Recombinant Newcastle disease virus (rLRVG) triggers apoptosis by inducing endoplasmic reticulum stress in gastric carcinoma cell
ZHAO Ying-hai 1,2, YAN Yu-lan 3, LI Mi 1,2
（1. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2. Department of General Surgery, Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002; 3. Department of Respiratory Medicine, Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002, China)
Abstract：Objective: To investigate the effects of newcastle disease virus (NDV) and recombinant avirulent NDV LaSota strain expressing the rabies virus glycoprotein(rL-RVG) on endoplasmic reticulum stress and apoptosis of HGC cells. Methods: The cells were divided into three groups at random, control group, NDV group, and rL-RVG group, which infected with PBS, NDV and rL-RVG, respectively. The expression of endoplasmic reticulum stress related proteins, glucoseregulated protein 78(GRP78),CCAAT/enhancerbinding protein homologous protein(CHOP) were analyzed by western blotting and immunofluorescence. The HGC cells were divided into four groups at random, control group, 4-PBA group, rL-RVG group and 4-PBA+rL-RVG group. 4-PBA group and 4-PBA+rLRVG group were treated with sodium phenylbutyrate(4-PBA) for 4 h, then rL-RVG group and 4-PBA+rL-RVG group were infected with rL-RVG, while rL-RVG were replaced with PBS in control group and 4-PBA group. The expression of caspase-3 protein was detected with western blotting. The rate of cell early apoptosis were detected by flow cytometry. Results: After infected 24 hours, compared with the control group, NDV group and rL-RVG group had high levels of GRP78 and CHOP protein expression(P<0.05), and rL-RVG group showed higher levels than NDV group(P<0.01). The expression of GRP78, CHOP, caspase-3 protein and the rate of apoptosis in 4-PBA+rL-RVG group was lower than rL-RVG group after pretreated with 4-PBA (P<0.05). Conclusion: The rL-RVG could induce stronger endoplasmic reticulum stress and related apoptosis than the NDV.
2016, Vol. 26 
