Effects of α-lipoic acid on liver oxidative stress and endoplasmic reticulum stress in type 2 diabetic rats
LI Jing1, LI Rui-fang2 , WANG Guo-xian1, LI Yun2
(1.Department of Pharmacology，Liaoning Medical College, Jinzhou Liaoning 121001；2.Department of Cerebral Surgery, the Hospital of Chinese Medicine of Handan，Handan Hebei 056001, China）
Abstract：Objective: To investigate the protective effects of α-lipoic acid(A-LA) on liver in type 2 diabetic rats；the JNK and p-JNK protein expression related to ERS of liver in 2 diabetic rat. Method：Type 2 diabetes mellitus models were induced by highfat diet and intraperitoneal injection of streptozotocin in fifty male Sprague Dawley rats. then divided into three groups: diabetic group, A-LA group and Vit E group.The groups except diabetic group treated with A-LA and VitE by intragastric for twelve weeks. The fasting glucose，blood lipid and serum liver function indices were measured after twelve weeks. Apoptosis was detected by TdT-mediated dUTP nick end labeling；Western blot analysis was employed to detect liver Phospho-c-Jun／JNK protein expression. Results: Compared with the control groups, diabetes model rats blood sugar and TG,TC,AST,ALT and MDA increased significantly (P<0.05), SOD and GSH-Px reduced significantly (P<0.05), the rate of cell apoptosis was increased. Protein expression of JNK didn′t not change but phosphorylated JNK were significantly up (P<0.05). Compared with the diabetic group, A-LA groups above all indexes had been improved, the activities of SOD and GSH-Px were improved; the rate of cell apoptosis was reduced, protein expression of p-JNK was down. Vit E group blood sugar was not changed,but the SOD and GSH-Px increased significantly. Conclusion： Alpha lipoic acid has a protective effect on liver in diabetic rats, the mechanism may be related to the inhibition of oxidative stress and endoplasmic reticulum stress
收稿日期: 2012-10-08
通讯作者:
王国贤教授，硕士生导师，E-mail：wangguoxian2007@sohu.com
作者简介: 李静（1980—），女，硕士研究生
引用本文:
李静1，李瑞芳2，王国贤1，李云2. α-硫辛酸对2型糖尿病大鼠肝脏氧化应激和内质网应激的影响[J]. 江苏大学学报:医学版, 2013, 23(1): 5-8.
LI Jing1, LI Rui-fang2 , WANG Guo-xian1, LI Yun2.
Effects of α-lipoic acid on liver oxidative stress and endoplasmic reticulum stress in type 2 diabetic rats. Journal of Jiangsu University(Medicine Edition), 2013, 23(1): 5-8.
2013, Vol. 23 
