丝氨酸蛋白酶抑制物vaspin对缺氧/复氧诱导的心肌细胞凋亡的影响

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Abstract Objective: To investigate the effects and the possible underlying mechanisms of vaspin on the apoptosis of cardiomyocytes induced by hypoxia/ reoxygenation (H/R) injury in vitro. Methods: Isolated rat cardiomyocytes were divided into the following groups: control group, H/R group, and H/R + vaspin group (300 ng/mL). The apoptosis of cardiomyocytes were detected by TUNEL method, and the expressions of phosphatidylinositol 3 kinase(PI3K) and Akt in the cardiomyocytes were evaluated by Western blotting. Results: Hypoxia/reoxygenation injury increased apoptosis, but this effect was protected by vaspin. In addition, vaspin also promoted the expression of PI3K p100γ, Akt1, Akt and pAkt in the cardiomyocytes treated with hypoxia/ reoxygenation. Conclusion: Exogenous vaspin could activate the PI3K/Akt signaling pathways in cardiomyocytes and protected the apoptosis of cardiomyocytes induced by hypoxia/reoxygenation injury.

Received: 19 May 2017

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2017/V27/I5/408

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