Abstract：Objective: To investigate the effect of aerobic exercise on cell apoptosis and expressions of Bcl-2 and Bax protein in skeletal muscle from aging model rats. Methods: The aging model rats were randomly divided into control group and two exercise groups which include 6 times/wk exercise group and 3 times／wk exercise group. Exercise groups were trained by swimming for 12 weeks, 90 min each time. All rats were killed after 48 hours of the last training session. Cell apoptosis of skeletal muscle was detected by using TUNEL staining. The expressions of Bcl-2 and Bax in skeletal muscle were quantified by western blotting. Results: Compared with control group, the apoptotic index(AI) of skeletal muscle and expression of Bax protein in exercise groups were obviously decreased (both P<0.01), while the expression of Bcl-2 protein and the ratio of Bcl-2/Bax were markedly increased (both P<0.01). Compared with 6 times／wk group, the AI of skeletal muscle and expression of Bax protein in 3 times/wk group were significantly decreased(P<0.05), whereas the expression of Bcl2 protein and the ratio of Bcl-2/Bax were greatly increased(P<0.05). Conclusion: The ratio of Bcl-2/Bax could be increased by aerobic exercise. And the excessive apoptosis of skeletal muscle of aged rats could be reduced by aerobic exercise.The effect of everyotherday aerobic exercise was better than everyday aerobic exercise. It was beneficial to inhibit cell apoptosis of skeletal muscle.
刘宁宁1, 李明学2, 金雯1. 有氧运动对衰老大鼠骨骼肌细胞凋亡及Bcl-2和Bax表达的影响[J]. 江苏大学学报:医学版, 2013, 23(3): 229-.
LIU Ning-Ning-1, LI Ming-Xue-2, JIN Wen-1. Influence of aerobic exercise on cell apoptosis and expressions of Bcl-2 and Bax protein in skeletal muscle from aging model rats. Journal of Jiangsu University(Medicine Edition), 2013, 23(3): 229-.
2013, Vol. 23 
