Abstract：Objective: Human acute promyelocytic leukemia (APL)cell line NB4 was used as a model to discuss whether arsenic trioxide (ATO) can inhibit the expression of Hedgehog signaling pathway molecules，so that we can provide a theoretical basis for the application of ATO in other cancers with an aberrant activation of Hedgehog signaling pathway. Methods： Treated with ATO of different concentration, the expression of Hh pathway components such as Smo,Ptch,Gli1 and Gli2 were analyzed with real time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western Blot in NB4 at mRNA and protein level.Results： After ATO treatment, the expression of Smo, Ptch and Gli2 were significantly downregulated in NB4. Conclusion: ATO may inhibit the expression of Hh signaling pathway through the mRNA and protein inhibition of the key Gli2 target transcription factor and ATO may inhibit APL partly through the inhibition of Hedgehog signaling pathway.
收稿日期: 2013-04-25
基金资助:
黑龙江省青年科学基金资助项目（QC2009C27）
通讯作者:
周晋（通讯作者），教授，E-mail:zhoujin1111@yeah.net
作者简介: 杨东光（1978—），女，黑龙江伊春人，博士后，助理研究员；
引用本文:
杨东光， 曹峰林， 叶向梅， 赵辉， 李洋， 王洪玲， 周晋. 三氧化二砷对急性早幼粒细胞白血病细胞株Hedgehog信号通路的影响[J]. 江苏大学学报:医学版, 2013, 23(5): 389-392.
YANG Dong-guang, CAO Feng-lin, YE Xiang-mei, ZHAO Hui, LI Yang, WANG Hong-ling, ZHOU Jin. Inhibition of arsenic trioxide on Hedgehog pathway in acute promyelocytic leukemia cell line. Journal of Jiangsu University(Medicine Edition), 2013, 23(5): 389-392.
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2013, Vol. 23 
