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| Mechanism of tetrandrine in inhibiting proliferation of drug resistant hepatocellular carcinoma cells |
| （1.School of Clinical Medicine， Jiangsu University， Zhenjiang Jiangsu 212013; 2.Department of Gastroenterology， Kunshan Hospital Affiliated to Jiangsu University， Kunshan Jiangsu 215300， China） |
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Abstract Objective: Drug resistant hepatocellular carcinoma cell line was treated with tetrandrine （TET） to investigate the possible mechanism of TET alone or in combination with anti-cancer drug in inhibiting proliferation of drug resistant hepatocellular carcinoma cell line. Methods: Drug resistant hepatocellular carcinoma cell BEL-7402/DOX was treated with TET alone or in combination with doxorubicin（DOX），the inhibition rate of drug resistant hepatocellular carcinoma cells was measured with MTT. The intracellular DOX concentration was calculated with the D value measured with fluorescent spectrometer. The intracellular calcium concentration was measured with Fura-2/AM. Results: TET inhibited proliferation of BEL- 7402/DOX cells and enhanced the inhibitory effect of DOX in BEL-7402/DOX cells in a dose-dependent manner. Different concentrations of TET in combination with DOX significantly increased the intracellular DOX concentration in BEL-7402/DOX cells（P <0.05～0.01）. TET with the concentration of 5.0 mg/L and 10.0 mg/L both significantly reduced the intracellular calcium concentration in BEL-7402/DOX cells （P <0.05，P <0.01）. Conclusion: The mechanism of TET alone or in combination with anti-cancer drug in inhibiting proliferation of drug resistant hepatocellular carcinoma cell line may be related to reduction of intracellular calcium concentration， inhibition of signal transmission and increase in intracellular DOX concentration.
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2012, Vol. 22 
