Effect of PECAM-1 siRNA on cell proliferation and apoptosis of irradiation-induced drug resistance CNE1/R cells of human
nasopharyngeal carcinoma
WEI Ji-lou1, LIU Fang2, JIANG Zhong-xiu3, CHENG Hong-lei2, LUO Su-mei2，WANG Yue-li2, WANG Ruo-yu2
(1.ICU, Huai′an First People′s Hospital, Nanjing Medical University, Huai′an Jiangsu 223300; 2.Department of Oncology, the Affiliated Zhongshan Hospital of Dalian University, Dalian Liaoning 116001; 3.Department of Oncology, Shengjing Hospital of China Medical University, Shenyang Liaoning 110004, China)
Abstract：[Abstract]Objective: To investigate the effect of silencing platelet-endothelial cell adhesion molecule-1(PECAM-1) by small interfering RNA (siRNA) on cell proliferation and apoptosis of irradiation-induced drug resistance CNE1/R cells of human nasopharyngeal carcinoma. Methods: The CNE1/R-specified siRNA was designed and synthesized. The purity of the transfected cells were screened out by G418. The expression of PECAM-1 was detected by western blotting. Cell growth and apotosis was assayed by MTT and flow cytometry, respectively. Results: The expression of PECAM-1 protein in CNE1/R cells was significantly downregulated. Compared with negative group and control group, experiment group showed significantly decreased proliferation ability in CNE1/R cells, whereas cell apoptosis rate was increased. Conclusion: PECAM-1 siRNA could inhitit the growth and promote the apoptosis of CNE1/R cell, which suggested PECAM-1 might play a key role in CNE1/R cell growth and apoptosis.
魏继楼1， 刘芳2， 蒋中秀3， 程红蕾2， 罗素梅2， 王月丽2， 王若雨2 . PECAM-1 siRNA对人鼻咽癌辐射致耐药CNE1/R细胞增殖与凋亡的影响[J]. 江苏大学学报:医学版, 2013, 23(5): 393-396,401.
WEI Ji-lou1, LIU Fang2, JIANG Zhong-xiu3, CHENG Hong-lei2, LUO Su-mei2，WANG Yue-li2, WANG Ruo-yu2.
Effect of PECAM-1 siRNA on cell proliferation and apoptosis of irradiation-induced drug resistance CNE1/R cells of human
nasopharyngeal carcinoma. Journal of Jiangsu University(Medicine Edition), 2013, 23(5): 393-396,401.
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2013, Vol. 23 
