人神经干细胞源施万样细胞微囊泡促进大鼠神经元轴突生长

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Abstract Objective: To explore the effect of microvesicles of Schwannlike cells derived from human neural stem cells on axon growth of neurons. Methods: Dorsal root ganglion(DRG) neurons were obtained from DRG of suckling rats born 4-5 d. DRG neurons were stimulated by microvesicles of Schwannlike cells, and immunofluorescence staining of βⅢ-tubulin was performed to observe the axon growth. After stimulating neuronlike N2a cells with 0, 5, 20, 40 mg/L microvesicles of Schwann-like cells for 19 h, the expression of growth associated protein 43(GAP43) mRNA in N2a cells was detected by realtime PCR. After stimulating neuron-like N2a cells with 0, 20, 40 mg/L microvesicles of schwann-like cells for 48 h, periodic acidSchiff(PAS) staining was performed to detect intracellular glycogen production. Results: The axon length of DRG neurons in the Schwann cell microvesicle group was significantly longer than that in the PBS group(P<0.05). Microvesicles of Schwann-like cells could be internalized by N2a cells, and there was no significant difference in GAP43 mRNA expression between 5 mg/L group and 0 mg/L group(P<0.05). Compared with 0 mg/L group, 20 and 40 mg/L groups showed higher levels of GAP43 mRNA expression, neurite outgrowth rate and PAS staining positive cell rate (all P<0.05).Conclusion: Microvesicles of Schwannlike cells derived from human neural stem cells could promote the growth of murine axons in vitro.

Received: 19 March 2019

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2019/V29/I04/322

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