非编码 RNA GlmY 对伤寒沙门菌生物膜形成的影响

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摘要 目的: 探究非编码 RNA GlmY 对伤寒沙门菌生物膜形成的影响及其分子机制。方法: 用自杀质粒pGMB151介导的同源重组方法，制备 glmY 缺陷株；用96孔微量板结晶紫染色法检测各菌株生物膜形成情况；通过qRTPCR分析glmY 缺陷株及野生株中生物膜形成相关基因的mRNA表达水平。结果： 成功制备glmY缺陷株；细菌生物膜形成实验表明，与野生株相比，glmY缺陷株生物膜形成能力明显下降；qRTPCR分析结果显示，GlmY缺失后卷曲菌毛合成相关基因csgD和csgA的mRNA表达水平明显下调。结论： 伤寒沙门菌非编码 RNA GlmY可通过上调csgD和csgA的表达促进卷曲菌毛合成，进而促进生物膜的形成。

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	李雪1
	赵昕2
	靳梦彤1
	唐浩1
	张盈1
	黄新祥1

关键词 ：伤寒沙门菌, 非编码 RNA, GlmY, 生物膜

Abstract：Objective: To investigate the effect of non-coding RNA GlmY on the biofilm formation of Salmonella enterica serovar Typhi (S. Typhi) and its potential molecular mechanisms.Methods: The homologous recombination induced by the suicide plasmid pGMB151 was used to generate the glmY deleted mutant of S. Typhi. The bacterial biofilm formation in 96-microwell plate was detected by crystal violet staining. qRT-PCR was used to analyze the mRNA expression levels of biofilm formation-related genes in glmY deleted mutant and wild-type strain. Results: The glmY deletion mutant strain was successfully constructed. Compared with the wild-type strain, the glmY deletion mutant showed significantly weaker ability of biofilm formation. The qRT-PCR results showed that mRNA level of csgD and csgA was significantly decreased after deletion of GlmY. Conclusion: The non-coding RNA GlmY may enhance the biofilm formation of S. Typhi by up-regulating the expression of csgD, csgA and Curli synthesis.

收稿日期: 2020-02-23

基金资助:国家自然科学基金资助项目(31670131)

通讯作者: 黄新祥(通讯作者)，教授，博士生导师，E-mail: huxinx@ujs.edu.cn

作者简介: 李雪(1995—)，女，硕士研究生

引用本文:

李雪1，赵昕2，靳梦彤1，唐浩1，张盈1，黄新祥1. 非编码 RNA GlmY 对伤寒沙门菌生物膜形成的影响[J]. 江苏大学学报:医学版, 2020, 30(05): 435-438. LI Xue1, ZHAO Xin2, JIN Meng-tong1, TANG Hao1, ZHANG Ying1, HUANG Xin-xiang1.

Effect of non-coding RNA GlmY on biofilm formation of Salmonella enterica serovar Typhi

. Journal of Jiangsu University(Medicine Edition), 2020, 30(05): 435-438.

链接本文:

http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2020/V30/I05/435

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