Expression of FLCN in cervical squamous cancer tissue and its effect on proliferation and apoptosis in SiHa cells" />
FLCN在子宫颈鳞癌组织中的表达及其对子宫颈鳞癌SiHa细胞增殖及凋亡的影响
Abstract：[Abstract]Objective: To investigate the expression of folliculin(FLCN) in cervical squamous cancer tissue and its effect on the cell proliferation and apoptosis in cervical carcinoma. Methods: The mRNA and protein expressions of FLCN in cervical squamous cancer tissue were detected by qRTPCR and immunohistochemical technique. After overexpression or knocking down of FLCN in cervical squamous cancer SiHa cells, the FLCN mRNA expression was detected by qRT-PCR, the cell proliferation was evaluated by CCK8 -and phosphorylated histone H3 (PH3) staining, the cell apoptosis was measured by TUNEL. Results: Compared with the control group, FLCN mRNA and protein level expression in cervical squamous cancer groups were significantly reduced (P<0.05). After FLCN siRNA was transfected, cell proliferation increased and apoptosis decreased, PH3 protein positive ratio increased, TUNEL positive cell ratio decreased (P<0.05 or <0.01 ). Afer overexpression of FLCN, cell proliferation was inhibited and apoptosis was enhanced, the proportion of PH3 proteinpositive cells decreased, and the proportion of TUNELp-ositive cells increased(all P<0.01). Conclusion: FLCN gene was lowexpression in cervical squamous cancer tissue, which could inhibit proliferation and promote apoptosis of SiHa cells.
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