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Effect of heat shock protein family A member 5(HSPA5) on
artesunateinduced ferroptosis in hepatic carcinoma |
WANG Kang1, ZHANG Zheng-yang2, SONG Lian2, GONG Ai-hua1, WANG Dong-qing2, ZHU Hai-tao2 |
(1. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2. Central Laboratory of Radiology, Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001, China)
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Abstract Objective: To investigate the effect of heat shock protein family A member 5(HSPA5) on the chemosensitivity of artesunate in human hepatic carcinoma SMMC7721 cells. Methods: SMMC7721 cells were treated with different concentrations of artesunate, CCK8 assay was used to detect the cell viability, and to screen the best conecntration. SMMC-7721 cells were divided into control group, artesunate group, artesunate+deferaxamine group. CCK8 assay was used to test the cell viability. Flow cytometry was used to detect the level of lipid reactive oxygen species(ROS). The malondialdehyde detection kit was used to detect the level of malondialdehyde. qRT-PCR and Western blotting were used to detect the mRNA and protein expression level of HSPA5 in SMMC-7721 cells treated with Vector, HSPA5-shRNA or Flag-HSPA5 plasmid. The CCK-8 assay kit was used to test the cell viability. The malondialdehyde detection kit was used to detect the level of malondialdehyde. Results: The cells showed the modest survival rate when the concentration of artesunate was 20 μmol/L. Flow cytometry showed the levels of lipid ROS and malondialdehyde in cells treated with artesunate increased(P<0.05), which could be reversed by deferoxamine; cell viability in the HSPA5-shRNA group treated with artesunate were lower than those in the Vector group(P<0.05). The level of malondialdehyde in the HSPA5shRNA group treated with artesunate were higher than those in the Vector group(P<0.05). Conclusion: Knockdown of HSPA5 may enhance the chemosensitivity of artesunate in human hepatic carcinoma SMMC7721 cells.
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Received: 05 March 2019
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