透明质酸功能化三氧化二铋纳米颗粒的制备及其对肝癌细胞的放疗增敏作用

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Abstract Objective: To investigate the radiosensitization enhancements of a newly developed nanopartical modified by hyaluronic acid (HABi2O3) on hepatoma cells SMMC7721. Methods: We used HA and BiCl3·6H2O as precursors to synthesize HABi2O3 by polyol method. HABi2O3 were characterized by transmission electron microscopy(TEM/HRTEM), NanoDLS particle size analyzer and Xray diffraction(XRD).The biocompatibility characterization of HABi2O3 NPs was characterized by CCK8 assay, histological analysis and inductively coupled plasma mass spectrometry(ICPMS). The radiosensitization of HABi2O3 was conducted by CCK8 assay,Livedead staining assay(FDAPI staining) and clonogenic assay, randomly on SMMC7721 cells, divided to four groups (control, HABi2O3, radiation and HABi2O3+radiation). Results: We fabricated the HABi2O3 with a diameter of 45 nm,the amorphous phase structure and these diffraction peaks of HABi2O3 NPs matched well with the characteristic peaks of cubic Bi2O3 (JCPDS 2004 060312). Inhibition rates of cell proliferation of HABi2O3 ranging with different concentrations of HABi2O3(0-400 mg/L) were no statistically significant differences(P＞0.05). HABi2O3 was no obvious toxicity to the mouse susceptible organs (liver, heart, spleen, lung, and kidney) showed by histological assessment and ICPMS. Compared with control, radiation group and HABi2O3 group, there were lower inhibition rates of cell proliferation and survival fraction in HABi2O3+radiation group with statistically significant differences(P＜0.05). Conclusion: We have successfully developed HABi2O3 with favorable solubility in water, excellent biocompatibility, no obvious toxicity and effective radiosensitization enhancement on hepatoma SMMC7721 cells.

Received: 17 March 2017

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2017/V27/I5/397

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