The effect of epigallocatechin3gallate on LRRK2 activity and its mechanism
NIU Jing-xin1, GUO Jing2, GUO Qing1, LI Jie3, LIU Jun1, LIU Zhao-hui1
(1. Department of Human Anatomy & Histology and Embryology, Medical School of Soochow University, Suzhou Jiangsu 215123; 2. Department of Preclinical Medicine, Lianyungang Higher Vocational Technical College Traditional Chinese Medicine, Lianyungang Jiangsu 222007; 3. Department of Preclinical Medicine, Suzhou Vocational Health College, Suzhou Jiangsu 215009, China)
Abstract：Objective: To study the effect of epigallocatechin3gallate (EGCG) on leucine rich repeat kinase 2 (LRRK2) activities in Drosophila model and its potential molecular mechanism. Methods: DdcLRRK2G2019S strains of Drosophila was selected and divided into 7 groups. The control group was fed with standard corn medium. The Drosophila of each experimental group were fed with medium containing GW5074, NAC and EGCG with concentrations of 0.1, 1, 10 and 100 μmol/L, respectively. The life span and motor function of Drosophila were tested and screened for the optimal concentration and optimal time of drug action.Western blotting was used to detect pLRRK2, pp38 MAPK, nuclear factor erythroid 2related factor 2(Nrf2), pERK1/2 and tyrosine hydroxylase(TH) protein expressions in Drosophila brain tissues. Results: Compared with the control group, both 10 μmol/L EGCG group and 1 μmol/L EGCG group had significantly prolonged the lifespan and improved the motor function of Drosophila(P<0.05), while the effect of 0.1 μmol/L EGCG and 100 μmol/L EGCG group was not significant; there were marked differences in the behavioral changes of Drosophila in the 5th week(P<0.05). Compared with the control group, 10 μmol/L and 1 μmol/L EGCG group showed significantly higher expression of Nrf2, pERK1/2 and lower expression of pLRRK2, pp38 MAPK(P<0.05). Conclusion: EGCG might effectively inhibit LRRK2 kinase activity through signaling pathways such as Keap1Nrf2ARE and MAPK.
钮婧歆1， 郭晶2， 郭青1， 李杰3， 刘珺1，*， 刘朝晖1，*. EGCG对富亮氨酸重复激酶2活性的影响及其作用机制[J]. 江苏大学学报:医学版, 2018, 28(05): 397-402.
NIU Jing-xin1, GUO Jing2, GUO Qing1, LI Jie3, LIU Jun1, LIU Zhao-hui1. The effect of epigallocatechin3gallate on LRRK2 activity and its mechanism. Journal of Jiangsu University(Medicine Edition), 2018, 28(05): 397-402.
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2018, Vol. 28 
