Abstract：Objective: To study the clinical significance of mRNA expressions of epithelial cell adhesion molecule(EpCAM) and mucin1(MUC1) in peripheral blood from patients with non-small cell lung cancer(NSCLC). Methods: The mRNA expressions of EpCAM and MUC1 in peripheral blood samples from 45 patients with NSCLC and 21 patients with benign pulmonary disease were detected by quantitive real-time polymerase chain reaction (qRT-PCR). Correlations between the two mRNA expressions and clinicopathologic characteristics of NSCLC patients were analysed. Results: The levels of mRNA expressions of EpCAM and MUC1 in patients with NSCLC were significantly higher than those in patients with benign pulmonary disease(4.42±0.56 vs 2.48±0.26, P=0.025 for EpCAM; 2.30±0.32 vs 1.61±0.06, P=0.018 for MUC1). There were significant differences between NSCLC patients and patients with benign pulmonary disease with regard to the positive rates of mRNA expressions of EpCAM and MUC1(both P＜0.01). The two mRNA expressions were not significantly correlated with age, gender and histopathologic type, but correlated with TNM staging of tumor; and the positive rates of mRNA expressions of EpCAM and MUC1 in peripheral blood from patients with stage Ⅰ+Ⅱ NSCLC were higher than those of patients with stage Ⅲ+Ⅳ NSCLC(P=0.021 and P=0.005, respectively). Conclusion: Detection of EpCAM mRNA and MUC1 mRNA in peripheral blood could be favourable for the clinical diagnosis of NSCLC, especially for detection of blood micrometastasis.
朱文芳，李坚，唐兴萍. 非小细胞肺癌患者外周血上皮细胞黏附分子和黏蛋白的诊断价值[J]. 江苏大学学报:医学版, 2013, 23(2): 144-148.
ZHU Wen-fang, LI Jian, TANG Xing-ping. Diagnostic value of epithelial cell adhesion molecule and mucin1 in peripheral blood from patients with non-small cell lung cancer. Journal of Jiangsu University(Medicine Edition), 2013, 23(2): 144-148.
［1］Glinsky VV, Glinsky GV, Glinskii OV, et al. Intravascular metastatic cancer cell homotypic aggregation at the sites of primary attachment to the endothelium［J］. Cancer Res, 2003, 63(13):3805-3811.［2］Matsunaga H, Hangai N, Aso Y，et al. Application of differential display to identify genes for lung cancer detection in peripheral blood［J］．Int J Caner, 2008，100(5):592-599．［3］Osaki T，Oyama T，Gu CD, et al．Prognostic impact of micrometastatic tumor cells in the lymph nodes and bone marrow of patients with completely resected stage Ⅰ nonsmallcell lung cancer［J］. J Clin Oncol, 2010, 20(13):2930-2936.［4］Khan MS, Tsigani T, Rashid M, et al. Circulating tumor cells and EpCAM expression in neuroendocrine tumors［J］. Clin Cancer Res, 2011, 17(2):337-345.［5］Yanamoto S, Kawasaki G, Yoshitomi I, et al. Clinic pathologic significance of EpCAM expression in squamous cell carcinoma of the tongue and its possibility as a potential target for tongue cancer gene therapy［J］. Oral Oncol, 2007, 43(9):869-877. ［6］Gastl G, Spizzo G, Obrist P, et al. EpCAM over expression in breast cancer as a predictor of survival［J］. Lancet, 2010, 356(9246):1981-1982.［7］Karanikiotis C, Skiadsa I, Karina M, et al. A novel chromatographic method for Ep-AM mRNA detection in peripheral blood and bone marrow of patients with metastatic colorectal cancer［J］. Anticancer Res, 2005, 25(1A):319-323.［8］Thirkill L, Gao T, Stout M, et al. MUC1 is involved in trophoblast transendothelial migration ［J］.Biochim Biophys Acta, 2007, 1773(6):1007-1014.［9］蔡祖勋，梁庆正，张学完，等.非小细胞肺癌组织中p53和MUC1的表达及其意义［J］.肿瘤防治杂志，2004, 11(7):694-696.［10］郑顺利，杨庆生，马小红.非小细胞肺癌患者纵隔淋巴结和外周血中MUC1基因的检测及意义［J］．第三军医大学学报， 2006, 28(24):2472-2474.［11］Ono T, Minamiya Y, Ito M, et al. Sentinel node mapping and micrometastasis in patients with clinical stage IA non-mall cell lung cancer ［J］. Interact Cardiovasc Thorac Surg, 2009, 9(4):659-661.
2013, Vol. 23 
