转化生长因子-β1在多柔比星致大鼠心肌细胞凋亡中的促进作用

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摘要目的：探讨转化生长因子-β1(TGF-β1)在多柔比星诱导大鼠心肌细胞凋亡中的作用。方法：取出生1～2 d的SD大鼠心肌细胞进行体外分离及纯化，用α-横纹肌肌动蛋白（α-SA）免疫化学染色法鉴定细胞纯度。将培养第3天的心肌细胞随机分为对照组和不同浓度（0.5，1，2 μmol/L）多柔比星处理组，同时对各浓度组的不同时间点（4，12，24，48，72 h）进行检测。用MTT法检测心肌细胞存活率，流式细胞术Annexin Ⅴ/PI双染法检测心肌细胞凋亡率，ELISA法检测心肌细胞上清TGFβ1蛋白浓度。结果：原代分离培养第3天的心肌细胞纯度可达90%。多柔比星在0.5～2 μmol/L浓度范围内，随着处理时间延长，心肌细胞存活率下降，呈剂量和时间依赖性；2 μmol/L多柔比星可诱导心肌细胞凋亡，在4～48 h内细胞凋亡率不断增加，且心肌细胞TGF-β1蛋白表达量也逐渐增加，与凋亡率呈正相关。结论： TGF-β1可能在多柔比星诱导的大鼠心肌细胞凋亡中起促进作用。

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	陈星
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	张国辉

关键词 ：转化生长因子-&beta, 1, 多柔比星, 心肌细胞, 细胞凋亡

Abstract：Objective: To investigate the effect of TGF-β1 on the apoptosis of cardiomyocytes induced by doxorubicin(DOX). Methods: The cardiomyocytes isolated from 1~2 d SD rats were cultured in DMEM-F12 medium. The purify of cardiomyocytes was evaluated by immunocytochemical staining with α-sarcomericactin. The third day cultured cardiomyocytes were randomly divided into control group and DOX groups.The cardiomyocytes of DOX groups were treated by different concertrations of DOX(0.5,1,2 μmol/L) for different times(4,12,24,48,72 h）. The survival rate of cardiomyocytes was evaluated by MTT. The apoptosis rate was determined by flow cytometry (FCM）. The expression of TGF-β1 in the cardiomyocytes was detected by ELISA. Results: The purify of the third daycultured cardiomyocytes was above 90%.In a certain range of concertration(0.5~2 μmol/L）, DOX could inhibit the growth of cardiomyocytes in a dose and time manner. 2 μmol/L DOX could significantly induce the apoptosis of cardiomyocytes.During the time point 4 h to 48 h,the apoptosis rate was increased, while the expression of TGF-β1wasalso increased and positively correlated with the former. Conclusion: The increasing expression of TGF-β1 might contribute to the apoptosis of rat cardiomyocytes induced by doxorubicin.

通讯作者: 张国辉（通讯作者），主任医师，硕士生导师，E-mail: 13338812776@189.cn

作者简介: 陈星（1986—），女，硕士研究生

引用本文:

陈星，潘建业，周艳芳，王好，顾琳，张国辉. 转化生长因子-β1在多柔比星致大鼠心肌细胞凋亡中的促进作用[J]. 江苏大学学报:医学版, 2012, 22(1): 46-.

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http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2012/V22/I1/46

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