Abstract：[Abstract] Objective: To study the percentage of CD4+CD25+Foxp3+, CD4+IL-17+ cells and the expression Foxp3, IL-17, RORγt mRNA in peripheral blood from patients with asthma. Methods： The specimens of peripheral blood mononuclear cell(PBMC) were collected from 23 patients of asthma in remission stage, 25 patients of acute paroxysmal asthma and 20 healthy persons(control group). Intracellular flow cytometry detection of the percentage of CD4+CD25+Foxp3+ cells and CD4+IL-17+ cells was established after isolation of untouched human CD4+T cells from PBMC. The expression level of Foxp3, IL-17, RORγt mRNA in CD4+T cells was determined by realtime fluorescence quantitative RT-PCR. Results： Immunomagnetic bead shade selected CD4+T cells reached a purity of more than 90%. The percentage of CD4+CD25+Foxp3+ cells and the expression level of Foxp3 mRNA of the acute paroxysmal group was significantly lower than that of the control group and remission group(P＜0.01), and the comparison of the control group and remission group didn＇t have significant deviation(P＞0.05). The percentage of CD4+IL-17+ cells and the expression level of IL-17、RORγt mRNA of the acute paroxysmal group was significantly higher than those of the control group and remission group (P＜0.01),and the expression level of IL-17、RORγt mRNA in the remission group was also significantly higher than the control group(P＜0.05). Conclusion：The percentage of CD4+CD25+Foxp3+cells and the expression of Foxp3 mRNA in peripheral blood from patients with asthma decreased significantly.The percentage of CD4+IL-17+cells and the expression of IL-17、RORγt mRNA in peripheral blood from patients with athma increased significantly.The imbalance of Treg/Th17 perhaps correlated with the acute episode of asthma.
收稿日期: 2012-10-11
基金资助:
江苏大学临床医学科技发展基金资助项目（JLY2010121）
作者简介: 荣磊（1974—），男，安徽界首人，硕士研究生，主治医师，主要从事呼吸疾病的临床研究。
引用本文:
荣磊，马国尔，卞秀娟. 哮喘患者外周血调节性T细胞和Th17细胞数量及其转录因子的变化[J]. 江苏大学学报:医学版, 2012, 22(6): 530-533.
RONG Lei, MA Guo-er, BIAN Xiu-juan. Analysis of Th17 and Treg cells and corresponding transcription factor in peripheral blood from patients with asthma. Journal of Jiangsu University(Medicine Edition), 2012, 22(6): 530-533.
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2012, Vol. 22 
