Abstract:Objective: To study the clinical significance of mRNA expressions of epithelial cell adhesion molecule(EpCAM) and mucin1(MUC1) in peripheral blood from patients with non-small cell lung cancer(NSCLC). Methods: The mRNA expressions of EpCAM and MUC1 in peripheral blood samples from 45 patients with NSCLC and 21 patients with benign pulmonary disease were detected by quantitive real-time polymerase chain reaction (qRT-PCR). Correlations between the two mRNA expressions and clinicopathologic characteristics of NSCLC patients were analysed. Results: The levels of mRNA expressions of EpCAM and MUC1 in patients with NSCLC were significantly higher than those in patients with benign pulmonary disease(4.42±0.56 vs 2.48±0.26, P=0.025 for EpCAM; 2.30±0.32 vs 1.61±0.06, P=0.018 for MUC1). There were significant differences between NSCLC patients and patients with benign pulmonary disease with regard to the positive rates of mRNA expressions of EpCAM and MUC1(both P<0.01). The two mRNA expressions were not significantly correlated with age, gender and histopathologic type, but correlated with TNM staging of tumor; and the positive rates of mRNA expressions of EpCAM and MUC1 in peripheral blood from patients with stage Ⅰ+Ⅱ NSCLC were higher than those of patients with stage Ⅲ+Ⅳ NSCLC(P=0.021 and P=0.005, respectively). Conclusion: Detection of EpCAM mRNA and MUC1 mRNA in peripheral blood could be favourable for the clinical diagnosis of NSCLC, especially for detection of blood micrometastasis.
朱文芳,李坚,唐兴萍. 非小细胞肺癌患者外周血上皮细胞黏附分子和黏蛋白的诊断价值[J]. 江苏大学学报:医学版, 2013, 23(2): 144-148.
ZHU Wen-fang, LI Jian, TANG Xing-ping. Diagnostic value of epithelial cell adhesion molecule and mucin1 in peripheral blood from patients with non-small cell lung cancer. Journal of Jiangsu University(Medicine Edition), 2013, 23(2): 144-148.
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