Abstract:Objective: To explore the effect of extracellular vesicles (EVs) derived from mouse adipose-derived mesenchymal stem cells(ADSCs) on the biological behaviors of ovarian cancer ID8 cell line and its possible mechanism. Methods: ADSCs were isolated by enzyme digestion method, and identified by morphological characteristics, osteogenic and adipogenic multidirectional differentiation ability and flow cytometry surface marker detection; EVs were isolated and purified from supernatant of ADSCs by ultracentrifugation, and ADSC-EVs were identified by transmission electron microscopy, nanoparticle size analysis and Western blotting; ID8 cells were treated with ADSC-EVs at different concentrations (20 μg/mL and 40 μg/mL) and PBS as the control group. The effect of ADSC-EVs on the proliferation of ID8 cells was detected by plate cloning assay. Wound healing and Transwell test were used to detect the effect of ADSC-EVs on the migration ability of ID8 cells. The expression of epithelial-mesenchymal transition(EMT) and protein kinase B(AKT) related proteins in ID8 cells were detected by Western blotting. Results: The isolated ADSCs had typical stem cell morphology, could differentiate into osteogenesis and adipogenesis, and consistent with the general characteristics of the stem cell surface. ADSC-EVs expressed CD9 and CD81 proteins. Compared with the PBS group, the proliferation and migration of ID8 cells in ADSC-EVs group were significantly enhanced (P<0.05), and the proliferation and migration of ID8 cells in 40 μg/mL ADSC-EVS group was stronger than that in 20 μg/mL ADSCEVS group (P<0.05). With the increase of ADSC-EVS concentration, the expression of E-cadherin in ID8 cells was significantly decreased, while the expression of N-cadherin, vimentin and pAkt was greatly increased (P<0.05). Conclusion: ADSC-EVs may promote the EMT of ID8 cells by activating the phosphorylation of AKT signal, thereby enhancing their proliferation and migration ability.
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