Expressions of lncRNA aHIF and ANRIL in gastric carcinoma
LIU Xin1, ZHANG You-li1, QIN Yan1, NI Xin1, GONG Ai-hua2, ZHANG Xing-xing1,
（1. Department of Gastroenterology, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001; 2. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013, China)
Abstract：[Abstract]Objective: To explore the expression level of antisense hypoxia inducible factor (aHIF) of long noncoding RNA (lncRNA) and antisense noncoding RNA (ANRIL) which located in cell cycle kinase inhibitory factor 4 (INK4) loci in gastric cancer tissue and plasma. Methods: We collected 20 cases gastric cancer tissues and the normal tissue adjacent to carcinoma of gastric cancer patients, 33 cases plasma of patients with gastric carcinoma and 20 cases plasma of healthy subjects. Realtime fluorescence quantitative PCR was used to determine the mRNA expression levels of lncRNA aHIF and ANRIL; the relationship between the two mRNA expressions in plasma and tissues of gastric cancer patients were analyzed. Results: The mRNA expression levels of lncRNA aHIF and ANRIL in gastric cancer tissue were obviously higher than those of adjacent carcinoma tissues (P<0.01). Compared with the healthy subjects, the mRNA expression of aHIF and ANRIL in plasma of gastric cancer patiens was significantly increased (P<0.01), which positively related to the mRNA expression of gastric cancer tissues(r=0.536,0.524, P<0.05). Conclusion: LncRNA aHIF and ANRIL were overexpression in tissue and plasma of gastric cancer patients.
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2016, Vol. 26 
