Journal of Jiangsu University(Medicine Edition)

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Journal of Jiangsu University(Medicine Edition)

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Journal of Jiangsu University(Medicine Edition)

	2012 Vol.22 Issue.2 Published 2012-03-30

Article

	93	Experimental study of TGF-β1-induced human hepatocytes line HL7702 epithelial-mesenchyme transition

		Objective: To investigate whether hepatocytes could undergo epithelial-mesenchyme transition（EMT）on recombinant TGF-β1 stimulation in vitro . Methods: Human hepatocyte line HL7702 were cultured for 2 days with 20% NBS（1640）nutrient medium，and changed for serum-free 1640 medium and TGF-β1 factor induced HL7702 cells for 24，48，72 h. Cells morphology changes were watched before and after induction with inverted microscope. Real-time RT-PCR and western blot were used to analyze the expression of epithelial and mesenchyme cells-associated genes and proteins. Results: Hepatocytes before TGF-β1 induction were irregular sixangle cells. After stimulation cells showed spread and spindle-shaped morphology and cell-cell junction got bigger. Real-time RT-PCR and western blot analysis showed that epithelial marker-associated E-cadherin expression decreased obviously after TGF-β1 induction，while mesenchyme markers N-cadherin、Vimentin、Twist increased significantly. Conclusion: Human hepatocyte line HL7702 happened to EMT by recombinant TGF-β1 stimulation. EMT associated epithelial and mesenchyme genes and proteins expression changed significantly. It could be served as a foundation for further investigation on the mechanism of hepatocytes EMT.
		2012 Vol. 22 (2): 93-96 [Abstract] ( 2673 ) [HTML 1KB] [ PDF 1945KB] ( 3283 )

	97	Effect of gene CTGF transfection on the expression of MMP-2 and TIMP-2 in human breast cancer cells
		XU Bin, CHEN Yin, DAI Gui-Hong, JIANG Xiao-Qin, WANG Wei, ZHAO Guo-Jun, YU Hong
		Objective: To investigate the effect of connective tissue growth factor（CTGF）on the expression of matrix metalloproteinase-2（MMP-2）and tissue inhibitor of metalloproteinase-2（TIMP-2）in human breast cancer line MCF-7. This study was designed to explore the underlying mechanism for the role of CT- GF in the development of breast cancer. Methods: Lipofectin method was used to transfect CTGF vectors into MCF-7; immunofluorescence，RT-PCR and Western blot analysis were used to detect their transfection efficiency. The expressions of MMP-2 and TIMP-2 were determined by Western blot analysis. Results: Im munofluorescence， RT-PCR and Western blot analysis showed a successful transfection of CTGF vectors into MCF-7. MCF-7 transfected with CTGF gene showed an increased expression of MMP-2 protein significantly，compared with control groups. On the other hand，MCF-7 transfected with CTGF gene showed a decreased expression of TIMP-2 protein significantly，compared with control groups. Conclusion: It is possible that CTGF could promote the development of breast cancer by effectively enhancing the expressions of MMP-2 and TIMP-2 in MCF-7 cells.
		2012 Vol. 22 (2): 97-100 [Abstract] ( 1728 ) [HTML 1KB] [ PDF 1233KB] ( 2572 )

	101	Effects of the ethanol extractive of Borago officinalis on neurotransmitter in the brain tissue of mouse model of chronic depression
		GANG Hong-Lin, HE Zhi-Yi, LIU Xiang-Hui, MA Yue
		Objective: The effects of the extractive of Borago officinalis on norepinephrine（NE），dopamine and 5-hydroxytryptamine（5-HT）in mouse model of depression with chronic stress were investigated after establishing of chronic stress mouse models of depression; and to discuss its prevention and cure effects and their possible mechanisms. Methods: Established chronic stress mouse models of depression by using singly housed and long-trem unpredictable mild stress（CUMS）; and to determine the change of brain monoamine neurotransmitters of chronic stress depression model mice by using enzyme-linked immunosorbent assay（ELISA）. Results: The monoamine neurotransmitters（5-HT，NE，dopamine）contents in model mice were significantly less than normal group. The high，medium group of ethanol extractive from Borago officinalis all could increase the contents of 5-HT，NE，dopamine of mice with chronic stress depression;and the low group of ethanol extractive from Borago officinalis approached a raising trend，but no statistical significance. Conclusion: The Borago officinalis can elevate the contents of the 5-HT，NE and dopamine in the brian which were the possible mechanisms of the prevention and cure effect of the Borago officinalis.
		2012 Vol. 22 (2): 101-103 [Abstract] ( 1895 ) [HTML 1KB] [ PDF 768KB] ( 2336 )

	104	Triptolide enhance the sensitivity of doxorubicin via regulating microRNA21 expression in K562/A02 cell line

		Objective: To investigate the reversal effects of triptolide on drug resistance in doxorubicinresistant cells. Methods: Cell viability was measured by MTT assays. The K562/A02 cells were treated by triptolid at non-toxicity concentration. The apoptosis of cells were detected by Annexin V/PI methods.The expression of microRNA21 was measured by fluorescence quantitative polymerase chain reaction. Bcl-2 protein level were measured by western blot. After transfected with microRNA21 antisense oligonucleotide，the sensitivity to doxorubicin，the apoptosis of cells and Bcl-2 protein level were detected in K562/A02 cells. Results: Triptolide at non-toxicity concentration significantly enhanced sensitivity of K562/A02 cells to doxorubicin and promoted doxorubicin-induced apoptosis. Levels of microRNA21and Bcl-2 was significantly decreased after triptolide treatment. Transfection with microRNA21，a significant up-regulation of sensitivity to doxorubicin and a significant down-regulation of Bcl-2 protein was noted in K562/A02 cells. Conclusion: Triptolide significantly sensitizes K562/A02 cell to doxorubicin by inducing apoptosis and these effects of triptolide may be due to its down-regulation of microRNA21.
		2012 Vol. 22 (2): 104-109 [Abstract] ( 4014 ) [HTML 1KB] [ PDF 1435KB] ( 2867 )

	110	Regulation of carbon monoxide-releasing molecule-2 on inflammatory responses in nervous system of septic mice

		Objective: To investigate the regulation effects of exogenous carbon monoxide-releasing molecule-2（CORM-2）on the nervous system in septic mice. Methods: To make standard cecal ligation and puncture（CLP）models，48 C57BL/6 mice were randomly divided into 4 groups: sham group，CLP group，CORM-2 intervention group and Carbachol group. The animals were sacrificed at 24 hours after experimental manipulation. Serum and brain tissue samples were collected，the expression of TNF-α，IL-6，neural expression of anti-inflammatory neurotransmitter acetylcholine（ACh），and the acetylcholinesterase（AChE）activity of brain tissues. To measure the wet-to-dry weight ratio of brain tissues and the pathology changes of brain tissues were also observed by HE dyeing. Results: Compared with the CLP mice，tissues injuries，pro-inflammatory cytokines（TNF-α and IL-6）expressions were markedly down regulated in CORM-2 and Carbachol intervention groups; while the concentration of neurotransmitter ACh were increased，and the ac tivity of AChE were inhibited in CORM-2 and Carbachol intervention mice. Conclusion: Taken together，the data of this study showed that CORM-2 intervention markedly inhibited inflammatory responses in the nervous system，indicating that the CORM-2 may play an important roles in the treatment of sepsis，and will be useful for the clinical treatment of critically ill patients in the future.
		2012 Vol. 22 (2): 110-113 [Abstract] ( 1755 ) [HTML 1KB] [ PDF 5815KB] ( 2614 )

	114	Influence of RNase G on the regulation of non-coding RNA T3956 in Salmonella enterica serovar Typhi

		Objective: To investigate the influence of RNase G on the regulation of non-coding RNA（ncRNA）T3956 in Salmonella enterica serovar Typhi（S . Typhi）. Methods: The rng deleted mutant of S . Typhi was prepared by the homologous recombination mediated by suicide plasmid; the rng complementary strain was generated by transferring the recombinant plasmid pBAD rng into the rng deleted mutant; qRT-PCR was performed to analyze the level of non-coding RNA T3956 in the wild strain，the rng mutant strain，the complementary strain and the control strain at different growth phases. Results: The rng deleted mutant of S . Typhi，the rng complementary strain and the control strain were constructed successfully. The results of qRT-PCR revealed that the cellular level of T3956 was increased in the rng mutant comparing to the wild type strain，especially at mid-log phase and stationary phase，and the level of T3956 was restored in the rng complementary strain. Conclusion: RNase G was involved in the regulation of the ncRNA T3956 levels in S . Typhi，and played a more important role in the regulation at mid-log and stationary phase.
		2012 Vol. 22 (2): 114-119 [Abstract] ( 2428 ) [HTML 1KB] [ PDF 998KB] ( 2769 )

	120	Construction and indentification of recombinant adenovirus vector containing mouse IFN-λ2 gene

		Objective: To construct recombinant adenovirus vector containing mouse IFN-λ2 gene. Methods: Full-length of mIFN-λ2 cDNA was obtained by using RT-PCR from cells of mouse spleen stimulated by vesicular stomatovirus and then subcloned to the vector of pShuttle- CMV. Generation of recombinants by cotransforming the PmeⅠ-cut shuttle plasmids with pAdEasy-1 backbone vectors in BJ5183，virus was amplified in 293 cells. RT-PCR was used to identify mIFN-λ2 gene had transformed into recombinant adenovirus，and the method of TCID₅₀ was used to measure the virus titer. The mouse lung adenocarcinoma cells were infected by the recombinant adenovirus，the expression of mIFN-λ2 was detected by Western bolt，and the inhibitory effect of mIFN-λ2 to mouse lung adenocarcinoma cells was observed by inverted microscope. Results: The sequence of mIFN-λ2 gene was identical with that reported in GenBank.The recombinant adenovirus vector was successfully constructed and verified by restriction endonucleases digestion. Recombinant adenovirus was successfully produced and amplified in 293A cell line. The constructed recombinant adenovirus was verified containing mouse IFN-λ2 gene，and its virus titer was 2×10¹⁰ pfu/ml. Western bolt indicated mIFN-λ2 protein was expressed in the supernatant of mouse lung adenocarcinoma cells infected by the recombinant adenovirus.The inhibitory effect of mIFN-λ2 to mouse lung adenocarcinoma cells was identified by inverted microscope. Conclusion: The adenovirus vector of mIFN-λ2 was successfully constructed.The mIFN-λ2 protein was expressed on mouse lung adenocarcinoma cells infected by the recombinant adenovirus and the growth of mouse lung adenocarcinoma cells was inhibited. This result lays not only a solid foundation for a further study of the impact of the IFN-λ on tumor but also for gene therapy.
		2012 Vol. 22 (2): 120-124 [Abstract] ( 1813 ) [HTML 1KB] [ PDF 3100KB] ( 2204 )

	125	Expression of VEGF and VEGFR in the pulp of different developmental state of permanent teeth

		Objective: To investigate the histological and molecule level expression and distribution of VEGF，Flt-1，Flk-1 in the pulp of young and mature permanent teeth and explore the role of VEGF，Flt-1，Flk-1 in the permanent root development. Methods: Young and mature permanent teeth were collected for orthodontic reasons，each group included 15 teeth. The expression and localization of VEGF，Flt-1，Flk-1 were studied with immunohistochemistry. VEGF，Flt-1，Flk-1 mRNA levels of young and mature permanent teeth dental pulp were assayed by RT-PCR. Results: Immunohistochemical staining detected the expression of VEGF and Flk-1，they were more positive in young permanent than the mature（P <0.05）; while Flt-1 was more positive in the mature; RT-PCR demonstrated VEGF，Flt-1，Flk-1 mRNA expression in young and mature permanent teeth pulp tissue with 145 bp，169 bp and 162 bp transcript respectively，and relative content of VEGF，Flt-1，Flk-1 in young permanent teeth were 1.7，0.6 and 1.5 times in mature ones. Conclusion: The expression of VEGF，Flt-1，Flk-1 in the pulp of young and mature permanent teeth showed different characteristics，which suggests that the reparative dentine formation of permanent teeth can be induced by regulating the upper genes at different development stages.
		2012 Vol. 22 (2): 125-129 [Abstract] ( 1805 ) [HTML 1KB] [ PDF 15733KB] ( 1331 )

	130	Mechanism of tetrandrine in inhibiting proliferation of drug resistant hepatocellular carcinoma cells

		Objective: Drug resistant hepatocellular carcinoma cell line was treated with tetrandrine （TET） to investigate the possible mechanism of TET alone or in combination with anti-cancer drug in inhibiting proliferation of drug resistant hepatocellular carcinoma cell line. Methods: Drug resistant hepatocellular carcinoma cell BEL-7402/DOX was treated with TET alone or in combination with doxorubicin（DOX），the inhibition rate of drug resistant hepatocellular carcinoma cells was measured with MTT. The intracellular DOX concentration was calculated with the D value measured with fluorescent spectrometer. The intracellular calcium concentration was measured with Fura-2/AM. Results: TET inhibited proliferation of BEL- 7402/DOX cells and enhanced the inhibitory effect of DOX in BEL-7402/DOX cells in a dose-dependent manner. Different concentrations of TET in combination with DOX significantly increased the intracellular DOX concentration in BEL-7402/DOX cells（P <0.05～0.01）. TET with the concentration of 5.0 mg/L and 10.0 mg/L both significantly reduced the intracellular calcium concentration in BEL-7402/DOX cells （P <0.05，P <0.01）. Conclusion: The mechanism of TET alone or in combination with anti-cancer drug in inhibiting proliferation of drug resistant hepatocellular carcinoma cell line may be related to reduction of intracellular calcium concentration， inhibition of signal transmission and increase in intracellular DOX concentration.
		2012 Vol. 22 (2): 130-132 [Abstract] ( 2299 ) [HTML 1KB] [ PDF 795KB] ( 2649 )

	133	Mitral valve repairment through a minimal right vertical infra-axillary thoracotomy versus standard median sternotomy

		Objective: To compare the right vertical infra-axillary thoracotomy for mitral valve repairment with standard median sternotomy. Methods: A total of 93 patients，divided into a right vertical infra-axillary thoracotomy group and a standard median sternotomy group，who underwent mitral valvuloplasty from January 2002 to November 2010 were analyzed retrospectively. Results: Hospital mortality was 1 of 36 （2.8%）patients in the right vertical infra-axillary thoracotomy group and none in the standard median sternotomy group. There was no late death in either group. The time to establish cardiopulmonary bypass was longer in the right vertical infra-axillary thoracotomy group（P <0.05）. The right vertical infra-axillary thoracotomy group had less chest drainage during the postoperative 24 hours than the standard median sternotomy group and required less blood transfusion（P <0.05）. Postoperative mechanical ventilation time was also less in the right vertical infra-axillary thoracotomy group（P <0.05）. There were no statistical differences in aortic cross-clamp time，cardiopulmonary bypass time，and total operation time between the two groups. Conclusion: The right vertical infra-axillary thoracotomy can be performed with favorable cosmetic and clinical results. It provides a good alternative to standard median sternotomy for mitral valve repairment.
		2012 Vol. 22 (2): 133-136 [Abstract] ( 1726 ) [HTML 1KB] [ PDF 766KB] ( 2225 )

	137	Effect of morphine on the expression of survivin and livin in human breast cancer cells

		Objective: To investigate the effect of morphine on proliferation and apoptosis in human breast cancer cell line MCF-7，with a focus on the expression of survivin and livin. MCF-7 cells were seeded in 6-well plates（1×10³ /ml） and divided into 3 groups: groupⅠnormal control（group C），groupⅡ was exposed to 0.1μmol/L morphine（group M1），groupⅢ was exposed to 1.0μmol/L morphine（group M2）. The expression of survivin and livin in different group of cells was detected by RT-PCR and Western blot at 24 hours of incubation with morphine. The apoptosis of cells was determined by flow cytometry at 24 hours of incubation with morphine. The proliferation of cells was determined by Thiazolyl blue tetrazolium bromide（MTT）method at 24，48，and 72 hours of incubation with morphine respectively. Results: Compared with group C，the expression of survivin and livin，and the proliferation level of group M1 and M2 was decreased significantly（P< 0.01），however，the apoptosis rate of cells was increased significantly（P< 0.01）. Conclusion: Morphine could inhibit the proliferation of the cells and accelerate the cell apoptosis through down-regulating the expression of survivin and livin genes in human breast cancer cell line MCF-7.
		2012 Vol. 22 (2): 137-140 [Abstract] ( 1737 ) [HTML 1KB] [ PDF 1148KB] ( 2026 )

	141	Observation of therapeutic effects of the early combination of sertraline and flupentixol melitracen in treatment of post-stroke depression

		Objective: To explore clinical effectiveness of early combination sertraline and flupentixol melitracen in the treatment of post-stroke depression. Methods: Ninety patients were randomly divided into two groups，combined treatment group（treated with combination of sertrline and flupentixol melitracen for 2 weeks and then treated with sertraline for 2 weeks）and control group（with sertraline）for four weeks. All the patients were scored by Hamilton Depression Scale（HAMD）、National Institutes of Health Stroke Scale （NIHSS）and Barthel index（BI）scores before treatment and at the end of 1st，2nd，and 4th week after treatment. Results: Compared with pretreatment in both groups，the HAMD、NIHSS scores after treatment were decreased with treatment time（P <0.05），the BI index with the treatment time gradually improved and there were more significant difference（P <0.05）. There were more significant difference of HAMD、NIHSS and BI scores at the end of 1st and 2nd week between 2 groups. There were little difference at the end of 4th week（P >0.05）. The incidence of adverse effects in combined treatment group were significantly less than the control group（P <0.05）. Conclusion: The early combination of sertraline and flupentixol melitracen in the treatment of post-stroke depression works fast and has better clinical effectiveness，less adverse effects， better compliance，and is conductive to the rehabilitation of neurological function and activities of daily living recovery.
		2012 Vol. 22 (2): 141-143 [Abstract] ( 2424 ) [HTML 1KB] [ PDF 759KB] ( 2537 )

	144	Anterior debridement，bone graft and posterior internal fixation in the treatment of thoraciclumbar spinal tuberculosis

		Objective: To evaluate the clinical efficacy of anterior debridement，bone graft and posterior internal fixation in the treatment of thoracic lumbar spinal tuberculosis. Methods: Twenty patients，including 10 males and 10 females; after routine 2 to 4 weeks anti-tuberculosis chemotherapy，the patients were treated with anterior debridement，with iliac or rib bone graft and posterior internal fixation，continued anti-tuberculosis chemotherapy for one year after operation.Calculated the preoperation and postoperation kyphosis cobb angle（x± s）of the patients，analyze d the difference in statistics. Analyzed the preoperation and postoperation spinal cord and neurologic function in ASIA classification. Results: The postoperation follow up time was 10 months to 3 years，average 15 months.Recurrence of the disease in 1 patient.The average fusion time was 6（3-10）months. The complication was chronic sinus in 1 patient，fully recovered after sinus debridement.Analyzed the preoperation and postoperation kyphosis cobb angle（x± s） of the patients，the difference was significant in statistics（P <0.05）.All cases both acquired improvement in neurologic function. Conclusion: One stage anterior debridement，bone graft and posterior internal fixation in the treatment of thoracic lumbar spinal tuberculosis can get fully clearance completely，correction of the kyphosis deformity，improvement of the neurologic function.
		2012 Vol. 22 (2): 144-146 [Abstract] ( 1531 ) [HTML 1KB] [ PDF 798KB] ( 2042 )

	147	Analysis of fatty acids from Hibiscus sabdariffa L. calyx by GC-MS

		Objective: To analyze the fatty acids from Hibiscus sabdariffa L. calyx by gas chromatography-mass spectrometry（GC-MS）. Methods: To extract oil from Hibiscus sabdariffa L. calyx by soxhlet extraction method. After methyl esterification，the oil was analyzed by GC-MS. Results: The oil from Hibiscus sabdariffa L. calyx was composed of seventeen fatty acids. The main fatty acids of the oil were linoleic acid （45.03%），oleic acid（23.62%），palmitic acid（22.91%），stearic acid（3.41%），（10 E）-10-nonadecenoic acid（1.91%），palmitoleic acid（0.84%），eicosanoic acid（0.53%）and myristic acid （0.34%），respectively. The content of unsaturated fatty acids was up to 71.40%. Conclusion: Unsaturated fatty acids，rich in Hibiscus sabdariffa L. oil，are of high nutrition for human health.
		2012 Vol. 22 (2): 147-150 [Abstract] ( 2025 ) [HTML 1KB] [ PDF 931KB] ( 2499 )

	151	Preparation and characterization of mesoporous silica SBA-15-supported molecularly caffeine-imprinted polymers by surface molecularly imprinting technique

		Objective: The adsorption behavior of the caffeine molecularly imprinted polymer for caffeine was investigated. Moreover，the imprinted polymer was applied for selective extraction of caffeine from tea. Methods: A novel molecularly imprinted polymer was prepared with caffeine as template and modified ordered mesoporous silica SBA-15 as carrier based on surface molecularly imprinting technique. The prepared polymer was characterized by the scanning electron microscopy. The adsorption behavior of the adsorbent for caffeine was investigated using batch experiments. In addition，these imprinted polymers were applied for selective extraction of caffeine from tea. Results: The optimum acidity was around pH 7.0 and the imprinted polymer exhibited fast kinetics and high adsorption capacity for caffeine. Caffeine adsorption process onto MIPs followed the Freundlich adsorption isotherm and the Pseudo-second-order kinetic model. Furthermore，these imprinted polymers could extract caffeine from tea effectively. Conclusion: MIT looks forward to be used as the materials for extraction of caffeine from tea.
		2012 Vol. 22 (2): 151-154 [Abstract] ( 2322 ) [HTML 1KB] [ PDF 1638KB] ( 2794 )

	155	Study on orthogonal test and medium enlarge test of extraction process of Shenyanxiaobai granules

		Objective: To find out the optimum water extraction process for the preparation of Shenyanxiaobai granules. Methods: During the study orthogonal test was used to find out the optimum extraction process of Shenyanxiaobai granules，with weights of total dissolved solids and Astragaloside IV content taken as targets，then medium enlarge test was studied. Results: The laboratory method determined the extraction process as follows: Eight times of water and extracting 2 h for 3 times，and the inventory rating was 132 g （1/10 recipe quantity）. Meanwhile，the optimum extraction process got by medium enlarge test was 8 times of water and extracting for 3 times. The first and second time extracted 1.5 h，and the last time extracted 1h. The inventory rating was 15.84. Conclusion: There was difference between the extraction processes determined by laboratory study and medium enlarge test.
		2012 Vol. 22 (2): 155-158 [Abstract] ( 1258 ) [HTML 1KB] [ PDF 762KB] ( 1986 )

	159	GC/MS analysis of water-soluble essential oil from root of Rhodiola sachalinensis A.Bor.

		Objective: To analysis the chemical constituents of the water-soluble essential oil from Rhodiola sachalinensis A.Bor. grown in the Changbai Mountain of Jilin. Methods: The water-soluble essential oil was extracted by steam distillation and analyzed by GC-MS. Results: A total of twenty-five components were separated and 18 constituents were identified which occupied 90.17% of essential oils. The major constituents were Myrtanol（28.04%），cis-Linaloloxide（16.90%），1-Octanol（10.05%），etc. Conclusion: The results provided theoretical foundation for product development.
		2012 Vol. 22 (2): 159-161 [Abstract] ( 2017 ) [HTML 1KB] [ PDF 804KB] ( 2475 )

	162	Optimized preparation of etofibrate sustained release capsule by central composite design and response surface method

		Objective: Prepare Etofibrate sustained release capsule;To optimize the formulation of Etofibrate sustained release capsule by Central Composite Design-Response Surface Method Ology（CCD-RSM）. Methods: Etofibrate pellets were prepared by centrifugal granulation equipment，then they were coated by solution of PEG4000 and Etofibrate dissolute in Acetone. Aqueous dispersion of Acrylic Resins（Eudragit RL30D）was used for the sustained release layer coating. In the formulation design using CCD-RSM，independent variables were the mass ratio of solubilize layer，the mass ratio of PEG4000 in the solubilize layer，and the weight gain percentage of sustained release layer. The percentage of in vitro cumulative releases at 4 h，8 h and 24 h were dependent variables. Multilinear and quadratic models were used to estimate the relationship between the dependent and the independent variables，and to delineate RSM and overlay contour plots in order to select the optimal formulation. Results: The optimized formulation parameter was: weight ratio of solubilize layer was between 0.7-1.2;the mass ratio of PEG4000 in the solubilize layer was between 3.5%-12.8%;the weight gain percentage of sustained release layer was between 8.0%-13.5 %. Quadratic model showed better prediction capability than multilinear model. The quantitative relationships between three factors and three evaluation indexes were characterized. Moreover，in vitro release test of the selected optimal formulation indicated thigh approximation between the observed and estimated values. Conclusion: The CCD-RSM can be applied to optimize the formulation of Etofibrate sustained release capsule and the established model is of satisfactory predictive value.
		2012 Vol. 22 (2): 162-166 [Abstract] ( 1628 ) [HTML 1KB] [ PDF 2993KB] ( 2198 )

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