人脐带间充质干细胞源胞外囊泡促进施万细胞增殖和迁移

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目的: 探讨人脐带间充质干细胞（human umbilical cord mesenchymal stem cells， hUCMSCs）源胞外囊泡（extracellular vesicles，EVs）对施万细胞增殖和迁移的影响。方法: 应用高速离心法从hUCMSCs上清液中分离和纯化EVs（hUCMSC-EVs），透射电镜观察EVs形态特征；蛋白质印迹法检测EVs标志性蛋白CD63和CD9的表达；通过CCK-8和Transwell小室实验检测不同浓度的hUCMSC-EVs对施万细胞增殖和迁移能力的影响；共聚焦显微镜观察原代施万细胞对EVs的内吞。结果： hUCMSC-EVs呈圆形或椭圆形，且表达特异性分子标志物CD9和CD63；与对照组相比，hUCMSC-EVs显著促进施万细胞增殖和迁移（P均<0.05）；hUCMSCEVs与施万细胞孵育后细胞膜融合。结论： hUCMSC-EVs可显著促进施万细胞的增殖和迁移且呈浓度依赖。

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	张欢妍1
	2
	马永宾2
	董利阳1
	张文哲1
	薛菲1
	单文琪1
	汪雪峰1

关键词 ：人脐带间充质干细胞, 胞外囊泡, 施万细胞, 增殖, 迁移, 周围神经, 再生机制

Abstract：Objective: To investigate the effect of extracellular vesicles (EVs) derived from human umbilical cord mesenchymal stem cells (hUCMSCs) on proliferation and migration of schwann cells. Methods: EVs was isolated and purified from supernatant of hUCMSCs by ultrahigh speed centrifugation, morphological characteristics of EVs were observed by transmission electron microscope. Western blotting was used to detect the expression of EVs marker proteins CD63 and CD9. The effects of different concentrations of hUCMSCEVs on proliferation and migration of schwann cells were tested by CCK8 and Transwell chamber experiments. Immunofluorescence assay was used to observe the fusion of EVs and primary schwann cells cell membranes. Results: hUCMSCEVs was round or oval and expressed specific molecular markers CD63 and CD9; Compared with the control group, hUCMSCEVs significantly promoted schwann cells proliferation (P<0.05) and migration (P<0.05). hUCMSCEVs fuse with cell membrane after incubation with schwann cells. Conclusion: hUCMSCEVs can significantly promote proliferation and migration of schwann cells in a concentrationdependent manner.

收稿日期: 2020-01-15

基金资助:国家自然科学基金资助项目（81871243，81900562）;常州市科技项目（CJ20180001）

通讯作者: 汪雪峰（通讯作者），研究员，博士生导师，E-mail:xuefengwang@ujs.edu.cn

作者简介: 张欢妍（1984—），女，硕士研究生；

引用本文:

张欢妍1,2，马永宾2，董利阳1，张文哲1，薛菲1，单文琪1，汪雪峰1. 人脐带间充质干细胞源胞外囊泡促进施万细胞增殖和迁移[J]. 江苏大学学报:医学版, 2020, 30(04): 307-311. ZHANG Huan-yan1,2， MA Yong-bin2， DONG Li-yang1， ZHANG Wen-zhe1，XUE fei1， SHAN Wen-qi1， WANG Xue-feng1.

Human umbilical cord mesenchymal stem cells-derived extracellular vesicles promote proliferation and migration of schwann cells

. Journal of Jiangsu University(Medicine Edition), 2020, 30(04): 307-311.

链接本文:

http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2020/V30/I04/307

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