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Construction of recombinant oncolytic vaccinia virus armed with anti-FAP and anti-CD3 bi-specific antibody and its anti-tumor activity |
| SHI Jin-sheng1, LI Feng1, WU Si-hui2, YU Li-chao1, LIU Li-qiong3,YUFeng4,5 |
| (1. Department of Cardiothoracic Surgery,Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001; 2. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 3. Department of Hematology,Shenzhen Nanshan Hospital, Shenzhen Guangdong 5180052; 4. Institute of Life Sciences, Jiangsu University,Zhenjiang Jiangsu 212013; 5. the State Key Laboratory of Oncogenes and Related Genes,Shanghai 200032, China)
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Abstract Objective: To construct double deleting recombinant oncolytic vaccinia virus armed with bi-specific antibody of antifibroblast activation protein(FAP) and anti-mouse CD3(BiTE.FAP), and to evaluate the anti-tumor efficacy in vitro. Methods: The BiTE-FAP gene regulated by the F17R promoter(PF17R) and the DsRed gene regulated by the SE/L promoter(PSEL) were homologously recombinated into the TK locus of the vSC20 parental virus to construct the recombinant double deleted vaccinia virus-bispecific T cell engager-FAP(rVVDD- BiTE.FAP).Plaque counting was used to assess the replication ability of recombinant oncolytic virus. MTS and LDH assay were used to detect the inhibitory effect on mouse lung tumor cells.ELISA assay was performed to analyse the IL-2 and IFN-γ expression by mouse spleen T cells. Results: rVVDD-BiTE.FAP was obtained by homologous recombination and soft agar purification.MTS and crystal violet staining results showed that rVVDD-BiTE.FAP has the same replication capacity compared with the parental vSC20 virus and mouse T cells could enhance the anti-tumor effect of rVVDD-BiTE.FAP. Conclusion: rVVDD-BiTE.FAP has high anti-tumor efficacy.
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2019, Vol. 29 
