Abstract：Objective: To detect recombinant human erythropoietin(rh-EPO) on the biological behavior of tumor cells such as proliferation, apoptosis and metastasis; and to explore the role of EPO and EPO receptor(EPO-R) in the development of breast cancer.Methods: The expression levels of EPO and EPOR in MCF-7 and MDAMB-231 were detected by RT-PCR,immunocytochemistry and Western blotting. The proliferation abilities of MCF-7 and MDA-MB-231 cells interfered by rh-EPO in different concentration were detected by MTT assay.The Apoptosis of MCF-7 and MDA-MB-231 cells were evaluated by the terminal deoxynucleotidyl transferase mediated dutp-Biotin nick endlabeling assay（TUNEL）.The migration and invasion abilities of MCF-7 and MDA-MB-231 cells were investigated by transwell technique. Results: The results of RT-PCR and Western blotting showed that EPO and EPO-R were expressed in MCF-7 and MDA-MB-231.The MTT assay and invasion assay revealed that rh-EPO could enhance the capacity of proliferation and the ability of migration and Matrigel-invasion in vitro both in MCF-7 and MDA-MB-231 cell lines in a time and dose-dependent manner. In TUNEL, the results showed that rh-EPO failed to prevent apoptosis. Conclusion: rh-EPO could enhance the capacity of proliferation and the ability of migration and Matrigel-invasion in vitro both in MCF-7 and MDA-MB-231 cell lines, and the effect on MCF-7 cells was better than that on MDA-MB-231 cells, but could not induce cell apoptosis.
收稿日期: 2013-01-02
基金资助:
福建省自然科学基金资助项目（2009J01155）
作者简介: 晋雯（1969—），女，福建福州人，副教授，硕士生导师，主要从事肿瘤病理学研究。
引用本文:
晋雯，张小容，孔令英. 促红细胞生成素在人乳腺癌细胞株中的表达及作用[J]. 江苏大学学报:医学版, 2013, 23(2): 124-129.
JIN Wen，ZHANG Xiao-rong, KONG Ling-ying.
Expression and function of erythropoietin in human breast cancer cell lines. Journal of Jiangsu University(Medicine Edition), 2013, 23(2): 124-129.
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