 Vol. 22 (6): 494-496 [Abstract] ( 1274 ) [HTML 1KB] [ PDF 796KB] ( 1686 )

	497	Real-time continuous glucose monitoring system in criticallycraniocerebral trauma patients with hyperglycemia
		LV Shao-yan，JI Mu-sen，KONG Xian-ru，CAI Yan，JIN Zhao-chen
		Objective: To access the affects of realtime glucose monitoring system on blood glucose control and mortality in critically craniocerebral trauma patients with hyperglycemia in intensive care unit (ICU). Methods: One hundred and seventeen ICU critical craniocerebral trauma patients with hyperglycemia were studied by RT-CGMS（n=59） and fingertip blood monitoring group (GM group, n=58), and both were worn by RT-CGMS for 72 h(GM group blinded RT-CGMS).Hypoglycemia incidence were observed in the two groups, as well as days in ICU and 30-d mortality. Results: The glucose variability and hypoglycemia incidence in RT-CGMS group were significantly lower than the fingertip blood group，with less days in ICU and 30-d mortality(P< 0.05). Conclusion: RT-CGMS can assist in the smooth control of blood glucose, and reduce the adverse events of hypoglycemia. By optimizing glycemic control, reducing the mortality of hyperglycemia in critically craniocerebral trauma patients.
		2012 Vol. 22 (6): 497-499 [Abstract] ( 1315 ) [HTML 1KB] [ PDF 798KB] ( 2075 )

	500	Expressions of endothelin-1， MMP-2 and CD31 in liver cancer and their significance
		SONG Shu1, XU Chuan-hai1, WANG Zheng-jiang2, SHAO Wei-wei1
		Objective: To examine the expressions of ET-1,MMP-2 and CD31 in liver cancers, and discuss the significance of their expressions. Methods： SP immunohistochemical staining was used to examine the expressions of ET-1, MMP2 and CD31 in 30 normal liver tissues and 30 liver cancers. Results： The different expressions of ET-1, MMP-2 and MVD in normal liver tissues and liver cancers had a statistically significant difference(P<0.01). The expressions of ET-1, MMP-2 and MVD in liver cancers were positively related to clinical stage and differential(P<0.05).But no statistical differences were found between them with age and sex(P＞0.05). MVD in negative expressions of ET-1, MMP-2 were more lower than in positive groups(P<0.05). Conclusions： The expressions of ET-1, MMP-2, MVD were associated with the degree of liver cancer malignancy.The high expressions of ET-1, MMP-2, MVD were correlated with clinical stages and differential degrees in liver cancers.
		2012 Vol. 22 (6): 500-502,506 [Abstract] ( 1463 ) [HTML 1KB] [ PDF 1141KB] ( 2493 )

	503	Association between scores of the motor component of the GCS and outcome after severe traumatic brain injury
		WANG Cun-zu1, XIE Jiang-ning2, CHANG Zhi-ying3, XIE Zheng-xing1, XU Hui-zhong1, DING De-qun4, CHEN Dong-yun5, CHEN Xin-cheng1
		Objective: To analyze association between motor scores and outcome after severe traumatic brain injury, and determine motor scores precision and reliability. Methods: A retrospective analysis was performed of severe traumatic brain injury(TBI) patients; the Glasgow coma scale(GCS) of 140 patients arriving to the hospital was recorded. Prognostic indicators included inhospital mortality during intensive care unit (ICU), days staged in ICU, Glasgow outcome score (GOS) at discharge and six-month after injury in survival patients(>1 month). Results: A total of 140 patients were identified, and 65 (46.4%) were died during ICU. The full GCS, the score of motor response, verbal response and eye opening were four independent variables and had significant correlation with mortality. The score of motor response had significant correlation with GOS at discharge and six-month after injury in long time survival patients(>1 month). Conclusion: The motor response score can accurately predict in-hospital death and outcome in severe TBI patients and be used in pre-hospital assessment.
		2012 Vol. 22 (6): 503-506 [Abstract] ( 1285 ) [HTML 1KB] [ PDF 804KB] ( 2176 )

	507	Effects of ulinastatin on systemic inflammatory response and on S100β protein after thoracotomy in elderly patients with general anesthesia

		[Abstract] Objective: To investigate the effects of ulinastatin on systemic inflammatory response and on S100β protein after thoracotomy in elderly patients with general anesthesia. Methods: One hundred senior patients, undergoing elective general open chest operation were randomly divided into 2 groups: control group (n=50) and ulinastatin group (n=50). In ulinastatin group, ulinastatin(2×104 U/kg) was infused intravenously by pump after the induction of anesthesia; while in control group, equal volume of normal saline was given instead of ulinastatin. Two milliliters of blood samples were taken from vein before operation beginning, 4 h and 24 h after operation termination for determination of TNF-α, IL-6 and S100β protein by means of ELISA. Results: The concentration of TNF-α and IL-6 elevated dramatically after operation in both groups (P<0.05), but TNF-α and IL-6 in ulinastatin group were lower than that at the corresponding time point in control group (P<0.05). The levels of S100β protein were not significantly different before operation and 24 h after operation between two groups, while 4 h after operation the levels of S100β protein in ulinastatin group were lower compared with control group. Conclusion: Ulinastatin could decrease systemic inflammatory response and blood levels of S100β protein after thoracotomy in senior patients, thus decreasing injury to the nervous system.
		2012 Vol. 22 (6): 507-509,517 [Abstract] ( 1467 ) [HTML 1KB] [ PDF 834KB] ( 2320 )

	510	Research on the relationship between body mass index and sleep architecture changes in patients with obstructive sleep apneahypopnea syndrome
		YANG Hua1, XI Feng1, DING Yong-jie2, ZHOU Zhi-cai1
		[Abstract] Objective: To investigate the relationship between body mass index(BMI) and sleep structure in patients with obstructive sleep apneahypopnea syndrome (OSAHS)． Methods： A total of 182 patients were diagnosed by polysomnography(PSG) and divided into 4 groups: snorer, mild, middle and sever．The percentage of sleep stages of non-rapid eye movement and rapid eye movement, apnea-hypopnea index(AHI), BMI and microarousal(MI) were analyzed. Results： There were significant differences in BMI between snorer, mild, middle and sever group, respectively．The percentage of sleep stages(awaking time, S1, S2, S3+S4) also had significant difference in different groups, respectively. The correlation of BMI and AHI, MI and AHI was positive．And there was negative correlation between S3+S4 and AHI． Conclusion： The values of AHI were highly correlated with BMI, high BMI may be involved in the increased severity of OSAHS. Patients with OSAHS had disordered sleep structure, which was significantly associated with the severe degree of the lesion as indicated by AHI．
		2012 Vol. 22 (6): 510-513 [Abstract] ( 1230 ) [HTML 1KB] [ PDF 826KB] ( 1690 )

	514	Shortterm effects of the thoracoscopic and laparoscopic minimally invasive surgery to treat esophageal cancer
		JIANG Guo-jun, ZHOU Jian, GONG Ze-gang, TAN Zhi-gang, TAN Yong-fei
		[Abstract] Objective: To explore the shortterm effects of thoracoscopic and laparoscopic minimally invasive surgery to treat esophageal cancer. Methods： Based on radical surgery of esophageal cancer on patients in our hospital. They were divided into conventional thoracotomy group(n=30) and thoracoscopic combined with laparoscopic esophageal resection group(n=23). To observe the efficacy and complications of both of the methods. Results：Thoracoscopic and laparoscopic resection of esophageal carcinoma took longer operation time, but intraoperative blood loss, postoperative pain, hospitalization time and postoperative respiratory complications were all reduced to compare with the conventional surgery. Both the number of lymph node dissection and postoperative ICU monitoring time had no significant difference.Conclusion： Thoracoscopic and laparoscopic resection of esophageal carcinoma was technically feasible and safe. It had advantages of less trauma, faster recovering and less perioperative complications. It is worthy of clinical promotion.
		2012 Vol. 22 (6): 514-517 [Abstract] ( 1242 ) [HTML 1KB] [ PDF 946KB] ( 2091 )

	518	A comparing study for Conservative treatment and surgical treatment of multiple rib fractures
		YU Shou-qiang1, SUN Bao-lin1, QUAN Yong-hui1, YAN Zheng-yuan1, WEI Chun-sheng1, YANG Xiao-long2
		[Abstract] Objective: Comparison of the advantages and disadvantages between conservative treatment and surgical treatment of multiple rib fractures. Methods： Analysis of the relationship of the two treatment methods with the relevant clinical evaluation parameters by collection of clinical data of 40 cases of conservative treatment and 36 cases of surgical treatment in patients with multiple rib fractures. Results：The time of hospitalization or ICU stay and chest pain relief, respiratory support and the pleural cavity status at discharge were not statistically different (P> 0.05). However, fracture displacement correction rate of rib fractures ,the closed drainage rate of pleural cavity and the total amount of pleural cavity drainage fluid were higher than the conservative group, only the thoracenteses rate in surgery group was lower than conservative group, the difference was statistically significant (P<0.05). Conclusion： Surgical treatment group of multiple rib fractures was superior to conservative group in correcting fracture displacement,but the reduction of chest pain duration in surgical treatment group was not superior to that in conservative group.
		2012 Vol. 22 (6): 518-522 [Abstract] ( 1275 ) [HTML 1KB] [ PDF 865KB] ( 2170 )

	523	Prognosis evaluation of integrative medicine on acute period of ischemic stroke
		DU Xiao, ZHANG Qiu-juan, ZHANG Hong-zhi，SHI Yang
		[Abstract] Objective: To observe combined traditional and western medicine function of treating acute cerebral infarction. Methods: Adopt a multicenter, randomized, controlled study method. A total of 295 cases patients in 5 hospitals with acute cerebral infarction (onset>6 h) were randomly divided into treatment group and control group.Some patients in control group accepted standardizing western medicine treatment and rehabilitation,the others in the experiment group accepted herb, acupuncture, massage,etc. besides western medicine treatment. The two groups accepted 21 days cure for a course of treatment. Count and compare function recovery scale called modified Rankin scores(MRs)in 21 d, 90 d, and 180 d;count and compare independent living ability scale index(BI)in 21 d, 90 d, and 180 d;and observe USA NIH Stroke Scale(NIHSS)score changes in 7 d, 14 d, and 21 d. Results: Therapeutic effectiveness of the treatment group who accepted both Chinese and Western medicine was obvious superior to control group in 180 d(P＜0.05).In the 90th and the 180th day, BI score≥ 85 ratio in treatment group was higher than that in control group(P＜0.05), while the vantage was not obvious in the 21st day. In the 7th, 14th and the 21st day, measured NIHSS score of treatment group was better than that of control group (P＜0.05). Conclusion: The combination of traditional Chinese and Western medicine integrated treatment programs could help ischemic stroke patients in recovery of neurological function, could reduce the disability rate, could improve the ability of daily life of stroke patients.
		2012 Vol. 22 (6): 523-526 [Abstract] ( 1111 ) [HTML 1KB] [ PDF 809KB] ( 1993 )

	527	Expression and significance of nasal IL17A in allergic rhinitis patient after nasal provocation
		HUANG Qiu-sheng, LU Han-qiang, TIAN Shu-yue, RONG Cai-xia
		[Abstract] Objective: To investigate the changes of nasal secretion levels of IL-17A after nasal provocation in allergic rhinitis patients. Methods: The nasal secretion levels of IL-17A was measured by using BDTM Cytometric Bead Array in patients with allergic rhinitis (n=20) compared with those of healthy control subjects (n=20) after using mite nasal provocation. Result： There were significant differences in nasal secretion level of IL-17A between control group and allergic rhinitis patients′ group after nasal provocation 15 minutes, 1 hour, 6 hours (P＜0.05, respectively), however before nasal provocation there were no differences. However there were no differences between patients with allergic rhinitis and control group after nasal provocation 24 hours(P＞0.05). Conclusion: The high expression of nasal secretion levels of IL-17A in the allergic rhinitis patients indicated that IL-17A may be an etiology of allergic rhinitis.
		2012 Vol. 22 (6): 527-529 [Abstract] ( 1210 ) [HTML 1KB] [ PDF 801KB] ( 1892 )

	530	Analysis of Th17 and Treg cells and corresponding transcription factor in peripheral blood from patients with asthma
		RONG Lei, MA Guo-er, BIAN Xiu-juan
		[Abstract] Objective: To study the percentage of CD4+CD25+Foxp3+, CD4+IL-17+ cells and the expression Foxp3, IL-17, RORγt mRNA in peripheral blood from patients with asthma. Methods： The specimens of peripheral blood mononuclear cell(PBMC) were collected from 23 patients of asthma in remission stage, 25 patients of acute paroxysmal asthma and 20 healthy persons(control group). Intracellular flow cytometry detection of the percentage of CD4+CD25+Foxp3+ cells and CD4+IL-17+ cells was established after isolation of untouched human CD4+T cells from PBMC. The expression level of Foxp3, IL-17, RORγt mRNA in CD4+T cells was determined by realtime fluorescence quantitative RT-PCR. Results： Immunomagnetic bead shade selected CD4+T cells reached a purity of more than 90%. The percentage of CD4+CD25+Foxp3+ cells and the expression level of Foxp3 mRNA of the acute paroxysmal group was significantly lower than that of the control group and remission group(P＜0.01), and the comparison of the control group and remission group didn＇t have significant deviation(P＞0.05). The percentage of CD4+IL-17+ cells and the expression level of IL-17、RORγt mRNA of the acute paroxysmal group was significantly higher than those of the control group and remission group (P＜0.01),and the expression level of IL-17、RORγt mRNA in the remission group was also significantly higher than the control group(P＜0.05). Conclusion：The percentage of CD4+CD25+Foxp3+cells and the expression of Foxp3 mRNA in peripheral blood from patients with asthma decreased significantly.The percentage of CD4+IL-17+cells and the expression of IL-17、RORγt mRNA in peripheral blood from patients with athma increased significantly.The imbalance of Treg/Th17 perhaps correlated with the acute episode of asthma.
		2012 Vol. 22 (6): 530-533 [Abstract] ( 1420 ) [HTML 1KB] [ PDF 1030KB] ( 2070 )

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