Expression of glycolysis related gene in peripheral blood mononuclear cells of patients with rheumatoid arthritis
XIONG Yu-yun1, WANG Bei1, TAO Zhen1, WU Ling2, YOU Hai-yan1, WANG Wen-hong3, JIAO Zhi-jun1
(1.Department of Clinical Laboratory, 2. Department of Rheumatism, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001； 3. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013, China)
Abstract：Objective: To explore the mRNA expression and potential significance of glycolysis related gene in peripheral blood mononuclear cells(PBMC) of patients with rheumatoid arthritis(RA). Methods: PBMC were purified by Ficoll and glycolysis related genes mRNA expression was analysed by real-time PCR. Results: Compared with healthy control, PBMC from active RA patients significantly expressed higher level of glycolysis related genes including TPI，ENO，PKM and MCT. However，there were no significant differences in these genes between inactive group and control group. Conclusion: In RA patients, glycolysis related genes mRNA expression level were changed. Glycolysis may play an important role in the mechanism of RA.
熊御云, 王蓓, 陶真, 吴玲, 尤海燕, 王文红, 焦志军. 类风湿关节炎患者外周血单个核细胞中糖酵解相关基因的表达[J]. 江苏大学学报:医学版, 2014, 24(04): 351-354.
XIONG Yu-Yun, WANG Bei, TAO Zhen, WU Ling, YOU Hai-Yan, WANG Wen-Hong, JIAO Zhi-Jun. Expression of glycolysis related gene in peripheral blood mononuclear cells of patients with rheumatoid arthritis. Journal of Jiangsu University(Medicine Edition), 2014, 24(04): 351-354.
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2014, Vol. 24 
