Effect of Kecuan granule on Th1/Th2 cells in peripheral
blood from children with hot asthma
WANG Hai-sheng1， WANG Wu-qiang2, LIU Xiao-lin1, XUE Jian-hua1, WU Yu-fang1, MA Mei-xiang1
(1. Department of Clinical Laboratory, 2. Department of Chinese Pediatrics, Wujin Hospital of Traditional Chinese Medicine, Changzhou Jiangsu 213161, China)
Abstract：Objective: To explore the effect of Kecuan granule on Th1/Th2 cells in peripheral blood from children with hot asthma. Methods: Fortyeight children with hot asthma were randomly divided into granule group and routine group, 24 cases in each group. The granule group received the administration of kecuan granule, three times per day, 1 course of 3-d treatment, for 2 courses; and the routine group received the administration of routine western medicine, pulmicort turbuhaler, 10 μg/inhaling, and terbutaline sulfate aerosol, 0.25mg/spray, twice per day, for one week. Additionally, 20 healthy children served as controls. The mRNA levels of T-box expressed in T cells (T-bet) and GATA binding-3 (GATA-3) in human peripheral blood mononuclear cells (PBMC) were detected by quantitative realtime PCR and the levels of interferon-γ(IFN-γ) and IL-4 in plasma were detected by ELISA. Results: Total effective rates of the granule group and routine group were 95.83% and 91.67%, respectively, showed no significant differences (χ2=0.559, P>0.05). Before treament, the levels of GATA3 mRNA and IL-4 in the granule group and routine group were notablely higher than those in the control group (P0.05), but the ratios of T-bet mRNA/GATA-3 mRNA and IFN-γ/IL-4 in the granule group and routine group were significantly lower than those in the control group (P0.05), but in the granule group and routine group, the levels of GATA-3 mRNA and IL-4 were markedly lower than before treatment (P0.05). Conclusion: Children with hot asthma showed Th2 accentuation and the imbalance of Th1/Th2, which could be cured by kecuan granule through inhibiting Th2 cells and correcting the imbalance of Th1/Th2.
收稿日期: 2014-03-20
基金资助:江苏省常州市武进区科技项目（WS201231）
作者简介: 王海生(1971—)，男，江苏扬州人，硕士，副主任技师，主要从事免疫调节方面的研究。
引用本文:
王海生1， 王武强2， 刘小林1， 薛建华1， 吴玉芳1， 马梅香1. 咳喘颗粒剂对热型哮喘患儿外周血Th1/Th2细胞的影响[J]. 江苏大学学报:医学版, 2014, 24(05): 408-411，415.
WANG Hai-sheng1， WANG Wu-qiang2, LIU Xiao-lin1, XUE Jian-hua1, WU Yu-fang1, MA Mei-xiang1. Effect of Kecuan granule on Th1/Th2 cells in peripheral
blood from children with hot asthma. Journal of Jiangsu University(Medicine Edition), 2014, 24(05): 408-411，415.
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2014, Vol. 24 
