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Influence of RNase G on the regulation of non-coding RNA T3956 in Salmonella enterica serovar Typhi |
(School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang Jiangsu 212013, China) |
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Abstract Objective: To investigate the influence of RNase G on the regulation of non-coding RNA(ncRNA)T3956 in Salmonella enterica serovar Typhi(S . Typhi). Methods: The rng deleted mutant of S . Typhi was prepared by the homologous recombination mediated by suicide plasmid; the rng complementary strain was generated by transferring the recombinant plasmid pBAD rng into the rng deleted mutant; qRT-PCR was performed to analyze the level of non-coding RNA T3956 in the wild strain,the rng mutant strain,the complementary strain and the control strain at different growth phases. Results: The rng deleted mutant of S . Typhi,the rng complementary strain and the control strain were constructed successfully. The results of qRT-PCR revealed that the cellular level of T3956 was increased in the rng mutant comparing to the wild type strain,especially at mid-log phase and stationary phase,and the level of T3956 was restored in the rng complementary strain. Conclusion: RNase G was involved in the regulation of the ncRNA T3956 levels in S . Typhi,and played a more important role in the regulation at mid-log and stationary phase.
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. [J]. Journal of Jiangsu University(Medicine Edition), 2019, 29(04): 360-365. |
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