Abstract:[Abstract]Objective: To investigate the expression characteristics of ArpH under different environmental stress conditions and the effects of two component regulatory system genes and oxygen stress regulator gene on the expression of ArpH in Salmonella enterica serovar Typhi (S. Typhi). Methods: The ArpH expression of S. Typhi in different growth stages were analyzed by Northern blotting and qRT-PCR. qRT-PCR was used to analyze ArpH expression in S. Typhi under different stress conditions, including acidic, oxidative, hyperosmotic and heat stress. Effects of two component regulation system genes (ompR, rcsB) and oxygen stress regulator gene (oxyR) on the expression of ArpH in S. Typhi were analyzed by qRT-PCR. Results: The expression of ArpH was the highest in S. Typhi at the late logarithmic phase. The expression of ArpH increased gradually from the early logarithmic phase to the stable phase, and decreased after the stable phase. The expression level of ArpH decreased significantly under acidic stress and increased under oxidative stress, while the expression of ArpH was not affected by hyperosmotic stress and heat stress. When ompR, rcsB and oxyR genes were deleted separately, the expression of ArpH decreased significantly. Conclusion: The ArpH was beneficial to bacterial growth in the acid and oxidative stress,the genes ompR, rcsB and oxyR of S. Typhi had a positive regulatory effect on ArpH.
[2]Nieto PA, PardoRoa C, SalazarEchegarai FJ, et al. New insights about excisable pathogenicity islands in Salmonella and their contribution to virulence\[J\]. Microbes Infect, 2016,18(5):302-309.
[3]Klein G, Raina S. Small regulatory bacterial RNAs regulating the envelope stress response\[J\]. Biochem Soc Trans, 2017, 45(2):417-425.
[4]Lebreton A, Cossart P. RNA and proteinmediated control of Listeria monocytogenes virulence gene expression\[J\]. RNA Biol, 2017, 14(5):460-470.
[5]Chew CL, Conos SA, Unal B, et al. Noncoding RNAs: master regulators of inflammatory signaling\[J\]. Trends Mol Med, 2018, 24(1):66-84.
[6]Dadzie I, Ni B, Gong M, et al. Identification and characterization of a cis antisense RNA of the parC gene encoding DNA topoisomerase IV of Salmonella enterica serovar Typhi\[J\]. Res Microbiol, 2014,165(6):439-446.
[7]Gong M, Xu S, Jin Y, et al. 5′UTR of malS increases the invasive capacity of Salmonella enterica serovar Typhi by influencing the expression of bax\[J\]. Future Microbiol, 2015, 10(6):941-954.
[8]Zhang Q, Zhang Y, Zhang X, et al. The novel cisencoded antisense RNA AsrC positively regulates the expression of rpoErseABC operon and thus enhances the motility of Salmonella enterica serovar Typhi\[J\]. Front Microbiol, 2015, 6:990.
[10]Livak KJ, Schmittgen TD. Analysis of relative gene expression data using realtime quantitative PCR and the 2-ΔΔCT method\[J\]. Methods, 2001, 25(4):402-408.
[11]Colgan AM, Quinn HJ, Kary SC. Negative supercoiling of DNA by gyrase is inhibited in Salmonella enterica serovar Typhimurium during adaptation to acid stress\[J\]. Mol Microbiol, 2018, 107(6):734-746.
[12]Goodale BC, Hampton TH, Ford EN,et al. Profiling microRNA expression in Atlantic killifish (Fundulus heteroclitus) gill and responses to arsenic and hyperosmotic stress\[J\]. Aquat Toxicol, 2019, 206:142-153.
[13]Aitken S, Magi S, Alhendi AM, et al. Transcriptional dynamics reveal critical roles for noncoding RNAs in the immediateearly response\[J\]. PLoS Comput Biol, 2015, 11(4):e1004217.
[14]Jena R, Garg DK, Choudhury L, et al. Heterologous expression of an engineered protein domain acts as chaperone and enhances thermotolerance of Escherichia coli\[J\]. Int J Biol Macromol, 2018, 107(Pt B):2086-2093.
[15]Schmidl SR, Ekness F, Sofjan K, et al. Rewiring bacterial twocomponent systems by modular DNAbinding domain swapping\[J\]. Nat Chem Biol, 2019, 15(7):690-698.
[16]Caby M, BontempsGallo S, Gruau P, et al. The EnvZOmpR twocomponent signaling system is inactivated in a mutant devoid of osmoregulated periplasmic glucans in Dickeya dadantii\[J\].Front Microbiol, 2018,9:2459.