目的:  探讨白细胞介素36γ（IL-36γ）是否能够增强体外培养的人CD8⁺T淋巴细胞分泌γ干扰素。方法: 经密度梯度离心法从健康人外周血分离获得外周血单个核细胞（PBMC），经磁珠阳性选择富集纯化CD8⁺T淋巴细胞，分别用CD3单克隆抗体（mAb）+CD28 mAb，CD3 mAb+CD28 mAb+IL-12，CD3 mAb+CD28 mAb+IL-36γ以及CD3 mAb+CD28 mAb+IL-12+IL-36γ 4种因子组合刺激培养24 h和72 h。收集细胞，采用免疫荧光标记和流式细胞术分析分选富集的CD8⁺T淋巴细胞纯度以及γ干扰素的表达；采用实时PCR检测各组细胞IL-36受体（IL-36R）、γ干扰素和颗粒酶B mRNA的表达。结果：  人CD8⁺T淋巴细胞表达IL-36R；各组因子刺激的人CD8⁺T淋巴细胞形态没有明显差异；人CD8+T淋巴细胞经IL-36γ刺激后，γ干扰素、颗粒酶B mRNA表达上调；同时， IL-36γ和IL-12 具有协同刺激CD8⁺T淋巴细胞的作用。结论： IL-36γ能促进体外培养的人CD8⁺T淋巴细胞分泌细胞因子，并且与IL-12具有协同作用。

Objective: To investigate whether recombinant interleukin-36γ(rIL-36γ) can enhance human CD8⁺T lymphocytes secrete cytokine interferon-γ（IFN-γ）in vitro. Methods: Peripheral blood mononuclear cells(PBMC) was isolated from healthy donors by density gradient centrifugation,and CD8⁺T lymphocytes were purified by positive magnetic beads selection from PBMCs. CD8+T cells were stimulated with different combination of cytokines(CD3 mAb+CD28 mAb；CD3 mAb+CD28 mAb+IL-12；CD3 mAb+CD28 mAb+IL-36γ；CD3 mAb+CD28 mAb+IL-12+IL-36γ) in vitro. The cells of each group were collected after 24 h and 72 h. CD8+T lymphocytes purity and cytokine IFN-γ production were determined by flow cytometry. Real time PCR(qRT-PCR) was used to detect IL-36 receptor(IL36R), IFNγ and granzyme B mRNA expression. Results: Human CD8+T lymphocytes expressed IL36 receptor；there was no significant difference of cell morphology among the four groups of CD8+T lymphocytes；IFN-γ and granzyme B of CD8⁺T lymphocytes stimulated by IL-36 γ were significantly increased in mRNA levels(P<0.05)；at the same time, IL-36γ and IL-12 had a costimulation role to activate CD8⁺T lymphocytes. Conclusion: IL-36γ has a synergistic effect with IL-12 to enhance human CD8⁺T lymphocytes cytokine secretion in vitro.