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| Co-cultured with primary chondrocytes promotes differentiation of BMSCs into chondrocytes |
| FENG Xiao1, HAN Xu1, ZHANG Ya1, HU Bin2, HUANG En-fu1, WANG Xiao-dong2 |
| (1. Institute of Pediatrics Research, 2. Department of Orthopaedics, Childrens Hospital of Soochow University, Suzhou Jiangsu 215003, China) |
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Abstract Objective: By coculturing the mixture of the bone marrow mesenchymal stem cells(BMSCs) with primary articular chondrocytes(AC) coaggulated in the centrifuge tube,efficacy of chondrocytes induction was compared between coculture and chondrocytesonly culture. Methods: After BMSCs and AC were isolated from the marrow and cartilage of New Zealand white rabbits, BMSCs and AC were cultured in vitro separately. Alcian blue, safraninO staining and type Ⅱ collagen immunohistochemical staining were applied to identify chondrocytes. Expressions of CD44, CD105, CD34 and CD45 were immunofluorescencally assessed to identify BMSC. After culturing, the primary chondrocytes were mixed with the third generation of BMSCs by 1 ∶3 in the centrifugal tube, as coculture group. Meanwhile, the pure AC cultured as the control, as ACalone group. Cell supernatants were collected weekly for 4 weeks and glycosaminoglycan(GAG) and type Ⅱ collagen in two groups were determined. Results: There was no significant difference of gross morphology and histological observation in two groups.The ability of secreting GAG and type Ⅱ collagen were also similar in two groups(P>0.05). Conclusion: coculturing BMSCs with chondrocytes in the centrifuge tube could promote induction of BMSC differentiating into AC successfully.
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Received: 17 May 2017
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2017, Vol. 27 
