一种用于过氧亚硝酸根检测的近红外比率荧光探针的研制

韩志湘; 代晓婷; 熊杰

doi:10.3969/j.issn.1671-7775.2024.01.016

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江苏大学学报（自然科学版） ›› 2024, Vol. 45 ›› Issue (1) : 112-118. DOI: 10.3969/j.issn.1671-7775.2024.01.016

环境与土木工程

一种用于过氧亚硝酸根检测的近红外比率荧光探针的研制

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A near-infrared region ratiometric fluorescent probe for peroxynitrite detection

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摘要

设计合成了一种基于半花菁的近红外比率荧光探针分子Cy-P，并将其用于活细胞中的外源性和内源性过氧亚硝酸根（ONOO-）荧光成像检测.研究结果表明：探针分子Cy-P自身具有较大的π共轭结构，其最大发射波长在近红外区域；加入ONOO-后，强氧化性的ONOO-会导致Cy-P的π共轭体系被破坏，并生成蓝色荧光物质，最大发射波长蓝移了268 nm；探针分子Cy-P对ONOO-线性检测范围为0~15 μmol/L，检测下限为13 nmol/L；该探针分子对ONOO-的识别性能优于其他各种可能干扰的生物分析物；该探针分子具有低细胞毒性，适用于活细胞中的外源性和内源性ONOO-的成像检测.

Abstract

A near-infrared region (NIR) ratiometric fluorescent probe Cy-P based on hemicyanine was rationally designed, prepared and utilized for the detection of exogenous and endogenous peroxynitrite（ONOO-） in living cells. The research results show that Cy-P itself displays NIR emission owing to the large π-conjugated system. With the addition of the strong oxidizer ONOO-, the π-conjugated system of Cy-P is destroyed, and a new substance with blue fluorescence is formed, leading to 268 nm blueshift of fluorescent emission. The linear detection range of the probe Cy-P to ONOO- is 0-15 μmol/L, and the detection limit is 13 nmol/L. The probe exhibits superior selectivity for ONOO- over various potentially interfering biological analytes. Due to the low cytotoxicity, the prepared probe is suitable for imaging detection of exogenous and endogenous ONOO- in living cells.

导出引用

韩志湘, 代晓婷, 熊杰.

一种用于过氧亚硝酸根检测的近红外比率荧光探针的研制

[J]. 江苏大学学报（自然科学版）, 2024, 45(1): 112-118 https://doi.org/10.3969/j.issn.1671-7775.2024.01.016

HAN Zhixiang, DAI Xiaoting, XIONG Jie. A near-infrared region ratiometric fluorescent probe for peroxynitrite detection[J]. Journal of Jiangsu University(Natural Science Edition), 2024, 45(1): 112-118 https://doi.org/10.3969/j.issn.1671-7775.2024.01.016

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