IL-23 strengthened epithelialmesenchymal transition and migration of esophageal squamous carcinoma cells
HAN Guo-hu1, LIU Shen-zha1, LI Wei2, WANG Xue-feng3, ZHOU Yue-peng3, MAO Chao-ming3, CHEN De-yu1
（1. Department of Oncology, Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001; 2. Department of Oncology, Jintan Hospital Affiliated to Jiangsu University, Changzhou Jiangsu 213200; 3. Department of Nuclear Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001, China)
Abstract：[Abstract]Objective: To explore the effect of exogenous IL-23 through PI3K/AKT/cMyc pathway on the epithelialmesenchymal transition (EMT) and migration of esophageal squamous carcinoma cells. Methods: The expression and localization of IL-23 and interleukin23 receptor (IL23R) in tumor and adjacent normal tissues from 12 esophageal squamous cell carcinoma (ESCC) patients were detected by immunohistochemistry and immunofluorescence; IL-23p19 and IL-23R expression in TE-1 cells and NIH3T3 cells was detected by Western blotting and flow cytometry respectively; PCR and Western blotting were used to examine the expression of c-Myc mRNA and c-Myc,E-cadherin,Vimentin,p-AKT,Snail 1,Slug protein in control group, 50 ng/mL IL23 group, and 1 μmol/L Wortmannin +50 ng/mL IL-23 group in TE-1 cells; and the invasion and migration of IL-23 treated TE1 cells was evaluated by Transwell assay and wound healing assay. Results: Compared with adjacent normal tissues, IL-23 highly expressed in ESCC tissues and cytoplasm, and IL-23R also expressed on the cell membrane. Compared with negative control NIH3T3 cells, IL-23p19 and IL-23R were showed higher expression levels in TE1 cells(P<0.01). Compared with control group, the expression of c-Myc mRNA, c-Myc, p-AKT, Vimentin, Snail 1, Slug protein levels were upregulated, E-cadherin protein was down-regulated, the rate of cell wound healing and the migration were significantly increased in IL23 group (all P<0.05). Compared with IL-23 alone group, the expression of c-Myc mRNA and c-Myc, p-AKT, Vimentin, Snail 1, Slug protein levels were downregulated, E-cadherin protein was up-regulated, the rate of cell wound healing and the transfer ability were significantly reduced in IL-23+ Wortmannin group (all P<0.05). Conclusion: IL-23 strengthened EMT and migration of esophageal squamous carcinoma cells via PI3K/AKT/cMyc pathway.
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2016, Vol. 26 
