Expression of paxillin in cervical cancer and its prognostic value
LI Juan1, LIU Qiu-li2, JIA Dai-lin3, LI Jing4, QIU Hai-feng5, WANG Yuan3, YANG Pei-fang6
（1. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2. School of Medicine, Jiangnan University, Wuxi Jiangsu 214122; 3. Department of Gynecologic Oncology, the Affiliated Hospital of Jiangnan University, Wuxi Jiangsu 214062; 4. Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou Henan 450052; 5. Department of Gynecologic and Obstetrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou Henan 450052; 6. Department of Gynecologic and Obstetrics, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001, China)
Abstract：Objective: To investigate the expression of paxillin in cervical cancer tissues and its prognostic value in cervical cancer patients. Methods: Immunohistochemistry staining was used to detect the expression of paxillin in 150 cervical cancer tissues, 62 cervical intraepithelial neoplasia tissues and 50 normal cervical squamous epithelium tissues. The correlation of paxillin expression in cervical cancer tissues with the clinicopathological factors was analyzed. Results: The positive rate of paxillin expression in cervical cancer(125/150，83.33%) was significantly higher than that in cervical intraepithelial neoplasia tissues(19/62，30.65%) and normal cervical squamous epithelium tissues(14/50，28.00%, P<0.01). The expression of paxillin was significantly correlated to serum carcinoembryonic antigen level(P=0.04), serum CA125 level(P=0.013), and distant metastasis (P=0.000 07). However, there was no association between paxillin expression level with age, tumor histological type, tumor histological grade, clinical stage and menstruation status. Patients with high paxillin expression had significantly lower overall survival rate than patients with low paxillin expression(P=0.023). Conclusion: Paxillin was upregulated in cervical cancer tissues, which was associated with worse prognosis.
李娟1， 刘秋利2， 贾代林3， 李婧4， 邱海峰5， 王元3， 杨佩芳6. 桩蛋白在宫颈癌组织中的表达及其预后评估价值[J]. 江苏大学学报:医学版, 2017, 27(02): 168-171,175.
LI Juan1, LIU Qiu-li2, JIA Dai-lin3, LI Jing4, QIU Hai-feng5, WANG Yuan3, YANG Pei-fang6. Expression of paxillin in cervical cancer and its prognostic value. Journal of Jiangsu University(Medicine Edition), 2017, 27(02): 168-171,175.
[1\]Ferlay J, Soerjomataram I, Dikshit R, et al. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012\[J\]. Int J Cancer, 2015, 136(5): E359-E386.\[2\]Chen W, Zheng R, Baade PD, et al. Cancer statistics in China, 2015\[J\]. CA Cancer J Clin, 2016, 66 (2):115-132.\[3\]Rajkumar T, Sabitha K, Vijayalakshmi N, et al. Identification and validation of genes involved in cervical tumourigenesis\[J\]. BMC Cancer, 2011, 11: 80.\[4\]Glenney JR Jr, Zokas L. Novel tyrosine kinase substrates from Rous sarcoma virustransformed cells are present in the membrane skeleton\[J\]. J Cell Biol, 1989, 108(6): 2401-2408.\[5\]Hagel M, George EL, Kim A, et al. The adaptor protein paxillin is essential for normal development in the mouse and is a critical transducer of fibronectin signaling\[J\]. Mol Cell Biol, 2002, 22(3): 901-915.\[6\]Brown MC, Turner CE. Paxillin: adapting to change\[J\]. Physiol Rev, 2004, 84 (4):1315-1339.\[7\]Deakin NO,Turner CE. Paxillin comes of age\[J\]. J Cell Sci, 2008, 121(Pt 15):2435-2444.\[8\]Brown MC, Cary LA, Jamieson JS, et al. Src and FAK kinases cooperate to phosphorylate paxillin kinase linker, stimulate its focal adhesion localization, and regulate cell spreading and protrusiveness\[J\]. Mol Biol Cell, 2005, 16(9): 4316-4328.\[9\]Brimer N, Wade R, Vande Pol S. Interactions between E6, FAK, and GIT1 at paxillin LD4 are necessary for transformation by bovine papillomavirus 1 E6\[J\]. J Virol, 2014, 88(17): 9927-9933.\[10\]Cheng K, Kurzrock R, Qiu X, et al. Reduced focal adhesion kinase and paxillin phosphorylation in BCRABLtransfected cells\[J\]. Cancer, 2002,95(2): 440-450.\[11\]Deakin NO, Pignatelli J, Turner CE, et al. Diverse roles for the paxillin family of proteins in cancer\[J\].Genes Cancer, 2012, 3(5/6): 362-370.\[12\]Li L, Wang J, Gao L, et al. Expression of paxillin in laryngeal squamous cell carcinoma and its prognostic value\[J\]. Int J Clin Exp Pathol, 2015, 8(8): 9232-9239.\[13\]Chen DL, Wang ZQ, Ren C, et al. Abnormal expression of paxillin correlates with tumor progression and poor survival in patients with gastric cancer\[J\]. J Transl Med, 2013, 11: 277.\[14\]Zhao CJ, Du SK, Dang XB, et al. Expression of paxillin is correlated with clinical prognosis in colorectal cancer patients\[J\]. Med Sci Monit, 2015, 21: 1989-1995.\[15\]梅琳琳. paxillin蛋白在宫颈鳞癌组织中的表达及其与宫颈鳞癌浸润转移的关系\[J\].中国实用医药，2012，7(36):42-43.\[16\]Schaller MD. Paxillin: a focal adhesionassociated adaptor protein\[J\]. Oncogene, 2001, 20(44): 6459-6472.\[17\]Li D, Li Z, Xiong J, et al. MicroRNA212 functions as an epigeneticsilenced tumor suppressor involving in tumor metastasis and invasion of gastric cancer throughdownregulating PXN expression\[J\]. Am J Cancer Res, 2015, 5(10): 2980-2997.\[18\]Sen A, De Castro I, Defranco DB, et al. Paxillin mediates extranuclear and intranuclear signaling in prostate cancer proliferation\[J\]. J Clin Invest, 2012, 122(7): 2469-2481.\[19\]Chen B, Xia L, Xu CS, et al. Paxillin functions as an oncogene in human gliomas by promoting cell migration and invasion\[J\]. Onco Targets Ther, 2016, 9: 6935-6943.\[20\]Qin J, Wang F, Jiang H , et al. MicroRNA145 suppresses cell migration and invasion by targeting paxillin in human colorectal cancer cells\[J\]. Int J Clin Exp Pathol, 2015, 8(2):1328-1340.\[21\]Walboomers JM, Jacobs MV, Manos MM, et al. Human papillomavirus is a necessary cause of invasive cervical cancer woldwide\[J\]. J Pathol,1999, 189(1):12-19.\[22\]Vande Pol SB, Brown MC, Turner CE. Association of Bovine Papillomavirus Type 1 E6 ontoprotein with the focal adhesion protein paxillin through a conserved protein interaction motif\[J\]. Oncogene, 1998, 16(1):43-52.\[23\]Wu DW, Cheng YW, Wang J, et al. Paxillin predicts survival and relapse in nonsmall cell lung cancer by microRNA218 targeting\[J\]. Cancer Res, 2010, 70(24): 10392-10401.\[24\]Wu DW, Chuang CY, Lin WL, et al. Paxillin promotes tumor progression and predicts survival and relapse in oral cavity squamous cell carcinoma by microRNA218 targeting\[J\]. Carcinogenesis, 2014, 35(8):1823-1829.\[25\]Yu J,Wang Y, Dong R, et al. Circulating microRNA218 was reduced in cervical cancer and correlated with tumor invasion\[J\]. J Cancer Res Clin Oncol, 2012, 138(4): 671-674.\[26\]Martinez I, Gardiner AS, Board KF, et al. Human papillomavirus type 16 reduces the expression of microRNA218 in cervical carcinoma cells\[J\]. Oncogene, 2008, 27(18): 2575-2582.
2017, Vol. 27 
