Expression and clinical significance of IL-7 in peripheral blood and intestines tissue of the colorectal disorders
TU Qiang1,2, PENG Qi-song2, JIN Jie1
(1. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2. Department of Clinical Laboratory, Jiangning Hospital of Nanjing, Nanjing Jiangsu 211100, China)
Abstract:Objective: To investigate the expression of IL-7 in peripheral blood and intestinal tissues and its clinical significance. Methods:The expression level of IL-7 in peripheral blood of 20 patients with ulcerative colitis and 27 patients with colorectal cancer were detected by ELISA.Thirteen healthy volunteers were used as controls.The immunohistochemical method was also used to detect the intestinal tissue expression of IL-7 in 20 cases of ulcerative colitis and 27 cases of colorectal cancer. The corresponding adjacent normal tissues were collected as controls. Results:The level of IL-7 in peripheral blood of patients with colorectal cancer is (2.98±0.93) ng/L, which is significantly lower than that of patients with enteritis \[(4.72±0.69) ng/L,P<0.01\] and healthy controls[(5.64±0.24)ng/L,P<0.01]. The expression of IL-7 was strongly positive in the intestinal carcinoma tissue of 14 colorectal cancer patients(51.85%) and was weakly positive only in the carcinoma adjacent tissues of corresponding 5 colorectal cancer patients (18.52%) and in the colorectal mucosa of 5 patients with enteritis(25.00%). The expression of IL-7 in colorectal cancer tissues was significantly higher than that in its adjacent tissues(χ2=8.333,P<0.05), and also significantly higher than that in intestinal mucosa tissue of ulcerative colitis(χ2=4.454,P<0.05). Conclusion:The expression of IL-7 in peripheral blood and intestinal tissue can be used as a candidate diagnostic index for clinical intestinal diseases.
收稿日期: 2016-09-09
通讯作者:
金洁(通讯作者),教授,硕士生导师,E-mail: jinjie9@163.com
作者简介: 屠强(1979-),男,江苏南京人,副主任技师
引用本文:
屠强1,2, 彭启松2, 金洁1. 白介素7在结直肠癌患者外周血和癌组织中的表达及其临床意义[J]. 江苏大学学报:医学版, 2016, 26(05): 437-439.
TU Qiang1,2, PENG Qisong2, JIN Jie1. Expression and clinical significance of IL-7 in peripheral blood and intestines tissue of the colorectal disorders. Journal of Jiangsu University(Medicine Edition), 2016, 26(05): 437-439.
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